RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO
Phosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most common media used in embryo recovery. The aim of our paper was to investigate if the recovery media used for the recovery of the mouse embryo is influencing in vitro developmental capacity. As biological material we used 10 use...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1141 |
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| author | ADA CEAN N. PĂCALĂ ALEXANDRA BOLEMAN |
| author_facet | ADA CEAN N. PĂCALĂ ALEXANDRA BOLEMAN |
| author_sort | ADA CEAN |
| collection | DOAJ |
| description | Phosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most
common media used in embryo recovery. The aim of our paper was to investigate if
the recovery media used for the recovery of the mouse embryo is influencing in vitro
developmental capacity. As biological material we used 10 used were mouse
females, age 2 months superovulated with 5UI PMSG (Pregnant Mare Serum
Gonadotropine) and 5 UI hCG (human Corionic Gonadotropine). The embryos used
were recovered, by oviduct flushing, at 24 hours from the identification of the
vaginal plug. The majority of the embryos (78.3%) were in two cells stage. A total of
123, 2 cells embryos were cultivated in M16 medium. The evolution of the embryos
was examined at 24, 48 and 72 hours interval. The proportion of hatched blastocyst
was higher at the embryos recovered with M2 (53.7%) compared with the embryos
recovered with PBS 0.4% BSA. The difference is statistically very significant
(p<0.001). Embryos recovered in M2 media have a higher in vitro developmental
capacity compared with the embryos recovered in PBS media supplemented with
0,4% BSA, possibly because of the sodium bicarbonate and lactate used in M2 media
for pH regulation. |
| first_indexed | 2024-04-24T11:31:38Z |
| format | Article |
| id | doaj.art-0a7ce4e615bb4b6ab9f4a19b69b8e870 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:31:38Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-0a7ce4e615bb4b6ab9f4a19b69b8e8702024-04-10T08:53:46ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-01421881141RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYOADA CEAN0N. PĂCALĂ1ALEXANDRA BOLEMAN2Faculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, TimisoaraPhosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most common media used in embryo recovery. The aim of our paper was to investigate if the recovery media used for the recovery of the mouse embryo is influencing in vitro developmental capacity. As biological material we used 10 used were mouse females, age 2 months superovulated with 5UI PMSG (Pregnant Mare Serum Gonadotropine) and 5 UI hCG (human Corionic Gonadotropine). The embryos used were recovered, by oviduct flushing, at 24 hours from the identification of the vaginal plug. The majority of the embryos (78.3%) were in two cells stage. A total of 123, 2 cells embryos were cultivated in M16 medium. The evolution of the embryos was examined at 24, 48 and 72 hours interval. The proportion of hatched blastocyst was higher at the embryos recovered with M2 (53.7%) compared with the embryos recovered with PBS 0.4% BSA. The difference is statistically very significant (p<0.001). Embryos recovered in M2 media have a higher in vitro developmental capacity compared with the embryos recovered in PBS media supplemented with 0,4% BSA, possibly because of the sodium bicarbonate and lactate used in M2 media for pH regulation.https://spasb.ro/index.php/public_html/article/view/1141embryosrecovery mediam2pbs-0,4%bsahatching rate |
| spellingShingle | ADA CEAN N. PĂCALĂ ALEXANDRA BOLEMAN RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO Scientific Papers Animal Science and Biotechnologies embryos recovery media m2 pbs-0,4%bsa hatching rate |
| title | RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO |
| title_full | RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO |
| title_fullStr | RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO |
| title_full_unstemmed | RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO |
| title_short | RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO |
| title_sort | researches regarding the influence of recovery media on the in vitro development capacity of the preimplantational mouse embryo |
| topic | embryos recovery media m2 pbs-0,4%bsa hatching rate |
| url | https://spasb.ro/index.php/public_html/article/view/1141 |
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