Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease
Abstract Background Coronary Heart Disease (CHD) is the leading cause of death in industrialized countries. There is currently no direct relation between CHD and type 2 diabetes mellitus (T2D), one of the major modifiable risk factors for CHD. This study was carried out for genes expression profilin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Diabetology & Metabolic Syndrome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13098-022-00878-x |
| _version_ | 1811222964744486912 |
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| author | Salma Ahmadloo King-Hwa Ling Ahmad Fazli Ghazaleh Larijani Nooshin Ghodsian Sanaz Mohammadi Naser Amini Vahid Hosseinpour Sarmadi Patimah Ismail |
| author_facet | Salma Ahmadloo King-Hwa Ling Ahmad Fazli Ghazaleh Larijani Nooshin Ghodsian Sanaz Mohammadi Naser Amini Vahid Hosseinpour Sarmadi Patimah Ismail |
| author_sort | Salma Ahmadloo |
| collection | DOAJ |
| description | Abstract Background Coronary Heart Disease (CHD) is the leading cause of death in industrialized countries. There is currently no direct relation between CHD and type 2 diabetes mellitus (T2D), one of the major modifiable risk factors for CHD. This study was carried out for genes expression profiling of T2D associated genes to identify related biological processes/es and modulated signaling pathway/s of male subjects with CHD. Method the subjects were divided into four groups based on their disease, including control, type 2 diabetes mellitus (T2D), CHD, and CHD + T2D groups. The RNA was extracted from their blood, and RT2 Profiler™ PCR Array was utilized to determine gene profiling between groups. Finally, the PCR Array results were validated by using Q-RT-PCR in a more extensive and independent population. Result PCR Array results revealed that the T2D and T2D + CHD groups shared 11 genes significantly up-regulated in both groups. Further analysis showed that the mRNA levels of AKT2, IL12B, IL6, IRS1, IRS2, MAPK14, and NFKB1 increased. Consequently, the mRNA levels of AQP2, FOXP3, G6PD, and PIK3R1 declined in the T2D + CHD group compared to the T2D group. Furthermore, in silico analysis indicated 36 Gene Ontology terms and 59 signaling pathways were significantly enriched in both groups, which may be a culprit in susceptibility of diabetic patients to CHD development. Conclusion Finally, the results revealed six genes as a hub gene in altering various biological processes and signaling pathways. The expression trend of these identified genes might be used as potential markers and diagnostic tools for the early identification of the vulnerability of T2D patients to develop premature CHD. Graphical Abstract |
| first_indexed | 2024-04-12T08:23:56Z |
| format | Article |
| id | doaj.art-0a8b1a2db7d343e18296bc51d2ad9fbe |
| institution | Directory Open Access Journal |
| issn | 1758-5996 |
| language | English |
| last_indexed | 2024-04-12T08:23:56Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Diabetology & Metabolic Syndrome |
| spelling | doaj.art-0a8b1a2db7d343e18296bc51d2ad9fbe2022-12-22T03:40:27ZengBMCDiabetology & Metabolic Syndrome1758-59962022-07-0114111510.1186/s13098-022-00878-xSignature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart diseaseSalma Ahmadloo0King-Hwa Ling1Ahmad Fazli2Ghazaleh Larijani3Nooshin Ghodsian4Sanaz Mohammadi5Naser Amini6Vahid Hosseinpour Sarmadi7Patimah Ismail8Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Biology, Science and Research Branch, Islamic Azad UniversityDepartment of Biomedical Engineering, University of British ColumbiaFaculty of Biological Science and Technology, Shahid Beheshti UniversityCellular and Molecular Research Center, Iran University of Medical SciencesCellular and Molecular Research Center, Iran University of Medical SciencesDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaAbstract Background Coronary Heart Disease (CHD) is the leading cause of death in industrialized countries. There is currently no direct relation between CHD and type 2 diabetes mellitus (T2D), one of the major modifiable risk factors for CHD. This study was carried out for genes expression profiling of T2D associated genes to identify related biological processes/es and modulated signaling pathway/s of male subjects with CHD. Method the subjects were divided into four groups based on their disease, including control, type 2 diabetes mellitus (T2D), CHD, and CHD + T2D groups. The RNA was extracted from their blood, and RT2 Profiler™ PCR Array was utilized to determine gene profiling between groups. Finally, the PCR Array results were validated by using Q-RT-PCR in a more extensive and independent population. Result PCR Array results revealed that the T2D and T2D + CHD groups shared 11 genes significantly up-regulated in both groups. Further analysis showed that the mRNA levels of AKT2, IL12B, IL6, IRS1, IRS2, MAPK14, and NFKB1 increased. Consequently, the mRNA levels of AQP2, FOXP3, G6PD, and PIK3R1 declined in the T2D + CHD group compared to the T2D group. Furthermore, in silico analysis indicated 36 Gene Ontology terms and 59 signaling pathways were significantly enriched in both groups, which may be a culprit in susceptibility of diabetic patients to CHD development. Conclusion Finally, the results revealed six genes as a hub gene in altering various biological processes and signaling pathways. The expression trend of these identified genes might be used as potential markers and diagnostic tools for the early identification of the vulnerability of T2D patients to develop premature CHD. Graphical Abstracthttps://doi.org/10.1186/s13098-022-00878-xCoronary heart diseaseType 2 diabetes mellitusGene expressionSignaling pathway |
| spellingShingle | Salma Ahmadloo King-Hwa Ling Ahmad Fazli Ghazaleh Larijani Nooshin Ghodsian Sanaz Mohammadi Naser Amini Vahid Hosseinpour Sarmadi Patimah Ismail Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease Diabetology & Metabolic Syndrome Coronary heart disease Type 2 diabetes mellitus Gene expression Signaling pathway |
| title | Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| title_full | Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| title_fullStr | Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| title_full_unstemmed | Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| title_short | Signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| title_sort | signature pattern of gene expression and signaling pathway in premature diabetic patients uncover their correlation to early age coronary heart disease |
| topic | Coronary heart disease Type 2 diabetes mellitus Gene expression Signaling pathway |
| url | https://doi.org/10.1186/s13098-022-00878-x |
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