Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study.
<h4>Unlabelled</h4>The aim of the present work was to test the potential of Proton Transfer Reaction Time-of-Flight Mass Spectrometry (PTR-ToF-MS) in the diagnosis of liver cirrhosis and the assessment of disease severity by direct analysis of exhaled breath. Twenty-six volunteers have b...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573204/?tool=EBI |
| _version_ | 1818700120894472192 |
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| author | Filomena Morisco Eugenio Aprea Vincenzo Lembo Vincenzo Fogliano Paola Vitaglione Giovanna Mazzone Luca Cappellin Flavia Gasperi Stefania Masone Giovanni Domenico De Palma Riccardo Marmo Nicola Caporaso Franco Biasioli |
| author_facet | Filomena Morisco Eugenio Aprea Vincenzo Lembo Vincenzo Fogliano Paola Vitaglione Giovanna Mazzone Luca Cappellin Flavia Gasperi Stefania Masone Giovanni Domenico De Palma Riccardo Marmo Nicola Caporaso Franco Biasioli |
| author_sort | Filomena Morisco |
| collection | DOAJ |
| description | <h4>Unlabelled</h4>The aim of the present work was to test the potential of Proton Transfer Reaction Time-of-Flight Mass Spectrometry (PTR-ToF-MS) in the diagnosis of liver cirrhosis and the assessment of disease severity by direct analysis of exhaled breath. Twenty-six volunteers have been enrolled in this study: 12 patients (M/F 8/4, mean age 70.5 years, min-max 42-80 years) with liver cirrhosis of different etiologies and at different severity of disease and 14 healthy subjects (M/F 5/9, mean age 52.3 years, min-max 35-77 years). Real time breath analysis was performed on fasting subjects using a buffered end-tidal on-line sampler directly coupled to a PTR-ToF-MS. Twelve volatile organic compounds (VOCs) resulted significantly differently in cirrhotic patients (CP) compared to healthy controls (CTRL): four ketones (2-butanone, 2- or 3- pentanone, C8-ketone, C9-ketone), two terpenes (monoterpene, monoterpene related), four sulphur or nitrogen compounds (sulfoxide-compound, S-compound, NS-compound, N-compound) and two alcohols (heptadienol, methanol). Seven VOCs (2-butanone, C8-ketone, a monoterpene, 2,4-heptadienol and three compounds containing N, S or NS) resulted significantly differently in compensate cirrhotic patients (Child-Pugh A; CP-A) and decompensated cirrhotic subjects (Child-Pugh B+C; CP-B+C). ROC (Receiver Operating Characteristic) analysis was performed considering three contrast groups: CP vs CTRL, CP-A vs CTRL and CP-A vs CP-B+C. In these comparisons monoterpene and N-compound showed the best diagnostic performance.<h4>Conclusions</h4>Breath analysis by PTR-ToF-MS was able to distinguish cirrhotic patients from healthy subjects and to discriminate those with well compensated liver disease from those at more advanced severity stage. A breath-print of liver cirrhosis was assessed for the first time. |
| first_indexed | 2024-12-17T14:59:54Z |
| format | Article |
| id | doaj.art-0ab0e68405cd43d68a61337cf1325b33 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T14:59:54Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0ab0e68405cd43d68a61337cf1325b332022-12-21T21:43:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e5965810.1371/journal.pone.0059658Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study.Filomena MoriscoEugenio ApreaVincenzo LemboVincenzo FoglianoPaola VitaglioneGiovanna MazzoneLuca CappellinFlavia GasperiStefania MasoneGiovanni Domenico De PalmaRiccardo MarmoNicola CaporasoFranco Biasioli<h4>Unlabelled</h4>The aim of the present work was to test the potential of Proton Transfer Reaction Time-of-Flight Mass Spectrometry (PTR-ToF-MS) in the diagnosis of liver cirrhosis and the assessment of disease severity by direct analysis of exhaled breath. Twenty-six volunteers have been enrolled in this study: 12 patients (M/F 8/4, mean age 70.5 years, min-max 42-80 years) with liver cirrhosis of different etiologies and at different severity of disease and 14 healthy subjects (M/F 5/9, mean age 52.3 years, min-max 35-77 years). Real time breath analysis was performed on fasting subjects using a buffered end-tidal on-line sampler directly coupled to a PTR-ToF-MS. Twelve volatile organic compounds (VOCs) resulted significantly differently in cirrhotic patients (CP) compared to healthy controls (CTRL): four ketones (2-butanone, 2- or 3- pentanone, C8-ketone, C9-ketone), two terpenes (monoterpene, monoterpene related), four sulphur or nitrogen compounds (sulfoxide-compound, S-compound, NS-compound, N-compound) and two alcohols (heptadienol, methanol). Seven VOCs (2-butanone, C8-ketone, a monoterpene, 2,4-heptadienol and three compounds containing N, S or NS) resulted significantly differently in compensate cirrhotic patients (Child-Pugh A; CP-A) and decompensated cirrhotic subjects (Child-Pugh B+C; CP-B+C). ROC (Receiver Operating Characteristic) analysis was performed considering three contrast groups: CP vs CTRL, CP-A vs CTRL and CP-A vs CP-B+C. In these comparisons monoterpene and N-compound showed the best diagnostic performance.<h4>Conclusions</h4>Breath analysis by PTR-ToF-MS was able to distinguish cirrhotic patients from healthy subjects and to discriminate those with well compensated liver disease from those at more advanced severity stage. A breath-print of liver cirrhosis was assessed for the first time.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573204/?tool=EBI |
| spellingShingle | Filomena Morisco Eugenio Aprea Vincenzo Lembo Vincenzo Fogliano Paola Vitaglione Giovanna Mazzone Luca Cappellin Flavia Gasperi Stefania Masone Giovanni Domenico De Palma Riccardo Marmo Nicola Caporaso Franco Biasioli Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. PLoS ONE |
| title | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. |
| title_full | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. |
| title_fullStr | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. |
| title_full_unstemmed | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. |
| title_short | Rapid "breath-print" of liver cirrhosis by proton transfer reaction time-of-flight mass spectrometry. A pilot study. |
| title_sort | rapid breath print of liver cirrhosis by proton transfer reaction time of flight mass spectrometry a pilot study |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573204/?tool=EBI |
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