Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models
Abstract Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02293-x |
| _version_ | 1797801550421164032 |
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| author | Jiehan Li Christopher Jin Stefan Gustafsson Abhiram Rao Martin Wabitsch Chong Y. Park Thomas Quertermous Joshua W. Knowles Ewa Bielczyk-Maczynska |
| author_facet | Jiehan Li Christopher Jin Stefan Gustafsson Abhiram Rao Martin Wabitsch Chong Y. Park Thomas Quertermous Joshua W. Knowles Ewa Bielczyk-Maczynska |
| author_sort | Jiehan Li |
| collection | DOAJ |
| description | Abstract Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study the molecular regulation of adipogenesis, in particular the immortalized mouse 3T3-L1 line and the primary human Simpson-Golabi-Behmel syndrome (SGBS) line. However, the cell-to-cell variability of transcriptional changes prior to and during adipogenesis in these models is not well understood. Here, we present a single-cell RNA-Sequencing (scRNA-Seq) dataset collected before and during adipogenic differentiation of 3T3-L1 and SGBS cells. To minimize the effects of experimental variation, we mixed 3T3-L1 and SGBS cells and used computational analysis to demultiplex transcriptomes of mouse and human cells. In both models, adipogenesis results in the appearance of three cell clusters, corresponding to preadipocytes, early and mature adipocytes. These data provide a groundwork for comparative studies on these widely used in vitro models of human and mouse adipogenesis, and on cell-to-cell variability during this process. |
| first_indexed | 2024-03-13T04:52:39Z |
| format | Article |
| id | doaj.art-0a722d0cb0694bfa8551d9ed0044cc3d |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-03-13T04:52:39Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-0a722d0cb0694bfa8551d9ed0044cc3d2023-06-18T11:06:10ZengNature PortfolioScientific Data2052-44632023-06-0110111010.1038/s41597-023-02293-xSingle-cell transcriptome dataset of human and mouse in vitro adipogenesis modelsJiehan Li0Christopher Jin1Stefan Gustafsson2Abhiram Rao3Martin Wabitsch4Chong Y. Park5Thomas Quertermous6Joshua W. Knowles7Ewa Bielczyk-Maczynska8Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineDivision of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineClinical Epidemiology Unit, Department of Medical Sciences, Uppsala UniversityDepartment of Bioengineering, Stanford UniversityDepartment of Pediatrics and Adolescent Medicine, Center for Rare Endocrine Diseases, Division of Pediatric Endocrinology and Diabetes, Ulm University Medical CentreDivision of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineDivision of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineDivision of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineDivision of Cardiovascular Medicine, Department of Medicine, Stanford University School of MedicineAbstract Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study the molecular regulation of adipogenesis, in particular the immortalized mouse 3T3-L1 line and the primary human Simpson-Golabi-Behmel syndrome (SGBS) line. However, the cell-to-cell variability of transcriptional changes prior to and during adipogenesis in these models is not well understood. Here, we present a single-cell RNA-Sequencing (scRNA-Seq) dataset collected before and during adipogenic differentiation of 3T3-L1 and SGBS cells. To minimize the effects of experimental variation, we mixed 3T3-L1 and SGBS cells and used computational analysis to demultiplex transcriptomes of mouse and human cells. In both models, adipogenesis results in the appearance of three cell clusters, corresponding to preadipocytes, early and mature adipocytes. These data provide a groundwork for comparative studies on these widely used in vitro models of human and mouse adipogenesis, and on cell-to-cell variability during this process.https://doi.org/10.1038/s41597-023-02293-x |
| spellingShingle | Jiehan Li Christopher Jin Stefan Gustafsson Abhiram Rao Martin Wabitsch Chong Y. Park Thomas Quertermous Joshua W. Knowles Ewa Bielczyk-Maczynska Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models Scientific Data |
| title | Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| title_full | Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| title_fullStr | Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| title_full_unstemmed | Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| title_short | Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| title_sort | single cell transcriptome dataset of human and mouse in vitro adipogenesis models |
| url | https://doi.org/10.1038/s41597-023-02293-x |
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