Overcoming the Limitations of CRISPR-Cas9 Systems in <i>Saccharomyces cerevisiae</i>: Off-Target Effects, Epigenome, and Mitochondrial Editing

Overcoming the Limitations of CRISPR-Cas9 Systems in <i>Saccharomyces cerevisiae</i>: Off-Target Effects, Epigenome, and Mitochondrial Editing

Modification of the genome of the yeast <i>Saccharomyces cerevisiae</i> has great potential for application in biological research and biotechnological advancements, and the CRISPR-Cas9 system has been increasingly employed for these purposes. The CRISPR-Cas9 system enables the precise a...

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Bibliographic Details
Main Authors: Genki Sato, Kouichi Kuroda
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Microorganisms
Subjects:
<i>Saccharomyces cerevisiae</i>
genome editing
CRISPR-Cas9 system
CRISPR Nickase
epigenetics
mitochondrial DNA
Online Access:https://www.mdpi.com/2076-2607/11/4/1040

Internet

https://www.mdpi.com/2076-2607/11/4/1040

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