Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis
The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups: Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis. Group 3 - 50 rats with simulated fecal peritonitis with subsequent anti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Kazimierz Wielki University
2019-06-01
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| Series: | Journal of Education, Health and Sport |
| Subjects: | |
| Online Access: | https://apcz.umk.pl/JEHS/article/view/33045 |
| _version_ | 1818047771737849856 |
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| author | I. V. Savytskyi S. V. Tsypoviaz R. G. Lenik O. V. Bilash R. H. Zashchuk I. V. Miastkivska L. M. Zaiats |
| author_facet | I. V. Savytskyi S. V. Tsypoviaz R. G. Lenik O. V. Bilash R. H. Zashchuk I. V. Miastkivska L. M. Zaiats |
| author_sort | I. V. Savytskyi |
| collection | DOAJ |
| description |
The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups:
Group 1 - 20 intact animals.
Group 2 - 50 rats with simulated fecal peritonitis.
Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution.
Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor.
Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826).
The following results were obtained.
Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis.
Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001).
It was revealed significant increase in erythrocyte intoxication index (EII).
It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis.
It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed.
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| first_indexed | 2024-12-10T10:11:05Z |
| format | Article |
| id | doaj.art-0a7a4d3630fe4862924c16df552364e8 |
| institution | Directory Open Access Journal |
| issn | 2391-8306 |
| language | English |
| last_indexed | 2024-12-10T10:11:05Z |
| publishDate | 2019-06-01 |
| publisher | Kazimierz Wielki University |
| record_format | Article |
| series | Journal of Education, Health and Sport |
| spelling | doaj.art-0a7a4d3630fe4862924c16df552364e82022-12-22T01:53:08ZengKazimierz Wielki UniversityJournal of Education, Health and Sport2391-83062019-06-0196Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitisI. V. Savytskyi0S. V. Tsypoviaz1R. G. Lenik2O. V. Bilash3R. H. Zashchuk4I. V. Miastkivska5L. M. Zaiats6Odessa National Medical UniversityOdessa National Medical UniversityState Enterprise Ukrainian Institute for Medicine of Transport of the Ministry of Health of UkraineOdessa National Medical UniversityOdessa National Medical UniversityOdessa National Medical UniversityIvano-Frankivsk National Medical University The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups: Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis. Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor. Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826). The following results were obtained. Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis. Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001). It was revealed significant increase in erythrocyte intoxication index (EII). It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis. It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed. https://apcz.umk.pl/JEHS/article/view/33045peritonitisexperimental researchvon Willebrand factorerythrocyte intoxication index (EII)leukocyte intoxication index (LII)L-arginin |
| spellingShingle | I. V. Savytskyi S. V. Tsypoviaz R. G. Lenik O. V. Bilash R. H. Zashchuk I. V. Miastkivska L. M. Zaiats Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis Journal of Education, Health and Sport peritonitis experimental research von Willebrand factor erythrocyte intoxication index (EII) leukocyte intoxication index (LII) L-arginin |
| title | Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| title_full | Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| title_fullStr | Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| title_full_unstemmed | Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| title_short | Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| title_sort | interdependence investigation of dynamic between the von willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis |
| topic | peritonitis experimental research von Willebrand factor erythrocyte intoxication index (EII) leukocyte intoxication index (LII) L-arginin |
| url | https://apcz.umk.pl/JEHS/article/view/33045 |
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