A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains
Oligodendrocytes (OL) are myelin-forming glial cells in the central nervous system. In vitro primary OL culture models offer the benefit of a more readily controlled environment that facilitates the examination of diverse OL stages and their intricate dynamics. Although conventional methods for prim...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024053908 |
| _version_ | 1797203004596682752 |
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| author | Hanki Kim Bum Jun Kim Seungyon Koh Hyo Jin Cho Xuelian Jin Byung Gon Kim Jun Young Choi |
| author_facet | Hanki Kim Bum Jun Kim Seungyon Koh Hyo Jin Cho Xuelian Jin Byung Gon Kim Jun Young Choi |
| author_sort | Hanki Kim |
| collection | DOAJ |
| description | Oligodendrocytes (OL) are myelin-forming glial cells in the central nervous system. In vitro primary OL culture models offer the benefit of a more readily controlled environment that facilitates the examination of diverse OL stages and their intricate dynamics. Although conventional methods for primary OL culture exist, their performance in terms of simplicity and efficiency can be improved. Here, we introduce a novel method for primary OL culture, namely the E3 (easy, efficient, and effective) method, which greatly improves the simplicity and efficiency of the primary OL culture procedure using neonatal rodent brains. We also provided the optimal media composition for the augmentation of oligodendrocyte progenitor cell (OPC) proliferation and more robust maturation into myelin-forming OLs. Overall, E3 offers an undemanding method for obtaining primary OLs with high yield and quality. Alongside its value as a practical tool, in vitro characteristics of the OL lineage additionally identified during the development of the E3 method have implications for advancing research on OL physiology and pathophysiology. |
| first_indexed | 2024-04-24T08:12:26Z |
| format | Article |
| id | doaj.art-0a8ed1a5687c44dbb73c0add89be97e9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T08:12:26Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0a8ed1a5687c44dbb73c0add89be97e92024-04-17T04:49:29ZengElsevierHeliyon2405-84402024-04-01108e29359A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brainsHanki Kim0Bum Jun Kim1Seungyon Koh2Hyo Jin Cho3Xuelian Jin4Byung Gon Kim5Jun Young Choi6Department of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon, 16499, South KoreaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon, 16499, South KoreaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon, 16499, South Korea; Department of Neurology, Ajou University School of Medicine, Suwon, 16499, South KoreaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South KoreaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Geriatrics Department, The Affiliated Suqian First People's Hospital of Nanjing Medical University, Suqian, 223800, ChinaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Department of Neurology, Ajou University School of Medicine, Suwon, 16499, South KoreaDepartment of Brain Science, Ajou University School of Medicine, Suwon, 16499, South Korea; Department of Neurology, Ajou University School of Medicine, Suwon, 16499, South Korea; Corresponding author. Departments of Brain Science and Neurology, Ajou University School of Medicine, Suwon, 16499, South Korea.Oligodendrocytes (OL) are myelin-forming glial cells in the central nervous system. In vitro primary OL culture models offer the benefit of a more readily controlled environment that facilitates the examination of diverse OL stages and their intricate dynamics. Although conventional methods for primary OL culture exist, their performance in terms of simplicity and efficiency can be improved. Here, we introduce a novel method for primary OL culture, namely the E3 (easy, efficient, and effective) method, which greatly improves the simplicity and efficiency of the primary OL culture procedure using neonatal rodent brains. We also provided the optimal media composition for the augmentation of oligodendrocyte progenitor cell (OPC) proliferation and more robust maturation into myelin-forming OLs. Overall, E3 offers an undemanding method for obtaining primary OLs with high yield and quality. Alongside its value as a practical tool, in vitro characteristics of the OL lineage additionally identified during the development of the E3 method have implications for advancing research on OL physiology and pathophysiology.http://www.sciencedirect.com/science/article/pii/S2405844024053908 |
| spellingShingle | Hanki Kim Bum Jun Kim Seungyon Koh Hyo Jin Cho Xuelian Jin Byung Gon Kim Jun Young Choi A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains Heliyon |
| title | A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| title_full | A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| title_fullStr | A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| title_full_unstemmed | A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| title_short | A primary culture method for the easy, efficient, and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| title_sort | primary culture method for the easy efficient and effective acquisition of oligodendrocyte lineage cells from neonatal rodent brains |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024053908 |
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