Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes
The antioxidant and antimicrobial activity of methanol or dichloromethane extracts of O. dictamnus, produced from wild and organic cultivated specimens, were determined. The Rancimat and malondialdehyde (MDA) by HPLC methods were used to measure the antioxidant action, in comparison with that of the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2007-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/12/5/932/ |
| _version_ | 1818011545727139840 |
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| author | John Tsaknis Ioanna Chinou Stavros Lala Olga Gortzi |
| author_facet | John Tsaknis Ioanna Chinou Stavros Lala Olga Gortzi |
| author_sort | John Tsaknis |
| collection | DOAJ |
| description | The antioxidant and antimicrobial activity of methanol or dichloromethane extracts of O. dictamnus, produced from wild and organic cultivated specimens, were determined. The Rancimat and malondialdehyde (MDA) by HPLC methods were used to measure the antioxidant action, in comparison with that of the common commercial antioxidants butylated hydroxytoluene (BHT) and α-tocopherol. The extracts that presented high antioxidant activity were encapsulated in liposomes and their antioxidant action was again estimated using differential scanning calorimetry (DSC). Thermaloxidative decomposition of the samples (pure liposomes and encapsulated extracts) and the modification of the main transition temperature for the lipid mixture and the splitting of the calorimetric peak in the presence of the antioxidants were also studied by the DSC method. All extracts showed antioxidant and antimicrobial activities. Their action proved superior to α-tocopherol. The methanol extract of organic cultivated O. dictamnus (240 ppm) showed higher activity than butylated hydroxytoluene. After encapsulation in liposomes the antioxidant as well as antimicrobial activities proved to be higher than those of the same extracts in pure form. |
| first_indexed | 2024-04-14T06:09:03Z |
| format | Article |
| id | doaj.art-0a7f0fbbf5554eb6abd44cffc06c269c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T06:09:03Z |
| publishDate | 2007-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0a7f0fbbf5554eb6abd44cffc06c269c2022-12-22T02:08:25ZengMDPI AGMolecules1420-30492007-05-0112593294510.3390/12050932Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in LiposomesJohn TsaknisIoanna ChinouStavros LalaOlga GortziThe antioxidant and antimicrobial activity of methanol or dichloromethane extracts of O. dictamnus, produced from wild and organic cultivated specimens, were determined. The Rancimat and malondialdehyde (MDA) by HPLC methods were used to measure the antioxidant action, in comparison with that of the common commercial antioxidants butylated hydroxytoluene (BHT) and α-tocopherol. The extracts that presented high antioxidant activity were encapsulated in liposomes and their antioxidant action was again estimated using differential scanning calorimetry (DSC). Thermaloxidative decomposition of the samples (pure liposomes and encapsulated extracts) and the modification of the main transition temperature for the lipid mixture and the splitting of the calorimetric peak in the presence of the antioxidants were also studied by the DSC method. All extracts showed antioxidant and antimicrobial activities. Their action proved superior to α-tocopherol. The methanol extract of organic cultivated O. dictamnus (240 ppm) showed higher activity than butylated hydroxytoluene. After encapsulation in liposomes the antioxidant as well as antimicrobial activities proved to be higher than those of the same extracts in pure form.http://www.mdpi.com/1420-3049/12/5/932/Origanum dictamnusliposomesantimicrobial activityantioxidant activityDSC. |
| spellingShingle | John Tsaknis Ioanna Chinou Stavros Lala Olga Gortzi Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes Molecules Origanum dictamnus liposomes antimicrobial activity antioxidant activity DSC. |
| title | Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes |
| title_full | Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes |
| title_fullStr | Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes |
| title_full_unstemmed | Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes |
| title_short | Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes |
| title_sort | evaluation of the antimicrobial and antioxidant activities of origanum dictamnus extracts before and after encapsulation in liposomes |
| topic | Origanum dictamnus liposomes antimicrobial activity antioxidant activity DSC. |
| url | http://www.mdpi.com/1420-3049/12/5/932/ |
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