Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin
Soil microorganisms are greatly affected by their microenvironment. To reveal the influence of different land use patterns on the composition and diversity of soil bacterial and fungal communities, this study analyzed microbial (bacteria and fungi) community composition and diversity under different...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/5/1004 |
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| author | Zhe He Chenglin Yuan Peirou Chen Ziqiang Rong Ting Peng Taimoor Hassan Farooq Guangjun Wang Wende Yan Jun Wang |
| author_facet | Zhe He Chenglin Yuan Peirou Chen Ziqiang Rong Ting Peng Taimoor Hassan Farooq Guangjun Wang Wende Yan Jun Wang |
| author_sort | Zhe He |
| collection | DOAJ |
| description | Soil microorganisms are greatly affected by their microenvironment. To reveal the influence of different land use patterns on the composition and diversity of soil bacterial and fungal communities, this study analyzed microbial (bacteria and fungi) community composition and diversity under different land use patterns (vegetable land, wasteland, woodland, cultivated land) based on 16S rRNA, 18S rRNA, and high-throughput sequencing method in the Taojia River Basin. Spearman analysis and redundancy analysis (RDA) were used to explore the correlation between soil physicochemical properties and soil fungal and bacterial community composition, and a partial least squares path model (PLS-PM) was constructed to express the causal relationship between soil physicochemical properties and soil bacterial and fungal community diversity. The results showed that the soil bacterial species richness was highest in vegetable land and the lowest in the wasteland. <i>Proteobacteria</i> is the dominant phylum (20.69%–32.70%), and <i>Actinobacteri</i>a is the dominant class (7.99%–16.95%). The species richness of fungi in woodland was the highest, while was the lowest in cultivated land. The dominant phylum of fungi in vegetable land, woodland, and cultivated land is <i>Mucoromycota</i>, 29.39%, 41.36%, and 22.67%, respectively. <i>Ascomycota</i> (42.16%) is the dominant phylum in wasteland. <i>Sordariomyetes</i> of <i>Ascomycota</i> is the dominant class in wasteland and cultivated land. <i>Mortierellomycetes</i> and <i>Glomeromycetes</i> of <i>Mucoromycota</i> are the dominant class in vegetable land and woodland. The results of the Spearman analysis revealed that the dominant groups in the bacterial and fungal communities had significant correlations with soil pH, clay, and sand (<i>p</i> < 0.01). The RDA results showed that soil clay, pH, and moisture were the key environmental factors affecting the diversity of soil microbial communities. Fungal diversity is more affected by different land use patterns than bacteria. These results provided a theoretical basis for the changes in soil microbial community composition and diversity in river basins. |
| first_indexed | 2024-03-11T03:43:18Z |
| format | Article |
| id | doaj.art-0a8168ee75204a57a3abf3daa1ee6423 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T03:43:18Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-0a8168ee75204a57a3abf3daa1ee64232023-11-18T01:24:52ZengMDPI AGForests1999-49072023-05-01145100410.3390/f14051004Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River BasinZhe He0Chenglin Yuan1Peirou Chen2Ziqiang Rong3Ting Peng4Taimoor Hassan Farooq5Guangjun Wang6Wende Yan7Jun Wang8National Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaBangor College China, A Joint School between Bangor University and Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Engineering Laboratory for Applied Technology in Forestry and Ecology in South China, Central South University of Forestry and Technology, Changsha 410004, ChinaSoil microorganisms are greatly affected by their microenvironment. To reveal the influence of different land use patterns on the composition and diversity of soil bacterial and fungal communities, this study analyzed microbial (bacteria and fungi) community composition and diversity under different land use patterns (vegetable land, wasteland, woodland, cultivated land) based on 16S rRNA, 18S rRNA, and high-throughput sequencing method in the Taojia River Basin. Spearman analysis and redundancy analysis (RDA) were used to explore the correlation between soil physicochemical properties and soil fungal and bacterial community composition, and a partial least squares path model (PLS-PM) was constructed to express the causal relationship between soil physicochemical properties and soil bacterial and fungal community diversity. The results showed that the soil bacterial species richness was highest in vegetable land and the lowest in the wasteland. <i>Proteobacteria</i> is the dominant phylum (20.69%–32.70%), and <i>Actinobacteri</i>a is the dominant class (7.99%–16.95%). The species richness of fungi in woodland was the highest, while was the lowest in cultivated land. The dominant phylum of fungi in vegetable land, woodland, and cultivated land is <i>Mucoromycota</i>, 29.39%, 41.36%, and 22.67%, respectively. <i>Ascomycota</i> (42.16%) is the dominant phylum in wasteland. <i>Sordariomyetes</i> of <i>Ascomycota</i> is the dominant class in wasteland and cultivated land. <i>Mortierellomycetes</i> and <i>Glomeromycetes</i> of <i>Mucoromycota</i> are the dominant class in vegetable land and woodland. The results of the Spearman analysis revealed that the dominant groups in the bacterial and fungal communities had significant correlations with soil pH, clay, and sand (<i>p</i> < 0.01). The RDA results showed that soil clay, pH, and moisture were the key environmental factors affecting the diversity of soil microbial communities. Fungal diversity is more affected by different land use patterns than bacteria. These results provided a theoretical basis for the changes in soil microbial community composition and diversity in river basins.https://www.mdpi.com/1999-4907/14/5/1004Taojia River Basinland usemicrobial community compositiondiversity index |
| spellingShingle | Zhe He Chenglin Yuan Peirou Chen Ziqiang Rong Ting Peng Taimoor Hassan Farooq Guangjun Wang Wende Yan Jun Wang Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin Forests Taojia River Basin land use microbial community composition diversity index |
| title | Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin |
| title_full | Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin |
| title_fullStr | Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin |
| title_full_unstemmed | Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin |
| title_short | Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin |
| title_sort | soil microbial community composition and diversity analysis under different land use patterns in taojia river basin |
| topic | Taojia River Basin land use microbial community composition diversity index |
| url | https://www.mdpi.com/1999-4907/14/5/1004 |
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