Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging
Many biomedical applications necessitate a targeted intracellular delivery of the nanomaterial to specific cells. Therefore, a non-invasive and reliable imaging tool is required to detect both the delivery and cellular endocytosis of the nanoparticles. Herein, we demonstrate that magneto-photo-acous...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-09-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597915300033 |
| _version_ | 1817997155843964928 |
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| author | M. Qu M. Mehrmohammadi S.Y. Emelianov |
| author_facet | M. Qu M. Mehrmohammadi S.Y. Emelianov |
| author_sort | M. Qu |
| collection | DOAJ |
| description | Many biomedical applications necessitate a targeted intracellular delivery of the nanomaterial to specific cells. Therefore, a non-invasive and reliable imaging tool is required to detect both the delivery and cellular endocytosis of the nanoparticles. Herein, we demonstrate that magneto-photo-acoustic (MPA) imaging can be used to monitor the delivery and to identify endocytosis of magnetic and optically absorbing nanoparticles. The relationship between photoacoustic (PA) and magneto-motive ultrasound (MMUS) signals from the in vitro samples were analyzed to identify the delivery and endocytosis of nanoparticles. The results indicated that during the delivery of nanoparticles to the vicinity of the cells, both PA and MMUS signals are almost linearly proportional. However, accumulation of nanoparticles within the cells leads to nonlinear MMUS-PA relationship, due to non-linear MMUS signal amplification. Therefore, through longitudinal MPA imaging, it is possible to monitor the delivery of nanoparticles and identify the endocytosis of the nanoparticles by living cells. |
| first_indexed | 2024-04-14T02:34:00Z |
| format | Article |
| id | doaj.art-0aa90a4e56584711a552b2478c5e25a7 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-14T02:34:00Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-0aa90a4e56584711a552b2478c5e25a72022-12-22T02:17:33ZengElsevierPhotoacoustics2213-59792015-09-013310711310.1016/j.pacs.2015.08.004Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imagingM. QuM. MehrmohammadiS.Y. EmelianovMany biomedical applications necessitate a targeted intracellular delivery of the nanomaterial to specific cells. Therefore, a non-invasive and reliable imaging tool is required to detect both the delivery and cellular endocytosis of the nanoparticles. Herein, we demonstrate that magneto-photo-acoustic (MPA) imaging can be used to monitor the delivery and to identify endocytosis of magnetic and optically absorbing nanoparticles. The relationship between photoacoustic (PA) and magneto-motive ultrasound (MMUS) signals from the in vitro samples were analyzed to identify the delivery and endocytosis of nanoparticles. The results indicated that during the delivery of nanoparticles to the vicinity of the cells, both PA and MMUS signals are almost linearly proportional. However, accumulation of nanoparticles within the cells leads to nonlinear MMUS-PA relationship, due to non-linear MMUS signal amplification. Therefore, through longitudinal MPA imaging, it is possible to monitor the delivery of nanoparticles and identify the endocytosis of the nanoparticles by living cells.http://www.sciencedirect.com/science/article/pii/S2213597915300033NanoparticlesEndocytosisDeliveryMagneto-photo-acoustic imaging |
| spellingShingle | M. Qu M. Mehrmohammadi S.Y. Emelianov Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging Photoacoustics Nanoparticles Endocytosis Delivery Magneto-photo-acoustic imaging |
| title | Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging |
| title_full | Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging |
| title_fullStr | Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging |
| title_full_unstemmed | Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging |
| title_short | Sensing the delivery and endocytosis of nanoparticles using magneto-photo-acoustic imaging |
| title_sort | sensing the delivery and endocytosis of nanoparticles using magneto photo acoustic imaging |
| topic | Nanoparticles Endocytosis Delivery Magneto-photo-acoustic imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2213597915300033 |
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