<it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response
<p>Abstract</p> <p>Background</p> <p>Proteins that are involved in regulation of cell division and cell cycle progression remain undefined in <it>Mycobacterium tuberculosis</it>. In addition, there is a growing appreciation that regulation of cell replicatio...
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| Materiálatiipa: | Artihkal |
| Giella: | English |
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BMC
2011-04-01
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| Ráidu: | BMC Microbiology |
| Fáttát: | |
| Liŋkkat: | http://www.biomedcentral.com/1471-2180/11/79 |
| _version_ | 1828493374531829760 |
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| author | Crew Rebecca England Kathleen Slayden Richard A |
| author_facet | Crew Rebecca England Kathleen Slayden Richard A |
| author_sort | Crew Rebecca |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Proteins that are involved in regulation of cell division and cell cycle progression remain undefined in <it>Mycobacterium tuberculosis</it>. In addition, there is a growing appreciation that regulation of cell replication at the point of division is important in establishing a non-replicating persistent state. Accordingly, the objective of this study was to use a systematic approach consisting of consensus-modeling bioinformatics, ultrastructural analysis, and transcriptional mapping to identify septum regulatory proteins that participate in adaptive metabolic responses in <it>M. tuberculosis</it>.</p> <p>Results</p> <p>Septum site determining protein (Ssd), encoded by <it>rv3660c </it>was discovered to be an ortholog of septum site regulating proteins in actinobacteria by bioinformatics analysis. Increased expression of <it>ssd </it>in <it>M. smegmatis </it>and <it>M. tuberculosis </it>inhibited septum formation resulting in elongated cells devoid of septa. Transcriptional mapping in <it>M. tuberculosis </it>showed that increased <it>ssd </it>expression elicited a unique response including the dormancy regulon and alternative sigma factors that are thought to play a role in adaptive metabolism. Disruption of <it>rv3660c </it>by transposon insertion negated the unique transcriptional response and led to a reduced bacterial length.</p> <p>Conclusions</p> <p>This study establishes the first connection between a septum regulatory protein and induction of alternative metabolism consisting of alternative sigma factors and the dormancy regulon that is associated with establishing a non-replicating persistent intracellular lifestyle. The identification of a regulatory component involved in cell cycle regulation linked to the dormancy response, whether directly or indirectly, provides a foundation for additional studies and furthers our understanding of the complex mechanisms involved in establishing a non-replicating state and resumption of growth.</p> |
| first_indexed | 2024-12-11T11:36:49Z |
| format | Article |
| id | doaj.art-0a934194bb354d2a8ee5bf2d287967c0 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T11:36:49Z |
| publishDate | 2011-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-0a934194bb354d2a8ee5bf2d287967c02022-12-22T01:08:43ZengBMCBMC Microbiology1471-21802011-04-011117910.1186/1471-2180-11-79<it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress responseCrew RebeccaEngland KathleenSlayden Richard A<p>Abstract</p> <p>Background</p> <p>Proteins that are involved in regulation of cell division and cell cycle progression remain undefined in <it>Mycobacterium tuberculosis</it>. In addition, there is a growing appreciation that regulation of cell replication at the point of division is important in establishing a non-replicating persistent state. Accordingly, the objective of this study was to use a systematic approach consisting of consensus-modeling bioinformatics, ultrastructural analysis, and transcriptional mapping to identify septum regulatory proteins that participate in adaptive metabolic responses in <it>M. tuberculosis</it>.</p> <p>Results</p> <p>Septum site determining protein (Ssd), encoded by <it>rv3660c </it>was discovered to be an ortholog of septum site regulating proteins in actinobacteria by bioinformatics analysis. Increased expression of <it>ssd </it>in <it>M. smegmatis </it>and <it>M. tuberculosis </it>inhibited septum formation resulting in elongated cells devoid of septa. Transcriptional mapping in <it>M. tuberculosis </it>showed that increased <it>ssd </it>expression elicited a unique response including the dormancy regulon and alternative sigma factors that are thought to play a role in adaptive metabolism. Disruption of <it>rv3660c </it>by transposon insertion negated the unique transcriptional response and led to a reduced bacterial length.</p> <p>Conclusions</p> <p>This study establishes the first connection between a septum regulatory protein and induction of alternative metabolism consisting of alternative sigma factors and the dormancy regulon that is associated with establishing a non-replicating persistent intracellular lifestyle. The identification of a regulatory component involved in cell cycle regulation linked to the dormancy response, whether directly or indirectly, provides a foundation for additional studies and furthers our understanding of the complex mechanisms involved in establishing a non-replicating state and resumption of growth.</p>http://www.biomedcentral.com/1471-2180/11/79<it>Mycobacterium tuberculosis</it>dormancyDos regulonseptum site determining proteincell division |
| spellingShingle | Crew Rebecca England Kathleen Slayden Richard A <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response BMC Microbiology <it>Mycobacterium tuberculosis</it> dormancy Dos regulon septum site determining protein cell division |
| title | <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| title_full | <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| title_fullStr | <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| title_full_unstemmed | <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| title_short | <it>Mycobacterium tuberculosis </it>septum site determining protein, Ssd encoded by <it>rv3660c</it>, promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| title_sort | it mycobacterium tuberculosis it septum site determining protein ssd encoded by it rv3660c it promotes filamentation and elicits an alternative metabolic and dormancy stress response |
| topic | <it>Mycobacterium tuberculosis</it> dormancy Dos regulon septum site determining protein cell division |
| url | http://www.biomedcentral.com/1471-2180/11/79 |
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