Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure
Several anthropogenic products in wastewater are considered a threat to the aquatic environment. In addition to common industrial pollutants, levels of pharmaceuticals have been increasingly found in the environment in recent years, which may present a strong risk to the aquatic species that live th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/7/2/67 |
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| author | Davide Di Paola Carmelo Iaria Giovanni Lanteri Marika Cordaro Rosalia Crupi Rosalba Siracusa Ramona D’Amico Roberta Fusco Daniela Impellizzeri Salvatore Cuzzocrea Nunziacarla Spanò Enrico Gugliandolo Alessio Filippo Peritore |
| author_facet | Davide Di Paola Carmelo Iaria Giovanni Lanteri Marika Cordaro Rosalia Crupi Rosalba Siracusa Ramona D’Amico Roberta Fusco Daniela Impellizzeri Salvatore Cuzzocrea Nunziacarla Spanò Enrico Gugliandolo Alessio Filippo Peritore |
| author_sort | Davide Di Paola |
| collection | DOAJ |
| description | Several anthropogenic products in wastewater are considered a threat to the aquatic environment. In addition to common industrial pollutants, levels of pharmaceuticals have been increasingly found in the environment in recent years, which may present a strong risk to the aquatic species that live there. The constant consumption of biologically active chemicals for human health has been matched by an increase in the leaking of these compounds in natural habitats over the last two decades. This study is aimed at evaluating the developmental toxicity of fotemustine in the ecological environment. Zebrafish embryos were exposed to doses of 25, 50 and 100 µg/mL from 4 h post-fertilization to 120 h. This study confirms that fotemustine exposure at 50 and 100 µg/mL affects the survival and hatching rate, morphology score and body length. Additionally, it significantly disturbs the antioxidant defense system and increases ROS in zebrafish larvae. From the molecular point of view, fotemustine exposure strongly induces apoptosis, endoplasmic reticulum stress (ERS) and the Wnt signaling pathway. |
| first_indexed | 2024-03-09T10:36:53Z |
| format | Article |
| id | doaj.art-0a7456796c3b4bdfb6351891dead5550 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T10:36:53Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-0a7456796c3b4bdfb6351891dead55502023-12-01T20:52:47ZengMDPI AGFishes2410-38882022-03-01726710.3390/fishes7020067Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine ExposureDavide Di Paola0Carmelo Iaria1Giovanni Lanteri2Marika Cordaro3Rosalia Crupi4Rosalba Siracusa5Ramona D’Amico6Roberta Fusco7Daniela Impellizzeri8Salvatore Cuzzocrea9Nunziacarla Spanò10Enrico Gugliandolo11Alessio Filippo Peritore12Department of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98166 Messina, ItalyDepartment of Veterinary Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98166 Messina, ItalyDepartment of Veterinary Science, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, ItalySeveral anthropogenic products in wastewater are considered a threat to the aquatic environment. In addition to common industrial pollutants, levels of pharmaceuticals have been increasingly found in the environment in recent years, which may present a strong risk to the aquatic species that live there. The constant consumption of biologically active chemicals for human health has been matched by an increase in the leaking of these compounds in natural habitats over the last two decades. This study is aimed at evaluating the developmental toxicity of fotemustine in the ecological environment. Zebrafish embryos were exposed to doses of 25, 50 and 100 µg/mL from 4 h post-fertilization to 120 h. This study confirms that fotemustine exposure at 50 and 100 µg/mL affects the survival and hatching rate, morphology score and body length. Additionally, it significantly disturbs the antioxidant defense system and increases ROS in zebrafish larvae. From the molecular point of view, fotemustine exposure strongly induces apoptosis, endoplasmic reticulum stress (ERS) and the Wnt signaling pathway.https://www.mdpi.com/2410-3888/7/2/67fotemustinezebrafishROSdevelopmental toxicity |
| spellingShingle | Davide Di Paola Carmelo Iaria Giovanni Lanteri Marika Cordaro Rosalia Crupi Rosalba Siracusa Ramona D’Amico Roberta Fusco Daniela Impellizzeri Salvatore Cuzzocrea Nunziacarla Spanò Enrico Gugliandolo Alessio Filippo Peritore Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure Fishes fotemustine zebrafish ROS developmental toxicity |
| title | Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure |
| title_full | Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure |
| title_fullStr | Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure |
| title_full_unstemmed | Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure |
| title_short | Sensitivity of Zebrafish Embryogenesis to Risk of Fotemustine Exposure |
| title_sort | sensitivity of zebrafish embryogenesis to risk of fotemustine exposure |
| topic | fotemustine zebrafish ROS developmental toxicity |
| url | https://www.mdpi.com/2410-3888/7/2/67 |
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