Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell
Abstract The emergence of infectious diseases worldwide necessitates rapid and precise diagnostics. Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54406-0 |
| _version_ | 1797275187970834432 |
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| author | Jae Sung Ahn Woongkyu Park Do Hyun Jeong Soo Bong Choi Sun Hee Ahn |
| author_facet | Jae Sung Ahn Woongkyu Park Do Hyun Jeong Soo Bong Choi Sun Hee Ahn |
| author_sort | Jae Sung Ahn |
| collection | DOAJ |
| description | Abstract The emergence of infectious diseases worldwide necessitates rapid and precise diagnostics. Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profile. Our photothermal PCR method expedited DNA amplification while retaining its detection sensitivity. Combining photothermal quantitative PCR with real-time fluorometry and non-invasive temperature measurement, we could amplify the target DNA within just 25 min, with a minimum detectable DNA amount of 50 picograms. This innovation in photothermal qPCR, leveraging the photothermal properties of gold nanoshells, will pave the way for immediate point-of-care diagnostics of nucleic acid biomarkers. |
| first_indexed | 2024-03-07T15:09:45Z |
| format | Article |
| id | doaj.art-0aa72b8518e843e5951b48f135d16c41 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:09:45Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0aa72b8518e843e5951b48f135d16c412024-03-05T18:40:59ZengNature PortfolioScientific Reports2045-23222024-02-0114111010.1038/s41598-024-54406-0Rapid quantitative PCR equipment using photothermal conversion of Au nanoshellJae Sung Ahn0Woongkyu Park1Do Hyun Jeong2Soo Bong Choi3Sun Hee Ahn4Bio and Health Photonics Research Center, Korea Photonics Technology InstitutePhotonic Energy Components Research Center, Korea Photonics Technology InstituteBio and Health Photonics Research Center, Korea Photonics Technology InstituteDepartment of Physics, Incheon National UniversityBio and Health Photonics Research Center, Korea Photonics Technology InstituteAbstract The emergence of infectious diseases worldwide necessitates rapid and precise diagnostics. Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profile. Our photothermal PCR method expedited DNA amplification while retaining its detection sensitivity. Combining photothermal quantitative PCR with real-time fluorometry and non-invasive temperature measurement, we could amplify the target DNA within just 25 min, with a minimum detectable DNA amount of 50 picograms. This innovation in photothermal qPCR, leveraging the photothermal properties of gold nanoshells, will pave the way for immediate point-of-care diagnostics of nucleic acid biomarkers.https://doi.org/10.1038/s41598-024-54406-0 |
| spellingShingle | Jae Sung Ahn Woongkyu Park Do Hyun Jeong Soo Bong Choi Sun Hee Ahn Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell Scientific Reports |
| title | Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell |
| title_full | Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell |
| title_fullStr | Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell |
| title_full_unstemmed | Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell |
| title_short | Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell |
| title_sort | rapid quantitative pcr equipment using photothermal conversion of au nanoshell |
| url | https://doi.org/10.1038/s41598-024-54406-0 |
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