Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers
Lactobacillus plantarum has recently been found to be a natural source feed additive bacteria with great advantages in food safety and animal welfare. Discovering novel strains with commercial application potentiation could benefit the local poultry industry, and in particular support Chinese farmer...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1195382/full |
| _version_ | 1827910017717633024 |
|---|---|
| author | Yangyan Yin Yangyan Yin Yangyan Yin Yuying Liao Yuying Liao Jun Li Jun Li Zhe Pei Leping Wang Leping Wang Leping Wang Yan Shi Yan Shi Yan Shi Hongyan Peng Hongyan Peng Hongyan Peng Yizhou Tan Yizhou Tan Yizhou Tan Changting Li Changting Li Huili Bai Huili Bai Chunxia Ma Chunxia Ma Yu Gong Tianchao Wei Hao Peng Hao Peng |
| author_facet | Yangyan Yin Yangyan Yin Yangyan Yin Yuying Liao Yuying Liao Jun Li Jun Li Zhe Pei Leping Wang Leping Wang Leping Wang Yan Shi Yan Shi Yan Shi Hongyan Peng Hongyan Peng Hongyan Peng Yizhou Tan Yizhou Tan Yizhou Tan Changting Li Changting Li Huili Bai Huili Bai Chunxia Ma Chunxia Ma Yu Gong Tianchao Wei Hao Peng Hao Peng |
| author_sort | Yangyan Yin |
| collection | DOAJ |
| description | Lactobacillus plantarum has recently been found to be a natural source feed additive bacteria with great advantages in food safety and animal welfare. Discovering novel strains with commercial application potentiation could benefit the local poultry industry, and in particular support Chinese farmers. In this study, we tested a recently isolated novel strain of Lactobacillus plantarum GX17 as a feed additive on the growth performance and intestinal barrier functions of 1-day-old Chinese yellow-feather chicks. As good as other commercial probiotics, feeding with Lactobacillus plantarum GX17 showed significant improvements in humoral immune responses and enhanced the immune effect after vaccination for either the Newcastle disease vaccine or the avian influenza vaccine. This study also found that feeding with Lactobacillus plantarum GX17 improved the feed-to-weight ratio and caused a significant increase of the villus length to crypt depth ratio. Furthermore, Lactobacillus plantarum GX17 significantly up-regulated the mRNA expression of CLDN, MUC2, and TLR2, all of which are jejunum-associated barrier genes, indicating an improvement of the intestinal barrier functions by enhancing the tight junction between epithelia cells. These results are comparable to the effects of feeding the commercial complex probiotics that improve the expression levels of CLDN, ocludin, MUC2, TLR2, and TLR4. In terms of maintaining intestinal health, commercial complex probiotics increased the relative abundance of Parabacteroides and Romboutsia, while Lactobacillus plantarum GX17 increased the relative abundance of Pseudoflavonifractor. Our data suggest that Lactobacillus plantarum GX17 could enhance the intestinal absorption of nutrients and therefore improve the growth performance of Chinese yellow-feather chicks. In conclusion, compared with the commercial complex probiotics, Lactobacillus plantarum GX17 has more positive effects on the growth performance and intestinal barrier function of yellow-feather chickens, and can be used as a feed additive. |
| first_indexed | 2024-03-13T01:46:14Z |
| format | Article |
| id | doaj.art-0a75b192e11d4d2cb7da9660bae9790f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T01:46:14Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0a75b192e11d4d2cb7da9660bae9790f2023-07-03T07:41:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-07-011410.3389/fimmu.2023.11953821195382Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilersYangyan Yin0Yangyan Yin1Yangyan Yin2Yuying Liao3Yuying Liao4Jun Li5Jun Li6Zhe Pei7Leping Wang8Leping Wang9Leping Wang10Yan Shi11Yan Shi12Yan Shi13Hongyan Peng14Hongyan Peng15Hongyan Peng16Yizhou Tan17Yizhou Tan18Yizhou Tan19Changting Li20Changting Li21Huili Bai22Huili Bai23Chunxia Ma24Chunxia Ma25Yu Gong26Tianchao Wei27Hao Peng28Hao Peng29Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaVirginia Tech, Department of Engineering, Blacksburg, New York, NY, United StatesGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaGuizhou Provincial Livestock and Poultry Genetic Resources Management Station, Guiyang, ChinaInstitute of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of China(Guangxi)-Association of Southeast Asian Nations (ASEAN) Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, ChinaLactobacillus plantarum has recently been found to be a natural source feed additive bacteria with great advantages in food safety and animal welfare. Discovering novel strains with commercial application potentiation could benefit the local poultry industry, and in particular support Chinese farmers. In this study, we tested a recently isolated novel strain of Lactobacillus plantarum GX17 as a feed additive on the growth performance and intestinal barrier functions of 1-day-old Chinese yellow-feather chicks. As good as other commercial probiotics, feeding