miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK
Abstract Background microRNAs (miRNAs) have been implicated to play crucial roles in carcinogenesis. miR‐1249‐3p was reported to be abnormally expressed in multiple human cancers. However, its biological role and the associated underlying mechanisms in hepatocellular carcinoma (HCC) remain largely u...
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | Molecular Genetics & Genomic Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mgg3.867 |
| _version_ | 1797301101899284480 |
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| author | Hongchun Shu Jia Hu Huiqiu Deng |
| author_facet | Hongchun Shu Jia Hu Huiqiu Deng |
| author_sort | Hongchun Shu |
| collection | DOAJ |
| description | Abstract Background microRNAs (miRNAs) have been implicated to play crucial roles in carcinogenesis. miR‐1249‐3p was reported to be abnormally expressed in multiple human cancers. However, its biological role and the associated underlying mechanisms in hepatocellular carcinoma (HCC) remain largely unknown. Methods miR‐1249‐3p expression level in HCC cell lines and normal cell line was measured by quantitative real‐time PCR. Role of miR‐1249‐3p on HCC cell proliferation, colony formation, and invasion was examined by cell counting kit‐8 assay, colony formation assay, and transwell invasion assay, respectively. Luciferase activity reporter assay and western blot were performed to validate whether heterogeneous nuclear ribonucleoprotein K (HNRNPK) was a direct target of miR‐1249‐3p. Effect of miR‐1249‐3p on overall survival of HCC patients was analyzed at KM Plotter website. Results We found miR‐1249‐3p expression level was increased, while HNRNPK expression level was decreased in HCC cell lines compared with normal cell line. Knockdown miR‐1249‐3p expression inhibits HCC cell proliferation, colony formation, and cell invasion through regulating HNRNPK in vitro. We also showed high miR‐1249‐3p expression was a predictor for poor overall survival of HCC patients. Conclusions These findings about miR‐1249‐3p/HNRNPK pair provide a novel therapeutic method for HCC patients. |
| first_indexed | 2024-03-07T23:16:34Z |
| format | Article |
| id | doaj.art-0a9825cdcfd546eab8e5aadf4192ffa4 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-03-07T23:16:34Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-0a9825cdcfd546eab8e5aadf4192ffa42024-02-21T10:29:27ZengWileyMolecular Genetics & Genomic Medicine2324-92692019-10-01710n/an/a10.1002/mgg3.867miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPKHongchun Shu0Jia Hu1Huiqiu Deng2Department of Gastroenterology, Jiangxi Institute of Gastroenterology & Hepatology The First Affiliated Hospital of Nanchang University Nanchang P. R. ChinaDepartment of Gastroenterology, Jiangxi Institute of Gastroenterology & Hepatology The First Affiliated Hospital of Nanchang University Nanchang P. R. ChinaGastroenterology Department ShangRao People’s Hospital Shangrao P. R. ChinaAbstract Background microRNAs (miRNAs) have been implicated to play crucial roles in carcinogenesis. miR‐1249‐3p was reported to be abnormally expressed in multiple human cancers. However, its biological role and the associated underlying mechanisms in hepatocellular carcinoma (HCC) remain largely unknown. Methods miR‐1249‐3p expression level in HCC cell lines and normal cell line was measured by quantitative real‐time PCR. Role of miR‐1249‐3p on HCC cell proliferation, colony formation, and invasion was examined by cell counting kit‐8 assay, colony formation assay, and transwell invasion assay, respectively. Luciferase activity reporter assay and western blot were performed to validate whether heterogeneous nuclear ribonucleoprotein K (HNRNPK) was a direct target of miR‐1249‐3p. Effect of miR‐1249‐3p on overall survival of HCC patients was analyzed at KM Plotter website. Results We found miR‐1249‐3p expression level was increased, while HNRNPK expression level was decreased in HCC cell lines compared with normal cell line. Knockdown miR‐1249‐3p expression inhibits HCC cell proliferation, colony formation, and cell invasion through regulating HNRNPK in vitro. We also showed high miR‐1249‐3p expression was a predictor for poor overall survival of HCC patients. Conclusions These findings about miR‐1249‐3p/HNRNPK pair provide a novel therapeutic method for HCC patients.https://doi.org/10.1002/mgg3.867cell eventshepatocellular carcinomaHNRNPKmiR‐1249‐3p |
| spellingShingle | Hongchun Shu Jia Hu Huiqiu Deng miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK Molecular Genetics & Genomic Medicine cell events hepatocellular carcinoma HNRNPK miR‐1249‐3p |
| title | miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK |
| title_full | miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK |
| title_fullStr | miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK |
| title_full_unstemmed | miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK |
| title_short | miR‐1249‐3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK |
| title_sort | mir 1249 3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting hnrnpk |
| topic | cell events hepatocellular carcinoma HNRNPK miR‐1249‐3p |
| url | https://doi.org/10.1002/mgg3.867 |
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