UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract
An effective ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF/MS) method was developed for analysing the chemical constituents in rat plasma and urine after the oral administration of Rubia cordifolia L. extract. Under the optimi...
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MDPI AG
2017-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/8/1327 |
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| author | Zuoliang Zheng Shengqing Li Yuping Zhong Ruoting Zhan Yan Yan Huafeng Pan Ping Yan |
| author_facet | Zuoliang Zheng Shengqing Li Yuping Zhong Ruoting Zhan Yan Yan Huafeng Pan Ping Yan |
| author_sort | Zuoliang Zheng |
| collection | DOAJ |
| description | An effective ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF/MS) method was developed for analysing the chemical constituents in rat plasma and urine after the oral administration of Rubia cordifolia L. extract. Under the optimized conditions, nine of 11 prototypes in rat plasma and four prototypes in urine were identified or characterized by comparing the retention time, accurate mass, fragmentation patterns, reference compounds, and literature data. In total, six metabolites, including alizarin-1-O-β-glucuronide, alizarin-2-O-β-glucuronide, alizarin-1-O-sulfation, alizarin-2-O-sulfation, purpurin-1-O-β-glucuronide, and purpurin-3-O-β-glucuronide, were identified in rat plasma, which were confirmed by lavaging standard solutions. Purpurin was found to be able to be transformed into alizarin based on the results in which alizarin was detected in rat plasma after the oral administration of a purpurin solution. In total, four metabolites were found in rat urine, but their chemical structures were not confirmed. The results indicate that the metabolic pathway of alizarin involves glucuronidation and sulfation, with the purpurins having undergone glucuronidation. The components absorbed into the blood, and the metabolites have the opportunity to become bioactive constituents. The experimental results would supply a helpful chemical basis for further research on the mechanism of actions of Rubia cordifolia L. |
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| language | English |
| last_indexed | 2024-12-22T02:28:10Z |
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| series | Molecules |
| spelling | doaj.art-0aab22981b8c4bffb537667b8cd855072022-12-21T18:41:57ZengMDPI AGMolecules1420-30492017-08-01228132710.3390/molecules22081327molecules22081327UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. ExtractZuoliang Zheng0Shengqing Li1Yuping Zhong2Ruoting Zhan3Yan Yan4Huafeng Pan5Ping Yan6Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaInstitute of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaInstitute of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaResearch Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaInstitute of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaGuangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaResearch Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, ChinaAn effective ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF/MS) method was developed for analysing the chemical constituents in rat plasma and urine after the oral administration of Rubia cordifolia L. extract. Under the optimized conditions, nine of 11 prototypes in rat plasma and four prototypes in urine were identified or characterized by comparing the retention time, accurate mass, fragmentation patterns, reference compounds, and literature data. In total, six metabolites, including alizarin-1-O-β-glucuronide, alizarin-2-O-β-glucuronide, alizarin-1-O-sulfation, alizarin-2-O-sulfation, purpurin-1-O-β-glucuronide, and purpurin-3-O-β-glucuronide, were identified in rat plasma, which were confirmed by lavaging standard solutions. Purpurin was found to be able to be transformed into alizarin based on the results in which alizarin was detected in rat plasma after the oral administration of a purpurin solution. In total, four metabolites were found in rat urine, but their chemical structures were not confirmed. The results indicate that the metabolic pathway of alizarin involves glucuronidation and sulfation, with the purpurins having undergone glucuronidation. The components absorbed into the blood, and the metabolites have the opportunity to become bioactive constituents. The experimental results would supply a helpful chemical basis for further research on the mechanism of actions of Rubia cordifolia L.https://www.mdpi.com/1420-3049/22/8/1327Rubia cordifolia L.UPLC/Q-TOF-MSmetabolitesalizarinpurpurin |
| spellingShingle | Zuoliang Zheng Shengqing Li Yuping Zhong Ruoting Zhan Yan Yan Huafeng Pan Ping Yan UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract Molecules Rubia cordifolia L. UPLC/Q-TOF-MS metabolites alizarin purpurin |
| title | UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract |
| title_full | UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract |
| title_fullStr | UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract |
| title_full_unstemmed | UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract |
| title_short | UPLC-QTOF-MS Identification of the Chemical Constituents in Rat Plasma and Urine after Oral Administration of Rubia cordifolia L. Extract |
| title_sort | uplc qtof ms identification of the chemical constituents in rat plasma and urine after oral administration of rubia cordifolia l extract |
| topic | Rubia cordifolia L. UPLC/Q-TOF-MS metabolites alizarin purpurin |
| url | https://www.mdpi.com/1420-3049/22/8/1327 |
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