RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO
Phosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most common media used in embryo recovery. The aim of our paper was to investigate if the recovery media used for the recovery of the mouse embryo is influencing in vitro developmental capacity. As biological material we used 10 use...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1141 |
| Summary: | Phosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most
common media used in embryo recovery. The aim of our paper was to investigate if
the recovery media used for the recovery of the mouse embryo is influencing in vitro
developmental capacity. As biological material we used 10 used were mouse
females, age 2 months superovulated with 5UI PMSG (Pregnant Mare Serum
Gonadotropine) and 5 UI hCG (human Corionic Gonadotropine). The embryos used
were recovered, by oviduct flushing, at 24 hours from the identification of the
vaginal plug. The majority of the embryos (78.3%) were in two cells stage. A total of
123, 2 cells embryos were cultivated in M16 medium. The evolution of the embryos
was examined at 24, 48 and 72 hours interval. The proportion of hatched blastocyst
was higher at the embryos recovered with M2 (53.7%) compared with the embryos
recovered with PBS 0.4% BSA. The difference is statistically very significant
(p<0.001). Embryos recovered in M2 media have a higher in vitro developmental
capacity compared with the embryos recovered in PBS media supplemented with
0,4% BSA, possibly because of the sodium bicarbonate and lactate used in M2 media
for pH regulation. |
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| ISSN: | 1841-9364 2344-4576 |