Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>)
Phenolic compounds can act as a substrate for colonic resident microbiota. Once the metabolites are absorbed and distributed throughout the body, they can have diverse effects on the gut. The objective of this study was to evaluate the effects of the intra-amniotic administration of a chia phenolic...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/16/3643 |
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| author | Marcella Duarte Villas Mishima Hércia Stampini Duarte Martino Nikolai Kolba Nikita Agarwal Cydney Jackson Bárbara Pereira da Silva Mariana Grancieri Andressa de Assis Vinícius Parzanini Brilhante de São José Elad Tako |
| author_facet | Marcella Duarte Villas Mishima Hércia Stampini Duarte Martino Nikolai Kolba Nikita Agarwal Cydney Jackson Bárbara Pereira da Silva Mariana Grancieri Andressa de Assis Vinícius Parzanini Brilhante de São José Elad Tako |
| author_sort | Marcella Duarte Villas Mishima |
| collection | DOAJ |
| description | Phenolic compounds can act as a substrate for colonic resident microbiota. Once the metabolites are absorbed and distributed throughout the body, they can have diverse effects on the gut. The objective of this study was to evaluate the effects of the intra-amniotic administration of a chia phenolic extract on intestinal inflammation, intestinal barrier, brush border membrane functionality, intestinal microbiota, and morphology in vivo (<i>Gallus gallus</i> model). Cornish-cross fertile broiler eggs, at 17 days of embryonic incubation, were separated into groups as follows: non-injected (NI; this group did not receive an injection); 18 MΩ H<sub>2</sub>O (H<sub>2</sub>O; injected with ultrapure water), and 10 mg/mL (1%) chia phenolic extract (CPE; injected with phenolic extract diluted in ultrapure water). Immediately after hatch (21 days), chickens were euthanized and their small intestine, cecum, and cecum content were collected and analyzed. The chia phenolic extract reduced the tumor necrosis factor-alpha (TNF-α) and increased the sucrose isomaltase (SI) gene expression, reduced the <i>Bifidobacterium</i> and <i>E. coli</i> populations, reduced the Paneth cell diameter, increased depth crypt, and maintained villus height compared to the non-injected control group. Chia phenolic extract may be a promising beneficial compound for improving intestinal health, demonstrating positive changes in intestinal inflammation, functionality, microbiota, and morphology. |
| first_indexed | 2024-03-10T23:40:12Z |
| format | Article |
| id | doaj.art-0aa621378fe844ae9002e67537a82e8e |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T23:40:12Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-0aa621378fe844ae9002e67537a82e8e2023-11-19T02:31:08ZengMDPI AGNutrients2072-66432023-08-011516364310.3390/nu15163643Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>)Marcella Duarte Villas Mishima0Hércia Stampini Duarte Martino1Nikolai Kolba2Nikita Agarwal3Cydney Jackson4Bárbara Pereira da Silva5Mariana Grancieri6Andressa de Assis7Vinícius Parzanini Brilhante de São José8Elad Tako9Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USADepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USADepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USADepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USADepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570-900, MG, BrazilDepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USAPhenolic compounds can act as a substrate for colonic resident microbiota. Once the metabolites are absorbed and distributed throughout the body, they can have diverse effects on the gut. The objective of this study was to evaluate the effects of the intra-amniotic administration of a chia phenolic extract on intestinal inflammation, intestinal barrier, brush border membrane functionality, intestinal microbiota, and morphology in vivo (<i>Gallus gallus</i> model). Cornish-cross fertile broiler eggs, at 17 days of embryonic incubation, were separated into groups as follows: non-injected (NI; this group did not receive an injection); 18 MΩ H<sub>2</sub>O (H<sub>2</sub>O; injected with ultrapure water), and 10 mg/mL (1%) chia phenolic extract (CPE; injected with phenolic extract diluted in ultrapure water). Immediately after hatch (21 days), chickens were euthanized and their small intestine, cecum, and cecum content were collected and analyzed. The chia phenolic extract reduced the tumor necrosis factor-alpha (TNF-α) and increased the sucrose isomaltase (SI) gene expression, reduced the <i>Bifidobacterium</i> and <i>E. coli</i> populations, reduced the Paneth cell diameter, increased depth crypt, and maintained villus height compared to the non-injected control group. Chia phenolic extract may be a promising beneficial compound for improving intestinal health, demonstrating positive changes in intestinal inflammation, functionality, microbiota, and morphology.https://www.mdpi.com/2072-6643/15/16/3643<i>Salvia hispanica</i> L.phenolic compoundsintra-amniotic administrationintestinal healthBBM |
| spellingShingle | Marcella Duarte Villas Mishima Hércia Stampini Duarte Martino Nikolai Kolba Nikita Agarwal Cydney Jackson Bárbara Pereira da Silva Mariana Grancieri Andressa de Assis Vinícius Parzanini Brilhante de São José Elad Tako Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) Nutrients <i>Salvia hispanica</i> L. phenolic compounds intra-amniotic administration intestinal health BBM |
| title | Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) |
| title_full | Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) |
| title_fullStr | Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) |
| title_full_unstemmed | Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) |
| title_short | Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>) |
| title_sort | chia phenolic extract appear to improve small intestinal functionality morphology bacterial populations and inflammation biomarkers in vivo i gallus gallus i |
| topic | <i>Salvia hispanica</i> L. phenolic compounds intra-amniotic administration intestinal health BBM |
| url | https://www.mdpi.com/2072-6643/15/16/3643 |
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