Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine
The emerging field of nanomedicine provides new approaches for the diagnosis and treatment of diseases, for symptom relief and for monitoring of disease progression. One route of realizing this approach is through carefully constructed nanoparticles. Due to the small size inherent to the nanoparticl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/1/17 |
| _version_ | 1818082331663007744 |
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| author | Alexander Boreham Robert Brodwolf Karolina Walker Rainer Haag Ulrike Alexiev |
| author_facet | Alexander Boreham Robert Brodwolf Karolina Walker Rainer Haag Ulrike Alexiev |
| author_sort | Alexander Boreham |
| collection | DOAJ |
| description | The emerging field of nanomedicine provides new approaches for the diagnosis and treatment of diseases, for symptom relief and for monitoring of disease progression. One route of realizing this approach is through carefully constructed nanoparticles. Due to the small size inherent to the nanoparticles a proper characterization is not trivial. This review highlights the application of time-resolved fluorescence spectroscopy and fluorescence lifetime imaging microscopy (FLIM) for the analysis of nanoparticles, covering aspects ranging from molecular properties to particle detection in tissue samples. The latter technique is particularly important as FLIM allows for distinguishing of target molecules from the autofluorescent background and, due to the environmental sensitivity of the fluorescence lifetime, also offers insights into the local environment of the nanoparticle or its interactions with other biomolecules. Thus, these techniques offer highly suitable tools in the fields of particle development, such as organic chemistry, and in the fields of particle application, such as in experimental dermatology or pharmaceutical research. |
| first_indexed | 2024-12-10T19:20:24Z |
| format | Article |
| id | doaj.art-0a869da8c91f40498e4af5ff488acb18 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T19:20:24Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0a869da8c91f40498e4af5ff488acb182022-12-22T01:36:29ZengMDPI AGMolecules1420-30492016-12-012211710.3390/molecules22010017molecules22010017Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in NanomedicineAlexander Boreham0Robert Brodwolf1Karolina Walker2Rainer Haag3Ulrike Alexiev4Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyInstitut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyInstitut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, GermanyInstitut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, GermanyInstitut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyThe emerging field of nanomedicine provides new approaches for the diagnosis and treatment of diseases, for symptom relief and for monitoring of disease progression. One route of realizing this approach is through carefully constructed nanoparticles. Due to the small size inherent to the nanoparticles a proper characterization is not trivial. This review highlights the application of time-resolved fluorescence spectroscopy and fluorescence lifetime imaging microscopy (FLIM) for the analysis of nanoparticles, covering aspects ranging from molecular properties to particle detection in tissue samples. The latter technique is particularly important as FLIM allows for distinguishing of target molecules from the autofluorescent background and, due to the environmental sensitivity of the fluorescence lifetime, also offers insights into the local environment of the nanoparticle or its interactions with other biomolecules. Thus, these techniques offer highly suitable tools in the fields of particle development, such as organic chemistry, and in the fields of particle application, such as in experimental dermatology or pharmaceutical research.http://www.mdpi.com/1420-3049/22/1/17nanomedicinedendritic nanoparticlestime-resolved fluorescence depolarizationfluorescence lifetime imaging microscopyFLIM |
| spellingShingle | Alexander Boreham Robert Brodwolf Karolina Walker Rainer Haag Ulrike Alexiev Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine Molecules nanomedicine dendritic nanoparticles time-resolved fluorescence depolarization fluorescence lifetime imaging microscopy FLIM |
| title | Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine |
| title_full | Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine |
| title_fullStr | Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine |
| title_full_unstemmed | Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine |
| title_short | Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine |
| title_sort | time resolved fluorescence spectroscopy and fluorescence lifetime imaging microscopy for characterization of dendritic polymer nanoparticles and applications in nanomedicine |
| topic | nanomedicine dendritic nanoparticles time-resolved fluorescence depolarization fluorescence lifetime imaging microscopy FLIM |
| url | http://www.mdpi.com/1420-3049/22/1/17 |
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