Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine
A probiotic strain Lactobacillus paracasei L9,which was isolated from human intestine, was investigated for its immunomodulatory activity in vivo. Results showed that L9 improved systemic immunity by enhancing the phagocytic activity of peritoneal macrophages, the proliferation ratio of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600021Z.pdf |
| _version_ | 1811200411509456896 |
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| author | Zhu Yuanbo Zhu Jun Zhao Liang Zhang Ming Guo Huiyuan Ren Fazheng |
| author_facet | Zhu Yuanbo Zhu Jun Zhao Liang Zhang Ming Guo Huiyuan Ren Fazheng |
| author_sort | Zhu Yuanbo |
| collection | DOAJ |
| description | A probiotic strain Lactobacillus paracasei L9,which was isolated from human
intestine, was investigated for its immunomodulatory activity in
vivo. Results showed that L9 improved systemic immunity by enhancing the
phagocytic activity of peritoneal macrophages, the proliferation ratio of
splenocytes, the IgG level in the serum and the level of IgA in the mucosa.
Further, L9induced theTh1-polarized immune response by elevating the
IFN-γ/IL-4 ratio in the mucosa. This effect was confirmed by the enhanced
IL-12-inducing activity of macrophages after in vitro stimulation of L9. Also
detected was increased expression of TLR-2mRNA in the mucosa. We predict
that L9 could enhance innate immunity by activating TLR-2 in the mucosa, and
enhance acquired immunity by promoting Th1 polarization through
induced production of IL-12 by macrophages. |
| first_indexed | 2024-04-12T02:04:12Z |
| format | Article |
| id | doaj.art-0a7c437c6cf745ce96101fcc17608f1c |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-12T02:04:12Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-0a7c437c6cf745ce96101fcc17608f1c2022-12-22T03:52:34ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168231131810.2298/ABS150216021Z0354-46641600021ZEffect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestineZhu Yuanbo0Zhu Jun1Zhao Liang2Zhang Ming3Guo Huiyuan4Ren Fazheng5China Agricultural University, College of Food Science and Nutritional Engineering, Beijing, ChinaKey Laboratory of Functional Dairy, Beijing, ChinaKey Laboratory of Functional Dairy, Beijing, ChinaBeijing Technology and Business University, School of Food and Chemical Engineering, Beijing, ChinaKey Laboratory of Functional Dairy, Beijing, ChinaChina Agricultural University, College of Food Science and Nutritional Engineering, Beijing, ChinaA probiotic strain Lactobacillus paracasei L9,which was isolated from human intestine, was investigated for its immunomodulatory activity in vivo. Results showed that L9 improved systemic immunity by enhancing the phagocytic activity of peritoneal macrophages, the proliferation ratio of splenocytes, the IgG level in the serum and the level of IgA in the mucosa. Further, L9induced theTh1-polarized immune response by elevating the IFN-γ/IL-4 ratio in the mucosa. This effect was confirmed by the enhanced IL-12-inducing activity of macrophages after in vitro stimulation of L9. Also detected was increased expression of TLR-2mRNA in the mucosa. We predict that L9 could enhance innate immunity by activating TLR-2 in the mucosa, and enhance acquired immunity by promoting Th1 polarization through induced production of IL-12 by macrophages.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600021Z.pdfLactobacillus paracasei L9immunomodulatory activitysystemic immunityTh1 immunityTLR-2 |
| spellingShingle | Zhu Yuanbo Zhu Jun Zhao Liang Zhang Ming Guo Huiyuan Ren Fazheng Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine Archives of Biological Sciences Lactobacillus paracasei L9 immunomodulatory activity systemic immunity Th1 immunity TLR-2 |
| title | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine |
| title_full | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine |
| title_fullStr | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine |
| title_full_unstemmed | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine |
| title_short | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine |
| title_sort | effect of oral administration of lactobacillus paracasei l9 on mouse systemic immunity and the immune response in the intestine |
| topic | Lactobacillus paracasei L9 immunomodulatory activity systemic immunity Th1 immunity TLR-2 |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600021Z.pdf |
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