Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
The membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification a...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.684227/full |
| _version_ | 1818994473096445952 |
|---|---|
| author | Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei |
| author_facet | Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei |
| author_sort | Pengyun Chen |
| collection | DOAJ |
| description | The membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification and partial functional verification of the members of the MACPF family. Totally, 100 putative MACPF proteins containing complete MACPF domain were identified from the four cotton species. They were classified into three phylogenetic groups and underwent multifold pressure indicating that selection produced new functional differentiation. Cotton MACPF gene family members expanded mainly through the whole-genome duplication (WGD)/segmental followed by the dispersed. Expression and cis-acting elements analysis revealed that MACPFs play a role in resistance to abiotic stresses, and some selected GhMACPFs were able to respond to the PEG and cold stresses. Co-expression analysis showed that GhMACPFs might interact with valine-glutamine (VQ), WRKY, and Apetala 2 (AP2)/ethylene responsive factor (ERF) domain-containing genes under cold stress. In addition, silencing endogenous GhMACPF26 in cotton by the virus-induced gene silencing (VIGS) method indicated that GhMACPF26 negatively regulates cold tolerance. Our data provided a comprehensive phylogenetic evolutionary view of Gossypium MACPFs. The MACPFs may work together with multiple transcriptional factors and play roles in acclimation to abiotic stress, especially cold stress in cotton. |
| first_indexed | 2024-12-20T20:58:30Z |
| format | Article |
| id | doaj.art-0a903bec74d545249d9316189382bff2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T20:58:30Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0a903bec74d545249d9316189382bff22022-12-21T19:26:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-11-011210.3389/fpls.2021.684227684227Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold StressPengyun Chen0Hongliang Jian1Fei Wei2Lijiao Gu3Tingli Hu4Xiaoyan Lv5Xiaohao Guo6Jianhua Lu7Liang Ma8Hantao Wang9Aimin Wu10Guangzhi Mao11Shuxun Yu12Hengling Wei13State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaCollege of Life Sciences, Xinyang Normal University, Xinyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, ChinaThe membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification and partial functional verification of the members of the MACPF family. Totally, 100 putative MACPF proteins containing complete MACPF domain were identified from the four cotton species. They were classified into three phylogenetic groups and underwent multifold pressure indicating that selection produced new functional differentiation. Cotton MACPF gene family members expanded mainly through the whole-genome duplication (WGD)/segmental followed by the dispersed. Expression and cis-acting elements analysis revealed that MACPFs play a role in resistance to abiotic stresses, and some selected GhMACPFs were able to respond to the PEG and cold stresses. Co-expression analysis showed that GhMACPFs might interact with valine-glutamine (VQ), WRKY, and Apetala 2 (AP2)/ethylene responsive factor (ERF) domain-containing genes under cold stress. In addition, silencing endogenous GhMACPF26 in cotton by the virus-induced gene silencing (VIGS) method indicated that GhMACPF26 negatively regulates cold tolerance. Our data provided a comprehensive phylogenetic evolutionary view of Gossypium MACPFs. The MACPFs may work together with multiple transcriptional factors and play roles in acclimation to abiotic stress, especially cold stress in cotton.https://www.frontiersin.org/articles/10.3389/fpls.2021.684227/fullGossypiummembrane attack complex/perforin (MACPF)cold stressVIGSWGCNA |
| spellingShingle | Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress Frontiers in Plant Science Gossypium membrane attack complex/perforin (MACPF) cold stress VIGS WGCNA |
| title | Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress |
| title_full | Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress |
| title_fullStr | Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress |
| title_full_unstemmed | Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress |
| title_short | Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress |
| title_sort | phylogenetic analysis of the membrane attack complex perforin domain containing proteins in gossypium and the role of ghmacpf26 in cotton under cold stress |
| topic | Gossypium membrane attack complex/perforin (MACPF) cold stress VIGS WGCNA |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.684227/full |
| work_keys_str_mv | AT pengyunchen phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT hongliangjian phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT feiwei phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT lijiaogu phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT tinglihu phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT xiaoyanlv phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT xiaohaoguo phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT jianhualu phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT liangma phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT hantaowang phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT aiminwu phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT guangzhimao phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT shuxunyu phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress AT henglingwei phylogeneticanalysisofthemembraneattackcomplexperforindomaincontainingproteinsingossypiumandtheroleofghmacpf26incottonundercoldstress |