Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center
Introduction. The relevance of studying the circulation of human respiratory viruses among laboratory primates is associated with the need to test vaccines and antiviral drugs against these infections on monkeys. The aim of this work was to study the prevalence of serological and molecular markers...
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Central Research Institute for Epidemiology
2022-01-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/576/354 |
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| author | L. I. Korzaya D. I. Dogadov A. M. Goncharenko A. A. Karlsen K. K. Kyuregyan M. I. Mikhailov |
| author_facet | L. I. Korzaya D. I. Dogadov A. M. Goncharenko A. A. Karlsen K. K. Kyuregyan M. I. Mikhailov |
| author_sort | L. I. Korzaya |
| collection | DOAJ |
| description | Introduction. The relevance of studying the circulation of human respiratory viruses among laboratory primates is associated with the need to test vaccines and antiviral drugs against these infections on monkeys.
The aim of this work was to study the prevalence of serological and molecular markers of human respiratory viral infections in laboratory primates born at the Adler Primate Center and in imported monkeys.
Material and methods. Blood serum samples (n = 1971) and lung autopsy material (n = 26) were obtained from different monkey species. These samples were tested for the presence of serological markers of measles, parainfluenza (PI) types 1, 2, 3, influenza A and B, respiratory syncytial (RS) and adenovirus infections using enzyme immunoassay (ELISA). Detection of RS virus, metapneumovirus, PI virus types 1–4, rhinovirus, coronavirus, and adenoviruses B, C, E and bocavirus nucleic acids in this material was performed by reverse transcription polymerase chain reaction (RT-PCR).
Results and discussion. The overall prevalence of antibodies (Abs) among all monkeys was low and amounted 11.3% (95% CI: 9.2–13.7%, n = 811) for measles virus, 8.9% (95% CI: 6.2–12.2%, n = 381) for PI type 3 virus, 2.5% (95% CI: 0.8–5.6%, n = 204) for PI type 1 virus, and 7.7% (95% CI: 3.8–13.7%, n = 130) for adenoviruses. When testing 26 autopsy lung samples from monkeys of different species that died from pneumonia, 2 samples from Anubis baboons (Papio аnubis) were positive for of parainfluenza virus type 3 RNA.
Conclusion. Our data suggest the importance of the strict adherence to the terms of quarantine and mandatory testing of monkey sera for the presence of IgM antibodies to the measles virus that indicate the recent infection. The role of PI virus type 3 in the pathology of the respiratory tract in Anubis baboons has been established. |
| first_indexed | 2024-03-13T00:08:21Z |
| format | Article |
| id | doaj.art-0a991515c8df441aae478f8382e270a9 |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:21Z |
| publishDate | 2022-01-01 |
| publisher | Central Research Institute for Epidemiology |
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| series | Вопросы вирусологии |
| spelling | doaj.art-0a991515c8df441aae478f8382e270a92023-07-12T19:55:59ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972022-01-0166642543310.36233/0507-4088-77413Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate centerL. I. Korzaya0https://orcid.org/0000-0003-2259-5773D. I. Dogadov1https://orcid.org/0000-0003-1596-0509A. M. Goncharenko2https://orcid.org/0000-0002-6979-9784A. A. Karlsen3https://orcid.org/0000-0002-6013-7768K. K. Kyuregyan4https://orcid.org/0000-0002-3599-117XM. I. Mikhailov5https://orcid.org/0000-0002-6636-6801FSBRI «Research Institute of Medical Primatology» of the Ministry of Higher Education and Science of RussiaFSBRI «Research Institute of Medical Primatology» of the Ministry of Higher Education and Science of RussiaFSBRI «Research Institute of Medical Primatology» of the Ministry of Higher Education and Science of RussiaFSBEI FPE «Russian Medical Academy of Continuous Professional Education» of the Ministry of Health of RussiaFSBEI FPE «Russian Medical Academy of Continuous Professional Education» of the Ministry of Health of RussiaFSBEI FPE «Russian Medical Academy of Continuous Professional Education» of the Ministry of Health of RussiaIntroduction. The relevance of studying the circulation of human respiratory viruses among laboratory primates is associated with the need to test vaccines and antiviral drugs against these infections on monkeys. The aim of this work was to study the prevalence of serological and molecular markers of human respiratory viral infections in laboratory primates born at the Adler Primate Center and in imported monkeys. Material and methods. Blood serum samples (n = 1971) and lung autopsy material (n = 26) were obtained from different monkey species. These samples were tested for the presence of serological markers of measles, parainfluenza (PI) types 1, 2, 3, influenza A and B, respiratory syncytial (RS) and adenovirus infections using enzyme immunoassay (ELISA). Detection of RS virus, metapneumovirus, PI virus types 1–4, rhinovirus, coronavirus, and adenoviruses B, C, E and bocavirus nucleic acids in this material was performed by reverse transcription polymerase chain reaction (RT-PCR). Results and discussion. The overall prevalence of antibodies (Abs) among all monkeys was low and amounted 11.3% (95% CI: 9.2–13.7%, n = 811) for measles virus, 8.9% (95% CI: 6.2–12.2%, n = 381) for PI type 3 virus, 2.5% (95% CI: 0.8–5.6%, n = 204) for PI type 1 virus, and 7.7% (95% CI: 3.8–13.7%, n = 130) for adenoviruses. When testing 26 autopsy lung samples from monkeys of different species that died from pneumonia, 2 samples from Anubis baboons (Papio аnubis) were positive for of parainfluenza virus type 3 RNA. Conclusion. Our data suggest the importance of the strict adherence to the terms of quarantine and mandatory testing of monkey sera for the presence of IgM antibodies to the measles virus that indicate the recent infection. The role of PI virus type 3 in the pathology of the respiratory tract in Anubis baboons has been established.https://virusjour.crie.ru/jour/article/viewFile/576/354human respiratory viruses – influenza a, influenza b, parainfluenza types 1, 2, 3, 4, respiratory syncytial (rs) virus, adenovirusmeasles virusantibodies (igg, igm)enzyme-linked immunosorbent assay (elisa)monkeys |
| spellingShingle | L. I. Korzaya D. I. Dogadov A. M. Goncharenko A. A. Karlsen K. K. Kyuregyan M. I. Mikhailov Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center Вопросы вирусологии human respiratory viruses – influenza a, influenza b, parainfluenza types 1, 2, 3, 4, respiratory syncytial (rs) virus, adenovirus measles virus antibodies (igg, igm) enzyme-linked immunosorbent assay (elisa) monkeys |
| title | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
| title_full | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
| title_fullStr | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
| title_full_unstemmed | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
| title_short | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
| title_sort | prevalence of laboratory markers of human respiratory viruses in monkeys of adler primate center |
| topic | human respiratory viruses – influenza a, influenza b, parainfluenza types 1, 2, 3, 4, respiratory syncytial (rs) virus, adenovirus measles virus antibodies (igg, igm) enzyme-linked immunosorbent assay (elisa) monkeys |
| url | https://virusjour.crie.ru/jour/article/viewFile/576/354 |
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