The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom
The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VL...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SciELO
2008-01-01
|
| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300004 |
| _version_ | 1818033862636208128 |
|---|---|
| author | DM Dourado R Matias MF Almeida KR De Paula RP Vieira LVF Oliveira PTC Carvalho |
| author_facet | DM Dourado R Matias MF Almeida KR De Paula RP Vieira LVF Oliveira PTC Carvalho |
| author_sort | DM Dourado |
| collection | DOAJ |
| description | The present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n = 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 µL of concentrated venom (40 µg/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminum-arsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation. To evaluate the myotoxic activity of the venom, CK activity was measured and the muscle was histologically analyzed. The low-level laser reduced venom-induced CK activity in the groups euthanized at 3 hours, 24 hours and 3 days (p < 0.0001). Histological analysis revealed that low-level laser reduced neutrophilic inflammation as well as myofibrillar edema, hemorrhage and myonecrosis following B. neuwiedi envenomation. These results suggest that low-level laser can be useful as an adjunct therapy following B. neuwiedi envenomation. |
| first_indexed | 2024-12-10T06:30:01Z |
| format | Article |
| id | doaj.art-0a7f0688911241f2b9466d01a2660d89 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-10T06:30:01Z |
| publishDate | 2008-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-0a7f0688911241f2b9466d01a2660d892022-12-22T01:59:05ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992008-01-0114342343410.1590/S1678-91992008000300004The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venomDM DouradoR MatiasMF AlmeidaKR De PaulaRP VieiraLVF OliveiraPTC CarvalhoThe present study aimed to assess the effects of low-level laser (660 nm) on myonecrosis caused by the insertion of Bothrops neuwiedi venom in the gastrocnemius muscle of rats. Male Wistar rats were divided into three groups (n = 24 each): Group S (0.9% saline solution); Group V (venom) and Group VLLL (venom plus low-level laser). These categories were subdivided into four additional groups (n = 6) based on the euthanasia timing (3 hours, 24 hours, 3 days and 7 days). The groups V and VLLL were inoculated with 100 µL of concentrated venom (40 µg/mL) in the gastrocnemius muscle. The muscle was irradiated using a gallium-aluminum-arsenide laser (GaAlAs) at 35 mW power and 4 J/cm² energy density for 3 hours, 24 hours, 3 days or 7 days after venom inoculation. To evaluate the myotoxic activity of the venom, CK activity was measured and the muscle was histologically analyzed. The low-level laser reduced venom-induced CK activity in the groups euthanized at 3 hours, 24 hours and 3 days (p < 0.0001). Histological analysis revealed that low-level laser reduced neutrophilic inflammation as well as myofibrillar edema, hemorrhage and myonecrosis following B. neuwiedi envenomation. These results suggest that low-level laser can be useful as an adjunct therapy following B. neuwiedi envenomation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300004myonecrosis, B. neuwiedi venomlow-level laser |
| spellingShingle | DM Dourado R Matias MF Almeida KR De Paula RP Vieira LVF Oliveira PTC Carvalho The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom Journal of Venomous Animals and Toxins including Tropical Diseases myonecrosis, B. neuwiedi venom low-level laser |
| title | The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom |
| title_full | The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom |
| title_fullStr | The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom |
| title_full_unstemmed | The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom |
| title_short | The effects of low-level laser on muscle damage caused by Bothrops neuwiedi venom |
| title_sort | effects of low level laser on muscle damage caused by bothrops neuwiedi venom |
| topic | myonecrosis, B. neuwiedi venom low-level laser |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300004 |
| work_keys_str_mv | AT dmdourado theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT rmatias theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT mfalmeida theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT krdepaula theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT rpvieira theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT lvfoliveira theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT ptccarvalho theeffectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT dmdourado effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT rmatias effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT mfalmeida effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT krdepaula effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT rpvieira effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT lvfoliveira effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom AT ptccarvalho effectsoflowlevellaseronmuscledamagecausedbybothropsneuwiedivenom |