L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i>
Biotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (<i>Phragm...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Resources |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-9276/9/7/89 |
| _version_ | 1797561851980021760 |
|---|---|
| author | Linda Schroedter Roland Schneider Lisa Remus Joachim Venus |
| author_facet | Linda Schroedter Roland Schneider Lisa Remus Joachim Venus |
| author_sort | Linda Schroedter |
| collection | DOAJ |
| description | Biotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (<i>Phragmites australis</i>) is a powerfully reproducing sweet grass which grows in wetlands and creates vast monocultural populations. This lignocellulose biomass bears the possibility to be refined to value-added products, without competing with agro industrial land. Besides utilizing reed as a renewable and inexpensive substrate, low-cost nutritional supplementation was analyzed for the fermentation of thermophilic <i>Bacillus</i> <i>coagulans.</i> Various nutritional sources such as baker’s and brewer’s yeast, lucerne green juice and tryptone were investigated for the replacement of yeast extract. The structure of the lignocellulosic material was tackled by chemical treatment (1% NaOH) and enzymatic hydrolysis (Cellic<sup>®</sup> CTec2). <i>B.</i> <i>coagulans</i> DSM ID 14-300 was employed for the homofermentative conversion of the released hexose and pentose sugars to polymerizable L-(+)-LA of over 99.5% optical purity. The addition of autolyzed baker’s yeast led to the best results of fermentation, enabling an LA titer of 28.3 g L<sup>−1</sup> and a yield of 91.6%. |
| first_indexed | 2024-03-10T18:20:28Z |
| format | Article |
| id | doaj.art-0a86ea20fb91414dad7559249ccb6dd8 |
| institution | Directory Open Access Journal |
| issn | 2079-9276 |
| language | English |
| last_indexed | 2024-03-10T18:20:28Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Resources |
| spelling | doaj.art-0a86ea20fb91414dad7559249ccb6dd82023-11-20T07:21:59ZengMDPI AGResources2079-92762020-07-01978910.3390/resources9070089L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i>Linda Schroedter0Roland Schneider1Lisa Remus2Joachim Venus3Bioengineering Department, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Max-Eyth-Allee 100, 14469 Potsdam, GermanyBioengineering Department, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Max-Eyth-Allee 100, 14469 Potsdam, GermanyInstitute for Biochemistry and Biology (IBB), Faculty of Science, University of Potsdam (UP), Am Neuen Palais 10, 14469 Potsdam, GermanyBioengineering Department, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Max-Eyth-Allee 100, 14469 Potsdam, GermanyBiotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (<i>Phragmites australis</i>) is a powerfully reproducing sweet grass which grows in wetlands and creates vast monocultural populations. This lignocellulose biomass bears the possibility to be refined to value-added products, without competing with agro industrial land. Besides utilizing reed as a renewable and inexpensive substrate, low-cost nutritional supplementation was analyzed for the fermentation of thermophilic <i>Bacillus</i> <i>coagulans.</i> Various nutritional sources such as baker’s and brewer’s yeast, lucerne green juice and tryptone were investigated for the replacement of yeast extract. The structure of the lignocellulosic material was tackled by chemical treatment (1% NaOH) and enzymatic hydrolysis (Cellic<sup>®</sup> CTec2). <i>B.</i> <i>coagulans</i> DSM ID 14-300 was employed for the homofermentative conversion of the released hexose and pentose sugars to polymerizable L-(+)-LA of over 99.5% optical purity. The addition of autolyzed baker’s yeast led to the best results of fermentation, enabling an LA titer of 28.3 g L<sup>−1</sup> and a yield of 91.6%.https://www.mdpi.com/2079-9276/9/7/89lignocellulosereed<i>Phragmites australis</i>lactic acid<i>Bacillus coagulans</i> |
| spellingShingle | Linda Schroedter Roland Schneider Lisa Remus Joachim Venus L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> Resources lignocellulose reed <i>Phragmites australis</i> lactic acid <i>Bacillus coagulans</i> |
| title | L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> |
| title_full | L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> |
| title_fullStr | L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> |
| title_full_unstemmed | L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> |
| title_short | L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of <i>B. coagulans</i> |
| title_sort | l lactic acid from reed comparing various resources for the nutrient provision of i b coagulans i |
| topic | lignocellulose reed <i>Phragmites australis</i> lactic acid <i>Bacillus coagulans</i> |
| url | https://www.mdpi.com/2079-9276/9/7/89 |
| work_keys_str_mv | AT lindaschroedter llacticacidfromreedcomparingvariousresourcesforthenutrientprovisionofibcoagulansi AT rolandschneider llacticacidfromreedcomparingvariousresourcesforthenutrientprovisionofibcoagulansi AT lisaremus llacticacidfromreedcomparingvariousresourcesforthenutrientprovisionofibcoagulansi AT joachimvenus llacticacidfromreedcomparingvariousresourcesforthenutrientprovisionofibcoagulansi |