Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept
Monitoring measurable residual disease (MRD) is crucial to assess treatment response in Multiple Myeloma (MM). Detection of MRD in peripheral blood (PB) by exploring Extracellular Vesicles (EVs), and their cargo, would allow frequent and minimally invasive monitoring of MM. This work aims to detect...
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| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13686 |
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| author | Rui Bergantim Sara Peixoto da Silva Bárbara Polónia Mélanie A. G. Barbosa André Albergaria Jorge Lima Hugo R. Caires José E. Guimarães M. Helena Vasconcelos |
| author_facet | Rui Bergantim Sara Peixoto da Silva Bárbara Polónia Mélanie A. G. Barbosa André Albergaria Jorge Lima Hugo R. Caires José E. Guimarães M. Helena Vasconcelos |
| author_sort | Rui Bergantim |
| collection | DOAJ |
| description | Monitoring measurable residual disease (MRD) is crucial to assess treatment response in Multiple Myeloma (MM). Detection of MRD in peripheral blood (PB) by exploring Extracellular Vesicles (EVs), and their cargo, would allow frequent and minimally invasive monitoring of MM. This work aims to detect biomarkers of MRD in EVs isolated from MM patient samples at diagnosis and remission and compare the MRD-associated content between BM and PB EVs. EVs were isolated by size-exclusion chromatography, concentrated by ultrafiltration, and characterized according to their size and concentration, morphology, protein concentration, and the presence of EV-associated protein markers. EVs from healthy blood donors were used as controls. It was possible to isolate EVs from PB and BM carrying MM markers. Diagnostic samples had different levels of MM markers between PB and BM paired samples, but no differences between PB and BM were found at remission. EVs concentration was lower in the PB of healthy controls than of patients, and MM markers were mostly not detected in EVs from controls. This study pinpoints the potential of PB EVs from MM remission patients as a source of MM biomarkers and as a non-invasive approach for monitoring MRD. |
| first_indexed | 2024-03-09T18:18:15Z |
| format | Article |
| id | doaj.art-0a8b79b3c6be42ddb5a09a8a41fafbdf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:18:15Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0a8b79b3c6be42ddb5a09a8a41fafbdf2023-11-24T08:31:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221368610.3390/ijms232213686Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of ConceptRui Bergantim0Sara Peixoto da Silva1Bárbara Polónia2Mélanie A. G. Barbosa3André Albergaria4Jorge Lima5Hugo R. Caires6José E. Guimarães7M. Helena Vasconcelos8i3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, PortugalMonitoring measurable residual disease (MRD) is crucial to assess treatment response in Multiple Myeloma (MM). Detection of MRD in peripheral blood (PB) by exploring Extracellular Vesicles (EVs), and their cargo, would allow frequent and minimally invasive monitoring of MM. This work aims to detect biomarkers of MRD in EVs isolated from MM patient samples at diagnosis and remission and compare the MRD-associated content between BM and PB EVs. EVs were isolated by size-exclusion chromatography, concentrated by ultrafiltration, and characterized according to their size and concentration, morphology, protein concentration, and the presence of EV-associated protein markers. EVs from healthy blood donors were used as controls. It was possible to isolate EVs from PB and BM carrying MM markers. Diagnostic samples had different levels of MM markers between PB and BM paired samples, but no differences between PB and BM were found at remission. EVs concentration was lower in the PB of healthy controls than of patients, and MM markers were mostly not detected in EVs from controls. This study pinpoints the potential of PB EVs from MM remission patients as a source of MM biomarkers and as a non-invasive approach for monitoring MRD.https://www.mdpi.com/1422-0067/23/22/13686multiple myelomaextracellular vesiclesmeasurable residual diseaseliquid biopsy |
| spellingShingle | Rui Bergantim Sara Peixoto da Silva Bárbara Polónia Mélanie A. G. Barbosa André Albergaria Jorge Lima Hugo R. Caires José E. Guimarães M. Helena Vasconcelos Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept International Journal of Molecular Sciences multiple myeloma extracellular vesicles measurable residual disease liquid biopsy |
| title | Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept |
| title_full | Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept |
| title_fullStr | Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept |
| title_full_unstemmed | Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept |
| title_short | Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from Liquid Biopsies of Multiple Myeloma Patients—A Proof of Concept |
| title_sort | detection of measurable residual disease biomarkers in extracellular vesicles from liquid biopsies of multiple myeloma patients a proof of concept |
| topic | multiple myeloma extracellular vesicles measurable residual disease liquid biopsy |
| url | https://www.mdpi.com/1422-0067/23/22/13686 |
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