Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food
Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes r...
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MDPI AG
2020-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/9/734 |
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| author | Wioleta Chajęcka-Wierzchowska Joanna Gajewska Patryk Wiśniewski Anna Zadernowska |
| author_facet | Wioleta Chajęcka-Wierzchowska Joanna Gajewska Patryk Wiśniewski Anna Zadernowska |
| author_sort | Wioleta Chajęcka-Wierzchowska |
| collection | DOAJ |
| description | Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using polymerase chain reaction (PCR) to five superantigenic toxin genes, including five different types of classical enterotoxins (<i>sea, seb, sec, sed</i> and <i>see</i>) and the toxic shock syndrome toxin-1 (<i>tsst</i>-1) as well as to supertoxin-like genes. PCR-positive isolates were then tested using immunoenzymatic methods (SET-RPLA, Vidas SET 2) for toxin expression. Out of 118 CoNS strains, the presence of staphylococcal enterotoxins was confirmed in 72% of them. The most frequently found enterotoxin-like genotype was <i>ser</i>, <i>selu</i>. Two of the tested strains had up to ten different enterotoxin genes in the genome at the same time. Although no production of enterotoxins was detected in the CoNS, which means that their possible role in the epidemiology of food-borne diseases is minimal, the data demonstrated that the toxigenic capacity of the CoNS should not be ignored, and that this group of microorganisms should be continuously monitored in food. |
| first_indexed | 2024-03-10T16:32:27Z |
| format | Article |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T16:32:27Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-0a83879595434ac595212cbd46d2c08b2023-11-20T12:44:10ZengMDPI AGPathogens2076-08172020-09-019973410.3390/pathogens9090734Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat FoodWioleta Chajęcka-Wierzchowska0Joanna Gajewska1Patryk Wiśniewski2Anna Zadernowska3Department of Industrial and Food Microbiology, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn, PolandAlthough coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using polymerase chain reaction (PCR) to five superantigenic toxin genes, including five different types of classical enterotoxins (<i>sea, seb, sec, sed</i> and <i>see</i>) and the toxic shock syndrome toxin-1 (<i>tsst</i>-1) as well as to supertoxin-like genes. PCR-positive isolates were then tested using immunoenzymatic methods (SET-RPLA, Vidas SET 2) for toxin expression. Out of 118 CoNS strains, the presence of staphylococcal enterotoxins was confirmed in 72% of them. The most frequently found enterotoxin-like genotype was <i>ser</i>, <i>selu</i>. Two of the tested strains had up to ten different enterotoxin genes in the genome at the same time. Although no production of enterotoxins was detected in the CoNS, which means that their possible role in the epidemiology of food-borne diseases is minimal, the data demonstrated that the toxigenic capacity of the CoNS should not be ignored, and that this group of microorganisms should be continuously monitored in food.https://www.mdpi.com/2076-0817/9/9/734coagulase-negative staphylococciready-to-eat foodstaphylococcal enterotoxins |
| spellingShingle | Wioleta Chajęcka-Wierzchowska Joanna Gajewska Patryk Wiśniewski Anna Zadernowska Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food Pathogens coagulase-negative staphylococci ready-to-eat food staphylococcal enterotoxins |
| title | Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food |
| title_full | Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food |
| title_fullStr | Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food |
| title_full_unstemmed | Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food |
| title_short | Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food |
| title_sort | enterotoxigenic potential of coagulase negative staphylococci from ready to eat food |
| topic | coagulase-negative staphylococci ready-to-eat food staphylococcal enterotoxins |
| url | https://www.mdpi.com/2076-0817/9/9/734 |
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