Analysis of the impact of cryopreservation and theophylline on motility of sperm
Objective: Sperm parameters, particularly motility, decrease during cryopreservation. Theophylline generally enhances sperm motility. We analyzed effects of theophylline and freezing on sperm motility.Design: Experimental study.Setting: Private IVF lab.Setting: IVF lab of Mehrgan Hospital. Method: 2...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-06-01
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| Series: | Middle East Fertility Society Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569017301115 |
| _version_ | 1818808546925477888 |
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| author | Elaheh Gorji Mir Mehrdad Farsi Soraya Khafri Hamid Shafi |
| author_facet | Elaheh Gorji Mir Mehrdad Farsi Soraya Khafri Hamid Shafi |
| author_sort | Elaheh Gorji |
| collection | DOAJ |
| description | Objective: Sperm parameters, particularly motility, decrease during cryopreservation. Theophylline generally enhances sperm motility. We analyzed effects of theophylline and freezing on sperm motility.Design: Experimental study.Setting: Private IVF lab.Setting: IVF lab of Mehrgan Hospital. Method: 22–55 year-old men participated in this study (30 fresh ejaculation and 8 TESE samples). After sperm analysis, we added theophylline (40 mM) to half of our samples as case group to compare motility with the remaining samples as control group. Cryopreservation was performed in two groups. After thawing, motility of both groups was recorded. Furthermore, theophylline (40 mM) was applied to both groups after thawing again. Result: After adding theophylline, sperm motility improved significantly in all samples. Sperm motility reduced in control group more than the study group after freeze-thaw procedure (P < 0.002, normal morphology <5%). Sperm motility was not enhanced significantly by re-adding of theophylline to the two groups. Interactions between stages and groups were statistically significant in semen and biopsy samples (p < 0.001). Conclusion: Adding theophylline before freezing can preserve motility of sperms in samples with different parameters and even sperms extracted in testicular biopsy. Theophylline may have protective impact on sperms in freezing procedure. Keywords: Sperm motility, Theophylline, Freezing, Morphology, Biopsy |
| first_indexed | 2024-12-18T19:43:17Z |
| format | Article |
| id | doaj.art-0a9b6636ac8541769b0a1a548634d78c |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-18T19:43:17Z |
| publishDate | 2018-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-0a9b6636ac8541769b0a1a548634d78c2022-12-21T20:55:21ZengSpringerOpenMiddle East Fertility Society Journal1110-56902018-06-0123298102Analysis of the impact of cryopreservation and theophylline on motility of spermElaheh Gorji0Mir Mehrdad Farsi1Soraya Khafri2Hamid Shafi3Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, IranDepartment of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran; Corresponding author.Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, IranDepartment of Urology, School of Medicine, Babol University of Medical Sciences, Babol, IranObjective: Sperm parameters, particularly motility, decrease during cryopreservation. Theophylline generally enhances sperm motility. We analyzed effects of theophylline and freezing on sperm motility.Design: Experimental study.Setting: Private IVF lab.Setting: IVF lab of Mehrgan Hospital. Method: 22–55 year-old men participated in this study (30 fresh ejaculation and 8 TESE samples). After sperm analysis, we added theophylline (40 mM) to half of our samples as case group to compare motility with the remaining samples as control group. Cryopreservation was performed in two groups. After thawing, motility of both groups was recorded. Furthermore, theophylline (40 mM) was applied to both groups after thawing again. Result: After adding theophylline, sperm motility improved significantly in all samples. Sperm motility reduced in control group more than the study group after freeze-thaw procedure (P < 0.002, normal morphology <5%). Sperm motility was not enhanced significantly by re-adding of theophylline to the two groups. Interactions between stages and groups were statistically significant in semen and biopsy samples (p < 0.001). Conclusion: Adding theophylline before freezing can preserve motility of sperms in samples with different parameters and even sperms extracted in testicular biopsy. Theophylline may have protective impact on sperms in freezing procedure. Keywords: Sperm motility, Theophylline, Freezing, Morphology, Biopsyhttp://www.sciencedirect.com/science/article/pii/S1110569017301115 |
| spellingShingle | Elaheh Gorji Mir Mehrdad Farsi Soraya Khafri Hamid Shafi Analysis of the impact of cryopreservation and theophylline on motility of sperm Middle East Fertility Society Journal |
| title | Analysis of the impact of cryopreservation and theophylline on motility of sperm |
| title_full | Analysis of the impact of cryopreservation and theophylline on motility of sperm |
| title_fullStr | Analysis of the impact of cryopreservation and theophylline on motility of sperm |
| title_full_unstemmed | Analysis of the impact of cryopreservation and theophylline on motility of sperm |
| title_short | Analysis of the impact of cryopreservation and theophylline on motility of sperm |
| title_sort | analysis of the impact of cryopreservation and theophylline on motility of sperm |
| url | http://www.sciencedirect.com/science/article/pii/S1110569017301115 |
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