The potential roles of gut microbiome in anal fistula
Abstract Anal fistula is a common proctological disease, but the thorough mechanisms of the anal fistula formation are still unclear. An increasing number of studies have revealed the crucial role of gut microbiota in intestinal diseases. We used 16S rRNA gene sequencing to analyze the intestinal mi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-06-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-023-01560-9 |
| _version_ | 1797806480775184384 |
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| author | Ping Cai Hao Rong Qiaoqiao Zhu Xiaoyu Dai Jianpei Zhao |
| author_facet | Ping Cai Hao Rong Qiaoqiao Zhu Xiaoyu Dai Jianpei Zhao |
| author_sort | Ping Cai |
| collection | DOAJ |
| description | Abstract Anal fistula is a common proctological disease, but the thorough mechanisms of the anal fistula formation are still unclear. An increasing number of studies have revealed the crucial role of gut microbiota in intestinal diseases. We used 16S rRNA gene sequencing to analyze the intestinal microbiome in order to determine whether there are differences in the microbiome between anal fistula patients and healthy individuals. The microbiome samples were extracted by repeatedly wiping the rectal wall with intestinal swab. Before this operation, the whole intestine of all participants was irrigated and the score of the Boston bowel preparation scale reached 9. The biodiversity of gut microbiome of rectum revealed significant difference between anal fistula patients and healthy individuals. 36 discriminative taxa were identified by LEfSe analysis between two groups. At the phylum level, Synergistetes was enriched in anal fistula patients, while Proteobacteria was higher in healthy individuals. We also found that at the genus level, Blautia, Faecalibacterium, Ruminococcus, Coprococcus, Bacteroides, Clostridium, Megamonas and Anaerotruncus were highly enriched in anal fistula patients, while the microbiome of healthy individuals was enriched with Peptoniphilus and Corynebacterium. Spearman correlations showed the extensive and close association among genera and species. Finally, a diagnostic prediction model was constructed by random forest classifier, and the area under curve (AUC) reached 0.990. This study gave an important hint for analyzing gut microbiome of rectum in anal fistula patient. Keypoints. We use the 16S rRNA gene sequencing to test the microbiome samples extracted from the intestinal swab. This is the first study to explore the gut microbiome of rectum using this workflow. We also found the distinct gut microbiome of rectum differences between anal fistula patients and healthy individuals. |
| first_indexed | 2024-03-13T06:07:58Z |
| format | Article |
| id | doaj.art-0a80c8c3bdcd451cb6366e93541ed2fb |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-03-13T06:07:58Z |
| publishDate | 2023-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-0a80c8c3bdcd451cb6366e93541ed2fb2023-06-11T11:26:10ZengSpringerOpenAMB Express2191-08552023-06-011311910.1186/s13568-023-01560-9The potential roles of gut microbiome in anal fistulaPing Cai0Hao Rong1Qiaoqiao Zhu2Xiaoyu Dai3Jianpei Zhao4Ningbo No.2 HospitalNingbo No.2 HospitalNingbo No.2 HospitalNingbo No.2 HospitalNingbo No.2 HospitalAbstract Anal fistula is a common proctological disease, but the thorough mechanisms of the anal fistula formation are still unclear. An increasing number of studies have revealed the crucial role of gut microbiota in intestinal diseases. We used 16S rRNA gene sequencing to analyze the intestinal microbiome in order to determine whether there are differences in the microbiome between anal fistula patients and healthy individuals. The microbiome samples were extracted by repeatedly wiping the rectal wall with intestinal swab. Before this operation, the whole intestine of all participants was irrigated and the score of the Boston bowel preparation scale reached 9. The biodiversity of gut microbiome of rectum revealed significant difference between anal fistula patients and healthy individuals. 36 discriminative taxa were identified by LEfSe analysis between two groups. At the phylum level, Synergistetes was enriched in anal fistula patients, while Proteobacteria was higher in healthy individuals. We also found that at the genus level, Blautia, Faecalibacterium, Ruminococcus, Coprococcus, Bacteroides, Clostridium, Megamonas and Anaerotruncus were highly enriched in anal fistula patients, while the microbiome of healthy individuals was enriched with Peptoniphilus and Corynebacterium. Spearman correlations showed the extensive and close association among genera and species. Finally, a diagnostic prediction model was constructed by random forest classifier, and the area under curve (AUC) reached 0.990. This study gave an important hint for analyzing gut microbiome of rectum in anal fistula patient. Keypoints. We use the 16S rRNA gene sequencing to test the microbiome samples extracted from the intestinal swab. This is the first study to explore the gut microbiome of rectum using this workflow. We also found the distinct gut microbiome of rectum differences between anal fistula patients and healthy individuals.https://doi.org/10.1186/s13568-023-01560-9Anal fistulaGut microbiome16S rRNA gene sequencingClinical research |
| spellingShingle | Ping Cai Hao Rong Qiaoqiao Zhu Xiaoyu Dai Jianpei Zhao The potential roles of gut microbiome in anal fistula AMB Express Anal fistula Gut microbiome 16S rRNA gene sequencing Clinical research |
| title | The potential roles of gut microbiome in anal fistula |
| title_full | The potential roles of gut microbiome in anal fistula |
| title_fullStr | The potential roles of gut microbiome in anal fistula |
| title_full_unstemmed | The potential roles of gut microbiome in anal fistula |
| title_short | The potential roles of gut microbiome in anal fistula |
| title_sort | potential roles of gut microbiome in anal fistula |
| topic | Anal fistula Gut microbiome 16S rRNA gene sequencing Clinical research |
| url | https://doi.org/10.1186/s13568-023-01560-9 |
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