Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine
A probiotic strain Lactobacillus paracasei L9,which was isolated from human intestine, was investigated for its immunomodulatory activity in vivo. Results showed that L9 improved systemic immunity by enhancing the phagocytic activity of peritoneal macrophages, the proliferation ratio of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600021Z.pdf |
| Summary: | A probiotic strain Lactobacillus paracasei L9,which was isolated from human
intestine, was investigated for its immunomodulatory activity in
vivo. Results showed that L9 improved systemic immunity by enhancing the
phagocytic activity of peritoneal macrophages, the proliferation ratio of
splenocytes, the IgG level in the serum and the level of IgA in the mucosa.
Further, L9induced theTh1-polarized immune response by elevating the
IFN-γ/IL-4 ratio in the mucosa. This effect was confirmed by the enhanced
IL-12-inducing activity of macrophages after in vitro stimulation of L9. Also
detected was increased expression of TLR-2mRNA in the mucosa. We predict
that L9 could enhance innate immunity by activating TLR-2 in the mucosa, and
enhance acquired immunity by promoting Th1 polarization through
induced production of IL-12 by macrophages. |
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| ISSN: | 0354-4664 1821-4339 |