The microbial community of the cystic fibrosis airway is disrupted in early life.
Molecular techniques have uncovered vast numbers of organisms in the cystic fibrosis (CF) airways, the clinical significance of which is yet to be determined. The aim of this study was to describe and compare the microbial communities of the lower airway of clinically stable children with CF and chi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4272276?pdf=render |
| _version_ | 1818325666591932416 |
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| author | Julie Renwick Paul McNally Bettina John Todd DeSantis Barry Linnane Philip Murphy SHIELD CF |
| author_facet | Julie Renwick Paul McNally Bettina John Todd DeSantis Barry Linnane Philip Murphy SHIELD CF |
| author_sort | Julie Renwick |
| collection | DOAJ |
| description | Molecular techniques have uncovered vast numbers of organisms in the cystic fibrosis (CF) airways, the clinical significance of which is yet to be determined. The aim of this study was to describe and compare the microbial communities of the lower airway of clinically stable children with CF and children without CF.Bronchoalveolar lavage (BAL) fluid and paired oropharyngeal swabs from clinically stable children with CF (n = 13) and BAL from children without CF (n = 9) were collected. DNA was isolated, the 16S rRNA regions amplified, fragmented, biotinylated and hybridised to a 16S rRNA microarray. Patient medical and demographic information was recorded and standard microbiological culture was performed.A diverse bacterial community was detected in the lower airways of children with CF and children without CF. The airway microbiome of clinically stable children with CF and children without CF were significantly different as measured by Shannon's Diversity Indices (p = 0.001; t test) and Principle coordinate analysis (p = 0.01; Adonis test). Overall the CF airway microbial community was more variable and had a less even distribution than the microbial community in the airways of children without CF. We highlighted several bacteria of interest, particularly Prevotella veroralis, CW040 and a Corynebacterium, which were of significantly differential abundance between the CF and non-CF lower airways. Both Pseudomonas aeruginosa and Streptococcus pneumoniae culture abundance were found to be associated with CF airway microbial community structure. The CF upper and lower airways were found to have a broadly similar microbial milieu.The microbial communities in the lower airways of stable children with CF and children without CF show significant differences in overall diversity. These discrepancies indicate a disruption of the airway microflora occurring early in life in children with CF. |
| first_indexed | 2024-12-13T11:48:07Z |
| format | Article |
| id | doaj.art-0a8af26aa33347aba8dd46aa07576a42 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T11:48:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0a8af26aa33347aba8dd46aa07576a422022-12-21T23:47:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e10979810.1371/journal.pone.0109798The microbial community of the cystic fibrosis airway is disrupted in early life.Julie RenwickPaul McNallyBettina JohnTodd DeSantisBarry LinnanePhilip MurphySHIELD CFMolecular techniques have uncovered vast numbers of organisms in the cystic fibrosis (CF) airways, the clinical significance of which is yet to be determined. The aim of this study was to describe and compare the microbial communities of the lower airway of clinically stable children with CF and children without CF.Bronchoalveolar lavage (BAL) fluid and paired oropharyngeal swabs from clinically stable children with CF (n = 13) and BAL from children without CF (n = 9) were collected. DNA was isolated, the 16S rRNA regions amplified, fragmented, biotinylated and hybridised to a 16S rRNA microarray. Patient medical and demographic information was recorded and standard microbiological culture was performed.A diverse bacterial community was detected in the lower airways of children with CF and children without CF. The airway microbiome of clinically stable children with CF and children without CF were significantly different as measured by Shannon's Diversity Indices (p = 0.001; t test) and Principle coordinate analysis (p = 0.01; Adonis test). Overall the CF airway microbial community was more variable and had a less even distribution than the microbial community in the airways of children without CF. We highlighted several bacteria of interest, particularly Prevotella veroralis, CW040 and a Corynebacterium, which were of significantly differential abundance between the CF and non-CF lower airways. Both Pseudomonas aeruginosa and Streptococcus pneumoniae culture abundance were found to be associated with CF airway microbial community structure. The CF upper and lower airways were found to have a broadly similar microbial milieu.The microbial communities in the lower airways of stable children with CF and children without CF show significant differences in overall diversity. These discrepancies indicate a disruption of the airway microflora occurring early in life in children with CF.http://europepmc.org/articles/PMC4272276?pdf=render |
| spellingShingle | Julie Renwick Paul McNally Bettina John Todd DeSantis Barry Linnane Philip Murphy SHIELD CF The microbial community of the cystic fibrosis airway is disrupted in early life. PLoS ONE |
| title | The microbial community of the cystic fibrosis airway is disrupted in early life. |
| title_full | The microbial community of the cystic fibrosis airway is disrupted in early life. |
| title_fullStr | The microbial community of the cystic fibrosis airway is disrupted in early life. |
| title_full_unstemmed | The microbial community of the cystic fibrosis airway is disrupted in early life. |
| title_short | The microbial community of the cystic fibrosis airway is disrupted in early life. |
| title_sort | microbial community of the cystic fibrosis airway is disrupted in early life |
| url | http://europepmc.org/articles/PMC4272276?pdf=render |
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