The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil
Background and Objective: Petroleum hydrocarbons are a bunch of pollutants hazardous to the environment. Nowadays, biodegradation of petroleum contaminants is considered as one of the most efficient and most cost effective methods of removing oil contamination from the environment. The main objectiv...
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| Format: | Article |
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Tehran University of Medical Sciences
2014-05-01
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| Series: | سلامت و محیط |
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| Online Access: | http://ijhe.tums.ac.ir/browse.php?a_code=A-10-25-5089&slc_lang=en&sid=1 |
| _version_ | 1818586117046272000 |
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| author | M Safari S Ahmady-Asbchin N Soltani |
| author_facet | M Safari S Ahmady-Asbchin N Soltani |
| author_sort | M Safari |
| collection | DOAJ |
| description | Background and Objective: Petroleum hydrocarbons are a bunch of pollutants hazardous to the environment. Nowadays, biodegradation of petroleum contaminants is considered as one of the most efficient and most cost effective methods of removing oil contamination from the environment. The main objective of this study is to investigate the potential of cyanobacterium Schizothrix vaginata ISC108 in the biodegradation of crude oil and to evaluate oil effects on growth rates, dry weight, and chlorophyll content.
Material and Methods: In this experimental study, the cyanobacterium Schizothrix vaginata ISC108 was obtained from the algal culture collection of Research Institute of Applied Science, ACECR, Tehran, Iran. First, purification of the cyanobacterium was performed using agar plate method on solid medium of BG11. Growth rate, chlorophyll content and dry weight of cyanobacteriaum was measured using spectrophotometry method. The biodegradation rate of crude oil was calculated using gas chromatography (GC) analysis.
Results: Measuring the growth rate of cyanobacterium Schizothrix vaginata at 1% treatment of crude oil and control samples showed that the growth of cyanobacterium in the presence of crude oil as the sole carbon source increases at the same rate as the control sample. Moreover, it was found that increasing the concentration of crude oil will result in increasing dry weight of Schizothrix vaginata; however, and the chlorophyll content reduced in various crude oil treatments. The average biodegradation of petroleum hydrocarbon after 14 and 28 days of treatment was 54.78 and 93.98% respectively.
Conclusions: It was found that cyanobacterium Schizothrix vaginata ISC108 has great potential in biodegradation of crude oil. Therefore, since oil is a product toxic to biological systems and is one of the main pollutants of bioecosystem, it has a great potential to be used as an indicator to eliminate pollution in contaminated areas. |
| first_indexed | 2024-12-16T08:47:51Z |
| format | Article |
| id | doaj.art-0a70fad51b224e9a97387debb7372b88 |
| institution | Directory Open Access Journal |
| issn | 2008-2029 2008-3718 |
| language | fas |
| last_indexed | 2024-12-16T08:47:51Z |
| publishDate | 2014-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | سلامت و محیط |
| spelling | doaj.art-0a70fad51b224e9a97387debb7372b882022-12-21T22:37:34ZfasTehran University of Medical Sciencesسلامت و محیط2008-20292008-37182014-05-0173363374The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oilM Safari0S Ahmady-Asbchin1N Soltani2 Department of biology, Faculty of Basic Science, Ilam University, Ilam, Iran Department of Cell and Molecular Biology, Faculty of Basic Science, Mazandaran University, Babolsar, Iran Department of petroleum microbiology, Research Institute of applied science, ACECR, Tehran, Iran Background and Objective: Petroleum hydrocarbons are a bunch of pollutants hazardous to the environment. Nowadays, biodegradation of petroleum contaminants is considered as one of the most efficient and most cost effective methods of removing oil contamination from the environment. The main objective of this study is to investigate the potential of cyanobacterium Schizothrix vaginata ISC108 in the biodegradation of crude oil and to evaluate oil effects on growth rates, dry weight, and chlorophyll content. Material and Methods: In this experimental study, the cyanobacterium Schizothrix vaginata ISC108 was obtained from the algal culture collection of Research Institute of Applied Science, ACECR, Tehran, Iran. First, purification of the cyanobacterium was performed using agar plate method on solid medium of BG11. Growth rate, chlorophyll content and dry weight of cyanobacteriaum was measured using spectrophotometry method. The biodegradation rate of crude oil was calculated using gas chromatography (GC) analysis. Results: Measuring the growth rate of cyanobacterium Schizothrix vaginata at 1% treatment of crude oil and control samples showed that the growth of cyanobacterium in the presence of crude oil as the sole carbon source increases at the same rate as the control sample. Moreover, it was found that increasing the concentration of crude oil will result in increasing dry weight of Schizothrix vaginata; however, and the chlorophyll content reduced in various crude oil treatments. The average biodegradation of petroleum hydrocarbon after 14 and 28 days of treatment was 54.78 and 93.98% respectively. Conclusions: It was found that cyanobacterium Schizothrix vaginata ISC108 has great potential in biodegradation of crude oil. Therefore, since oil is a product toxic to biological systems and is one of the main pollutants of bioecosystem, it has a great potential to be used as an indicator to eliminate pollution in contaminated areas.http://ijhe.tums.ac.ir/browse.php?a_code=A-10-25-5089&slc_lang=en&sid=1Cyanobacteria Biodegradable Crude oil Carcinogenicity Gas chromatography |
| spellingShingle | M Safari S Ahmady-Asbchin N Soltani The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil سلامت و محیط Cyanobacteria Biodegradable Crude oil Carcinogenicity Gas chromatography |
| title | The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil |
| title_full | The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil |
| title_fullStr | The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil |
| title_full_unstemmed | The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil |
| title_short | The potential of cyanobacterium Schizothrix vaginata ISC108 in biodegradation of crude oil |
| title_sort | potential of cyanobacterium schizothrix vaginata isc108 in biodegradation of crude oil |
| topic | Cyanobacteria Biodegradable Crude oil Carcinogenicity Gas chromatography |
| url | http://ijhe.tums.ac.ir/browse.php?a_code=A-10-25-5089&slc_lang=en&sid=1 |
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