Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice
The leishmaniases are a group of diseases caused by Leishmania parasites, which have different clinical manifestations. Leishmania (Leishmania) amazonensis is endemic in South America and causes cutaneous leishmaniasis (CL), which can evolve into a diffuse form, characterized by an anergic immune re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00088/full |
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| author | Izabella Pereira da Silva Bezerra Gabriel Oliveira-Silva Danielle Sophia Ferreira Santos Braga Mirian França de Mello Juliana Elena Silveira Pratti Joyce Carvalho Pereira Alessandra Marcia da Fonseca-Martins Luan Firmino-Cruz Diogo Maciel-Oliveira Tadeu Diniz Ramos André Macedo Vale Daniel Claudio Oliveira Gomes Bartira Rossi-Bergmann Herbert Leonel de Matos Guedes Herbert Leonel de Matos Guedes |
| author_facet | Izabella Pereira da Silva Bezerra Gabriel Oliveira-Silva Danielle Sophia Ferreira Santos Braga Mirian França de Mello Juliana Elena Silveira Pratti Joyce Carvalho Pereira Alessandra Marcia da Fonseca-Martins Luan Firmino-Cruz Diogo Maciel-Oliveira Tadeu Diniz Ramos André Macedo Vale Daniel Claudio Oliveira Gomes Bartira Rossi-Bergmann Herbert Leonel de Matos Guedes Herbert Leonel de Matos Guedes |
| author_sort | Izabella Pereira da Silva Bezerra |
| collection | DOAJ |
| description | The leishmaniases are a group of diseases caused by Leishmania parasites, which have different clinical manifestations. Leishmania (Leishmania) amazonensis is endemic in South America and causes cutaneous leishmaniasis (CL), which can evolve into a diffuse form, characterized by an anergic immune response. Since the leishmaniases mainly affect poor populations, it is important to understand the involvement of immunonutrition, how the immune system is modulated by dietary nutrients and the effect this has on Leishmania infection. Vitamin D3 (VitD) is an immunonutrient obtained from diet or endogenously synthesized, which suppresses Th1 and Th17 responses by favoring T helper (Th) 2 and regulatory T cell (Treg) generation. Based on these findings, this study aims to evaluate dietary VitD influence on L. (L.) amazonensis experimental infection in C57BL/6 and BALB/c mice. Thus, C57BL/6 and BALB/c VitD deficient (VDD) mice were generated through dietary VitD restriction 45 days prior to infection. Both strains of VDD mice showed a more controlled lesion development compared to mice on a regular diet (Ctrl). There were no differences in serum levels of anti-Leishmania IgG1 and IgG2a, but there was a decrease in IgE levels in BALB/c VDD mice. Although CD4+ T cell number was not changed, the CD4+ IFN-y+ T cell population was increased in both absolute number and percentage in C57BL/6 and BALB/c VDD mice compared to Ctrl mice. There was also no difference in IL-4 and IL-17 production, however, there was reduction of IL-10 production in VDD mice. Together, our data indicate that VitD contributes to murine cutaneous leishmaniasis susceptibility and that the Th1 cell population may be related to the resistance of VDD mice to L. (L.) amazonensis infection. |
| first_indexed | 2024-12-22T03:37:02Z |
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| id | doaj.art-0aa2763a6a3d45b4970b93706123982a |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-22T03:37:02Z |
| publishDate | 2019-04-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0aa2763a6a3d45b4970b93706123982a2022-12-21T18:40:21ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-04-01910.3389/fcimb.2019.00088448329Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in MiceIzabella Pereira da Silva Bezerra0Gabriel Oliveira-Silva1Danielle Sophia Ferreira Santos Braga2Mirian França de Mello3Juliana Elena Silveira Pratti4Joyce Carvalho Pereira5Alessandra Marcia da Fonseca-Martins6Luan Firmino-Cruz7Diogo Maciel-Oliveira8Tadeu Diniz Ramos9André Macedo Vale10Daniel Claudio Oliveira Gomes11Bartira Rossi-Bergmann12Herbert Leonel de Matos Guedes13Herbert Leonel de Matos Guedes14Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Doenças Infecciosas/Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo Multidisciplinar de Pesquisa UFRJ – Xerém em Biologia, UFRJ Campus Duque de Caxias Professor Geraldo Cidade – Universidade Federal do Rio de Janeiro, Duque de Caxias, BrazilThe leishmaniases are a group of diseases caused by Leishmania parasites, which have different clinical manifestations. Leishmania (Leishmania) amazonensis is endemic in South America and causes cutaneous leishmaniasis (CL), which can evolve into a diffuse form, characterized by an anergic immune response. Since the leishmaniases mainly affect poor populations, it is important to understand the involvement of immunonutrition, how the immune system is modulated by dietary nutrients and the effect this has on Leishmania infection. Vitamin D3 (VitD) is an immunonutrient obtained from diet or endogenously synthesized, which suppresses Th1 and Th17 responses by favoring T helper (Th) 2 and regulatory T cell (Treg) generation. Based on these findings, this study aims to evaluate dietary VitD influence on L. (L.) amazonensis experimental infection in C57BL/6 and BALB/c mice. Thus, C57BL/6 and BALB/c VitD deficient (VDD) mice were generated through dietary VitD restriction 45 days prior to infection. Both strains of VDD mice showed a more controlled lesion development compared to mice on a regular diet (Ctrl). There were no differences in serum levels of anti-Leishmania IgG1 and IgG2a, but there was a decrease in IgE levels in BALB/c VDD mice. Although CD4+ T cell number was not changed, the CD4+ IFN-y+ T cell population was increased in both absolute number and percentage in C57BL/6 and BALB/c VDD mice compared to Ctrl mice. There was also no difference in IL-4 and IL-17 production, however, there was reduction of IL-10 production in VDD mice. Together, our data indicate that VitD contributes to murine cutaneous leishmaniasis susceptibility and that the Th1 cell population may be related to the resistance of VDD mice to L. (L.) amazonensis infection.https://www.frontiersin.org/article/10.3389/fcimb.2019.00088/fullleishmaniasisLeishmania amazonensisvitamin DTh1immunonutritionIL-10 |
| spellingShingle | Izabella Pereira da Silva Bezerra Gabriel Oliveira-Silva Danielle Sophia Ferreira Santos Braga Mirian França de Mello Juliana Elena Silveira Pratti Joyce Carvalho Pereira Alessandra Marcia da Fonseca-Martins Luan Firmino-Cruz Diogo Maciel-Oliveira Tadeu Diniz Ramos André Macedo Vale Daniel Claudio Oliveira Gomes Bartira Rossi-Bergmann Herbert Leonel de Matos Guedes Herbert Leonel de Matos Guedes Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice Frontiers in Cellular and Infection Microbiology leishmaniasis Leishmania amazonensis vitamin D Th1 immunonutrition IL-10 |
| title | Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice |
| title_full | Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice |
| title_fullStr | Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice |
| title_full_unstemmed | Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice |
| title_short | Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice |
| title_sort | dietary vitamin d3 deficiency increases resistance to leishmania leishmania amazonensis infection in mice |
| topic | leishmaniasis Leishmania amazonensis vitamin D Th1 immunonutrition IL-10 |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00088/full |
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