AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i>
Abscisic acid (ABA, <b>1</b>) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new met...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/1/161 |
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| author | Tomoaki Anabuki Keisuke Ohashi Taichi E. Takasuka Hideyuki Matsuura Kosaku Takahashi |
| author_facet | Tomoaki Anabuki Keisuke Ohashi Taichi E. Takasuka Hideyuki Matsuura Kosaku Takahashi |
| author_sort | Tomoaki Anabuki |
| collection | DOAJ |
| description | Abscisic acid (ABA, <b>1</b>) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of <i>Arabidopsis thaliana</i>. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds. |
| first_indexed | 2024-03-10T03:30:31Z |
| format | Article |
| id | doaj.art-0a916e133fd24c6b8a78d56ef6fd8abe |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:30:31Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0a916e133fd24c6b8a78d56ef6fd8abe2023-11-23T11:57:37ZengMDPI AGMolecules1420-30492021-12-0127116110.3390/molecules27010161AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i>Tomoaki Anabuki0Keisuke Ohashi1Taichi E. Takasuka2Hideyuki Matsuura3Kosaku Takahashi4Division of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, JapanGraduate School of Global Food Resources, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-0809, JapanDivision of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, JapanDivision of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, JapanDepartment of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 165-8502, JapanAbscisic acid (ABA, <b>1</b>) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of <i>Arabidopsis thaliana</i>. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds.https://www.mdpi.com/1420-3049/27/1/161abscisic acidaffinity chromatography<i>Arabidopsis thaliana</i>click chemistrytarget proteinthioredoxin |
| spellingShingle | Tomoaki Anabuki Keisuke Ohashi Taichi E. Takasuka Hideyuki Matsuura Kosaku Takahashi AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> Molecules abscisic acid affinity chromatography <i>Arabidopsis thaliana</i> click chemistry target protein thioredoxin |
| title | AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> |
| title_full | AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> |
| title_fullStr | AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> |
| title_short | AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i> |
| title_sort | attrxh3 a thioredoxin is identified as an abscisic acidbinding protein in i arabidopsis thaliana i |
| topic | abscisic acid affinity chromatography <i>Arabidopsis thaliana</i> click chemistry target protein thioredoxin |
| url | https://www.mdpi.com/1420-3049/27/1/161 |
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