Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419
Fusarium wilt of banana (FWB) is the main threatening factor for banana production worldwide. To explore bacterial biocontrol resources for FWB, the antagonistic effective strains were isolated from banana-producing areas in Yunnan Province, China. Two isolates (YN0904 and YN1419) displaying strong...
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MDPI AG
2021-09-01
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| author | Huacai Fan Shu Li Li Zeng Ping He Shengtao Xu Tingting Bai Yuling Huang Zhixiang Guo Si-Jun Zheng |
| author_facet | Huacai Fan Shu Li Li Zeng Ping He Shengtao Xu Tingting Bai Yuling Huang Zhixiang Guo Si-Jun Zheng |
| author_sort | Huacai Fan |
| collection | DOAJ |
| description | Fusarium wilt of banana (FWB) is the main threatening factor for banana production worldwide. To explore bacterial biocontrol resources for FWB, the antagonistic effective strains were isolated from banana-producing areas in Yunnan Province, China. Two isolates (YN0904 and YN1419) displaying strong antagonism against Tropical Race 4 (TR4) were identified from a total of 813 strains of endophytic bacteria. TR4 inhibition rates of YN0904 and YN1419 were 79.6% and 81.3%, respectively. By looking at morphological, molecular, physiological and biochemical characteristics, YN0904 was identified as <i>Bacillus amyloliquefaciens</i>, while YN1419 was identified as <i>B. subtillis</i>. The control effects of YN0904 and YN1419 on TR4 in greenhouse experiments were 82.6% and 85.6%, respectively. Furthermore, YN0904 obviously promoted the growth of banana plantlets. In addition, biocontrol marker genes related to the biosynthesis of antibiotics synthesized and auxin key synthetase genes could be detected in YN0904. Surprisingly, the marker gene <i>sboA</i> could be exclusively detected in YN1419, while other marker genes were all absent. Molecular characterization results could provide a theoretical basis for expounding the biocontrol mechanisms of these two strains. We concluded that natively antagonistic strains derived from local banana plantations could provide new biological control resources for FWB. |
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| language | English |
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| spelling | doaj.art-0a7fdaff4c974a3aaafd4a38e745fd972023-11-22T18:46:34ZengMDPI AGJournal of Fungi2309-608X2021-09-0171079510.3390/jof7100795Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419Huacai Fan0Shu Li1Li Zeng2Ping He3Shengtao Xu4Tingting Bai5Yuling Huang6Zhixiang Guo7Si-Jun Zheng8Yunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaYunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming 650205, ChinaFusarium wilt of banana (FWB) is the main threatening factor for banana production worldwide. To explore bacterial biocontrol resources for FWB, the antagonistic effective strains were isolated from banana-producing areas in Yunnan Province, China. Two isolates (YN0904 and YN1419) displaying strong antagonism against Tropical Race 4 (TR4) were identified from a total of 813 strains of endophytic bacteria. TR4 inhibition rates of YN0904 and YN1419 were 79.6% and 81.3%, respectively. By looking at morphological, molecular, physiological and biochemical characteristics, YN0904 was identified as <i>Bacillus amyloliquefaciens</i>, while YN1419 was identified as <i>B. subtillis</i>. The control effects of YN0904 and YN1419 on TR4 in greenhouse experiments were 82.6% and 85.6%, respectively. Furthermore, YN0904 obviously promoted the growth of banana plantlets. In addition, biocontrol marker genes related to the biosynthesis of antibiotics synthesized and auxin key synthetase genes could be detected in YN0904. Surprisingly, the marker gene <i>sboA</i> could be exclusively detected in YN1419, while other marker genes were all absent. Molecular characterization results could provide a theoretical basis for expounding the biocontrol mechanisms of these two strains. We concluded that natively antagonistic strains derived from local banana plantations could provide new biological control resources for FWB.https://www.mdpi.com/2309-608X/7/10/795<i>Foc</i> TR4<i>Bacillus amyloliquefaciens</i><i>B. subtillis</i>TR4-inhibitorybiocontrolbiocontrol mechanism |
| spellingShingle | Huacai Fan Shu Li Li Zeng Ping He Shengtao Xu Tingting Bai Yuling Huang Zhixiang Guo Si-Jun Zheng Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 Journal of Fungi <i>Foc</i> TR4 <i>Bacillus amyloliquefaciens</i> <i>B. subtillis</i> TR4-inhibitory biocontrol biocontrol mechanism |
| title | Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 |
| title_full | Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 |
| title_fullStr | Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 |
| title_full_unstemmed | Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 |
| title_short | Biological Control of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using Natively Isolated <i>Bacillus</i> spp. YN0904 and YN1419 |
| title_sort | biological control of i fusarium oxysporum i f sp i cubense i tropical race 4 using natively isolated i bacillus i spp yn0904 and yn1419 |
| topic | <i>Foc</i> TR4 <i>Bacillus amyloliquefaciens</i> <i>B. subtillis</i> TR4-inhibitory biocontrol biocontrol mechanism |
| url | https://www.mdpi.com/2309-608X/7/10/795 |
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