Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR
The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a v...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2014-12-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262299 |
| _version_ | 1831770752393674752 |
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| author | Seung-Yeol Lee Mi-Chi Yea Chang-Gi Back Kwang-Shik Choi In-Kyu Kang Su-Heon Lee Hee-Young Jung |
| author_facet | Seung-Yeol Lee Mi-Chi Yea Chang-Gi Back Kwang-Shik Choi In-Kyu Kang Su-Heon Lee Hee-Young Jung |
| author_sort | Seung-Yeol Lee |
| collection | DOAJ |
| description | The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield. |
| first_indexed | 2024-12-22T07:46:54Z |
| format | Article |
| id | doaj.art-0a9fd8de25cd43929214d7e4019558f1 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-22T07:46:54Z |
| publishDate | 2014-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-0a9fd8de25cd43929214d7e4019558f12022-12-21T18:33:37ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542014-12-0130444544910.5423/PPJ.NT.05.2014.0041PPJ.NT.05.2014.0041Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCRSeung-Yeol Lee0Mi-Chi Yea1Chang-Gi Back2Kwang-Shik Choi3In-Kyu Kang4Su-Heon Lee5Hee-Young Jung6College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Natural Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaCollege of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, KoreaThe incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262299CNRMVCGRMVDuplex RT-PCRInfection rate |
| spellingShingle | Seung-Yeol Lee Mi-Chi Yea Chang-Gi Back Kwang-Shik Choi In-Kyu Kang Su-Heon Lee Hee-Young Jung Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR The Plant Pathology Journal CNRMV CGRMV Duplex RT-PCR Infection rate |
| title | Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR |
| title_full | Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR |
| title_fullStr | Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR |
| title_full_unstemmed | Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR |
| title_short | Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR |
| title_sort | survey of cherry necrotic rusty mottle virus and cherry green ring mottle virus incidence in korea by duplex rt pcr |
| topic | CNRMV CGRMV Duplex RT-PCR Infection rate |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262299 |
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