Menthol binding and inhibition of α7-nicotinic acetylcholine receptors.
Menthol is a common compound in pharmaceutical and commercial products and a popular additive to cigarettes. The molecular targets of menthol remain poorly defined. In this study we show an effect of menthol on the α7 subunit of the nicotinic acetylcholine (nACh) receptor function. Using a two-elect...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935840/pdf/?tool=EBI |
| _version_ | 1818573265546772480 |
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| author | Abrar Ashoor Jacob C Nordman Daniel Veltri Keun-Hang Susan Yang Lina Al Kury Yaroslav Shuba Mohamed Mahgoub Frank C Howarth Bassem Sadek Amarda Shehu Nadine Kabbani Murat Oz |
| author_facet | Abrar Ashoor Jacob C Nordman Daniel Veltri Keun-Hang Susan Yang Lina Al Kury Yaroslav Shuba Mohamed Mahgoub Frank C Howarth Bassem Sadek Amarda Shehu Nadine Kabbani Murat Oz |
| author_sort | Abrar Ashoor |
| collection | DOAJ |
| description | Menthol is a common compound in pharmaceutical and commercial products and a popular additive to cigarettes. The molecular targets of menthol remain poorly defined. In this study we show an effect of menthol on the α7 subunit of the nicotinic acetylcholine (nACh) receptor function. Using a two-electrode voltage-clamp technique, menthol was found to reversibly inhibit α7-nACh receptors heterologously expressed in Xenopus oocytes. Inhibition by menthol was not dependent on the membrane potential and did not involve endogenous Ca(2+)-dependent Cl(-) channels, since menthol inhibition remained unchanged by intracellular injection of the Ca(2+) chelator BAPTA and perfusion with Ca(2+)-free bathing solution containing Ba(2+). Furthermore, increasing ACh concentrations did not reverse menthol inhibition and the specific binding of [(125)I] α-bungarotoxin was not attenuated by menthol. Studies of α7- nACh receptors endogenously expressed in neural cells demonstrate that menthol attenuates α7 mediated Ca(2+) transients in the cell body and neurite. In conclusion, our results suggest that menthol inhibits α7-nACh receptors in a noncompetitive manner. |
| first_indexed | 2024-12-15T00:08:53Z |
| format | Article |
| id | doaj.art-0ab3f1966f7b4ab58c4106bf63823f1b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-15T00:08:53Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0ab3f1966f7b4ab58c4106bf63823f1b2022-12-21T22:42:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6767410.1371/journal.pone.0067674Menthol binding and inhibition of α7-nicotinic acetylcholine receptors.Abrar AshoorJacob C NordmanDaniel VeltriKeun-Hang Susan YangLina Al KuryYaroslav ShubaMohamed MahgoubFrank C HowarthBassem SadekAmarda ShehuNadine KabbaniMurat OzMenthol is a common compound in pharmaceutical and commercial products and a popular additive to cigarettes. The molecular targets of menthol remain poorly defined. In this study we show an effect of menthol on the α7 subunit of the nicotinic acetylcholine (nACh) receptor function. Using a two-electrode voltage-clamp technique, menthol was found to reversibly inhibit α7-nACh receptors heterologously expressed in Xenopus oocytes. Inhibition by menthol was not dependent on the membrane potential and did not involve endogenous Ca(2+)-dependent Cl(-) channels, since menthol inhibition remained unchanged by intracellular injection of the Ca(2+) chelator BAPTA and perfusion with Ca(2+)-free bathing solution containing Ba(2+). Furthermore, increasing ACh concentrations did not reverse menthol inhibition and the specific binding of [(125)I] α-bungarotoxin was not attenuated by menthol. Studies of α7- nACh receptors endogenously expressed in neural cells demonstrate that menthol attenuates α7 mediated Ca(2+) transients in the cell body and neurite. In conclusion, our results suggest that menthol inhibits α7-nACh receptors in a noncompetitive manner.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935840/pdf/?tool=EBI |
| spellingShingle | Abrar Ashoor Jacob C Nordman Daniel Veltri Keun-Hang Susan Yang Lina Al Kury Yaroslav Shuba Mohamed Mahgoub Frank C Howarth Bassem Sadek Amarda Shehu Nadine Kabbani Murat Oz Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. PLoS ONE |
| title | Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. |
| title_full | Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. |
| title_fullStr | Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. |
| title_full_unstemmed | Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. |
| title_short | Menthol binding and inhibition of α7-nicotinic acetylcholine receptors. |
| title_sort | menthol binding and inhibition of α7 nicotinic acetylcholine receptors |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935840/pdf/?tool=EBI |
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