Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome
Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production...
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MDPI AG
2022-08-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/9/1347 |
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| author | Mónica Carvalheira Catarina L. Amorim Ana Catarina Oliveira Eliana C. Guarda Eunice Costa Margarida Ribau Teixeira Paula M. L. Castro Anouk F. Duque Maria A. M. Reis |
| author_facet | Mónica Carvalheira Catarina L. Amorim Ana Catarina Oliveira Eliana C. Guarda Eunice Costa Margarida Ribau Teixeira Paula M. L. Castro Anouk F. Duque Maria A. M. Reis |
| author_sort | Mónica Carvalheira |
| collection | DOAJ |
| description | Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the <i>Meganema</i>, <i>Carnobacterium</i>, <i>Leucobacter</i>, and <i>Paracocccus</i> genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 Cmmol<sub>PHA</sub>/Cmmol<sub>VFA</sub> in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products. |
| first_indexed | 2024-03-09T23:23:04Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:23:04Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-0a8c7292a17c4e6ba8d7ed764cfeef162023-11-23T17:22:35ZengMDPI AGLife2075-17292022-08-01129134710.3390/life12091347Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized MicrobiomeMónica Carvalheira0Catarina L. Amorim1Ana Catarina Oliveira2Eliana C. Guarda3Eunice Costa4Margarida Ribau Teixeira5Paula M. L. Castro6Anouk F. Duque7Maria A. M. Reis8Associate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalUniversidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalUniversidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCENSE—Center for Environmental and Sustainability Research & CHANGE—Global Change and Sustainability Institute, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, PortugalUniversidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, PortugalRaw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the <i>Meganema</i>, <i>Carnobacterium</i>, <i>Leucobacter</i>, and <i>Paracocccus</i> genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 Cmmol<sub>PHA</sub>/Cmmol<sub>VFA</sub> in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.https://www.mdpi.com/2075-1729/12/9/1347biopolymerscircular economywaste valorizationmicrobial community |
| spellingShingle | Mónica Carvalheira Catarina L. Amorim Ana Catarina Oliveira Eliana C. Guarda Eunice Costa Margarida Ribau Teixeira Paula M. L. Castro Anouk F. Duque Maria A. M. Reis Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome Life biopolymers circular economy waste valorization microbial community |
| title | Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome |
| title_full | Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome |
| title_fullStr | Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome |
| title_full_unstemmed | Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome |
| title_short | Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome |
| title_sort | valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiome |
| topic | biopolymers circular economy waste valorization microbial community |
| url | https://www.mdpi.com/2075-1729/12/9/1347 |
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