with Lactobacillus plantarum GX17 showed significant improvements in humoral immune responses and enhanced the immune effect after vaccination for either the Newcastle disease vaccine or the avian influenza vaccine. This study also found that feeding with Lactobacillus plantarum GX17 improved the feed-to-weight ratio and caused a significant increase of the villus length to crypt depth ratio. Furthermore, Lactobacillus plantarum GX17 significantly up-regulated the mRNA expression of CLDN, MUC2, and TLR2, all of which are jejunum-associated barrier genes, indicating an improvement of the intestinal barrier functions by enhancing the tight junction between epithelia cells. These results are comparable to the effects of feeding the commercial complex probiotics that improve the expression levels of CLDN, ocludin, MUC2, TLR2, and TLR4. In terms of maintaining intestinal health, commercial complex probiotics increased the relative abundance of Parabacteroides and Romboutsia, while Lactobacillus plantarum GX17 increased the relative abundance of Pseudoflavonifractor. Our data suggest that Lactobacillus plantarum GX17 could enhance the intestinal absorption of nutrients and therefore improve the growth performance of Chinese yellow-feather chicks. In conclusion, compared with the commercial complex probiotics, Lactobacillus plantarum GX17 has more positive effects on the growth performance and intestinal barrier function of yellow-feather chickens, and can be used as a feed additive.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1195382/fullLactobacillus plantarumyellow-feather broilergrowth performanceimmunityintestinal flora population |
| spellingShingle | Yangyan Yin Yangyan Yin Yangyan Yin Yuying Liao Yuying Liao Jun Li Jun Li Zhe Pei Leping Wang Leping Wang Leping Wang Yan Shi Yan Shi Yan Shi Hongyan Peng Hongyan Peng Hongyan Peng Yizhou Tan Yizhou Tan Yizhou Tan Changting Li Changting Li Huili Bai Huili Bai Chunxia Ma Chunxia Ma Yu Gong Tianchao Wei Hao Peng Hao Peng Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers Frontiers in Immunology Lactobacillus plantarum yellow-feather broiler growth performance immunity intestinal flora population |
| title | Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers |
| title_full | Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers |
| title_fullStr | Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers |
| title_full_unstemmed | Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers |
| title_short | Lactobacillus plantarum GX17 benefits growth performance and improves functions of intestinal barrier/intestinal flora among yellow-feathered broilers |
| title_sort | lactobacillus plantarum gx17 benefits growth performance and improves functions of intestinal barrier intestinal flora among yellow feathered broilers |
| topic | Lactobacillus plantarum yellow-feather broiler growth performance immunity intestinal flora population |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1195382/full |
| work_keys_str_mv | AT yangyanyin lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yangyanyin lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yangyanyin lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yuyingliao lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yuyingliao lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT junli lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT junli lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT zhepei lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT lepingwang lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT lepingwang lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT lepingwang lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yanshi lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yanshi lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yanshi lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT hongyanpeng lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT hongyanpeng lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT hongyanpeng lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yizhoutan lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yizhoutan lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yizhoutan lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT changtingli lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT changtingli lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT huilibai lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT huilibai lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT chunxiama lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT chunxiama lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT yugong lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT tianchaowei lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT haopeng lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers AT haopeng lactobacillusplantarumgx17benefitsgrowthperformanceandimprovesfunctionsofintestinalbarrierintestinalfloraamongyellowfeatheredbroilers |