The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils
Symbiotic associations between leguminous plants and their nodule microbiome play a key role in sustainable agriculture by facilitating the fixation of atmospheric nitrogen and enhancing plant stress resistance. This study aimed to decipher the root nodule microbiome of two halophytic legumes, <i...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/2/207 |
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| author | Yanfen Zheng Jing Liang Dong-Lin Zhao Chen Meng Zong-Chang Xu Zhi-Hong Xie Cheng-Sheng Zhang |
| author_facet | Yanfen Zheng Jing Liang Dong-Lin Zhao Chen Meng Zong-Chang Xu Zhi-Hong Xie Cheng-Sheng Zhang |
| author_sort | Yanfen Zheng |
| collection | DOAJ |
| description | Symbiotic associations between leguminous plants and their nodule microbiome play a key role in sustainable agriculture by facilitating the fixation of atmospheric nitrogen and enhancing plant stress resistance. This study aimed to decipher the root nodule microbiome of two halophytic legumes, <i>Sesbania cannabina</i> and <i>Glycine soja</i>, which grow in saline soils of the Yellow River Delta, China, using PacBio’s circular consensus sequencing for full-length bacterial 16S rRNA gene to obtain finer taxonomic information. The cultivated legume <i>Glycine max</i> was used for comparison. We identified 18 bacterial genera and 55 species in nodule samples, which mainly classified to <i>Proteobacteria</i>, and rhizobial genus <i>Ensifer</i> was the predominant group. The three legumes showed similarity in operational taxonomic unit (OTU) diversity but distinction in OTU richness, indicating that they harbor similar bacterial species with different relative contents. The results of principal coordinates analysis and ANOSIM tests indicated that <i>G. soja</i> and <i>G. max</i> have similar nodule bacterial communities, and these communities differ from that of <i>S. cannabina</i>. Wild legumes <i>S. cannabina</i> and <i>G. soja</i> both harbored a higher number of rhizobia, while <i>G. max</i> possessed more non-rhizobial bacteria. These differences could be associated with their adaptability to saline−alkali stress and revealed clues on the nodule endophytes with relative importance of culturable rhizobial symbionts. |
| first_indexed | 2024-12-11T05:51:00Z |
| format | Article |
| id | doaj.art-0aafd0f313594fd7b682b885431811bf |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T05:51:00Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0aafd0f313594fd7b682b885431811bf2022-12-22T01:18:49ZengMDPI AGMicroorganisms2076-26072020-02-018220710.3390/microorganisms8020207microorganisms8020207The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline SoilsYanfen Zheng0Jing Liang1Dong-Lin Zhao2Chen Meng3Zong-Chang Xu4Zhi-Hong Xie5Cheng-Sheng Zhang6Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaMarine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaMarine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaMarine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaMarine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaMarine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaSymbiotic associations between leguminous plants and their nodule microbiome play a key role in sustainable agriculture by facilitating the fixation of atmospheric nitrogen and enhancing plant stress resistance. This study aimed to decipher the root nodule microbiome of two halophytic legumes, <i>Sesbania cannabina</i> and <i>Glycine soja</i>, which grow in saline soils of the Yellow River Delta, China, using PacBio’s circular consensus sequencing for full-length bacterial 16S rRNA gene to obtain finer taxonomic information. The cultivated legume <i>Glycine max</i> was used for comparison. We identified 18 bacterial genera and 55 species in nodule samples, which mainly classified to <i>Proteobacteria</i>, and rhizobial genus <i>Ensifer</i> was the predominant group. The three legumes showed similarity in operational taxonomic unit (OTU) diversity but distinction in OTU richness, indicating that they harbor similar bacterial species with different relative contents. The results of principal coordinates analysis and ANOSIM tests indicated that <i>G. soja</i> and <i>G. max</i> have similar nodule bacterial communities, and these communities differ from that of <i>S. cannabina</i>. Wild legumes <i>S. cannabina</i> and <i>G. soja</i> both harbored a higher number of rhizobia, while <i>G. max</i> possessed more non-rhizobial bacteria. These differences could be associated with their adaptability to saline−alkali stress and revealed clues on the nodule endophytes with relative importance of culturable rhizobial symbionts.https://www.mdpi.com/2076-2607/8/2/207nodule microbiomehalophytic legume<i>sesbania cannabina</i><i>glycine soja</i>16s rdna full-length amplicon sequencing |
| spellingShingle | Yanfen Zheng Jing Liang Dong-Lin Zhao Chen Meng Zong-Chang Xu Zhi-Hong Xie Cheng-Sheng Zhang The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils Microorganisms nodule microbiome halophytic legume <i>sesbania cannabina</i> <i>glycine soja</i> 16s rdna full-length amplicon sequencing |
| title | The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils |
| title_full | The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils |
| title_fullStr | The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils |
| title_full_unstemmed | The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils |
| title_short | The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils |
| title_sort | root nodule microbiome of cultivated and wild halophytic legumes showed similar diversity but distinct community structure in yellow river delta saline soils |
| topic | nodule microbiome halophytic legume <i>sesbania cannabina</i> <i>glycine soja</i> 16s rdna full-length amplicon sequencing |
| url | https://www.mdpi.com/2076-2607/8/2/207 |
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