Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions
At the end of the 1920s, Vavilov organized several potato-collecting missions in South and Central America. Vavilov and his colleagues, Juzepczuk and Bukasov, participated in these expeditions and worked on gathered material, designated two centers of potato varietal riches and diversity—the Peru–Bo...
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MDPI AG
2022-12-01
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| author | Tatjana Gavrilenko Irena Chukhina Olga Antonova Ekaterina Krylova Liliya Shipilina Natalia Oskina Ludmila Kostina |
| author_facet | Tatjana Gavrilenko Irena Chukhina Olga Antonova Ekaterina Krylova Liliya Shipilina Natalia Oskina Ludmila Kostina |
| author_sort | Tatjana Gavrilenko |
| collection | DOAJ |
| description | At the end of the 1920s, Vavilov organized several potato-collecting missions in South and Central America. Vavilov and his colleagues, Juzepczuk and Bukasov, participated in these expeditions and worked on gathered material, designated two centers of potato varietal riches and diversity—the Peru–Bolivia high-mountain center and the southern coast of Chile. The WIR Herbarium holds authentic specimens of many taxa described by Russian taxonomists. Here, a set of 20 plastid DNA-specific markers was applied for 49 authentic herbarium specimens of <i>Solanum tuberosum</i> L. from the WIR Herbarium to analyze the genetic diversity of the landrace population collected by Juzepczuk in 1928 in southern–central Chile. Two plastid DNA types, T and A, and two chlorotypes were identified in herbarium specimens, with a clear predominance (96%) of chlorotype cpT_III. In addition, we analyzed 46 living Chilean accessions from the VIR field potato gene bank that were collected after the appearance of <i>Phytophthora infestans</i> in Chile. These living accessions were differentiated into four chlorotypes. Finding a D-type cytoplasm in living Chilean accessions that possess two new chlorotypes indicates a replacement of native cultivars and introgression from the wild Mexican species <i>S. demissum</i> that was actively used in breeding as a source of race-specific resistance to late blight. |
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| id | doaj.art-0ab4797f36ee4850adb7b6b1abab4b02 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T12:01:19Z |
| publishDate | 2022-12-01 |
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| series | Plants |
| spelling | doaj.art-0ab4797f36ee4850adb7b6b1abab4b022023-11-30T23:02:59ZengMDPI AGPlants2223-77472022-12-0112117410.3390/plants12010174Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank AccessionsTatjana Gavrilenko0Irena Chukhina1Olga Antonova2Ekaterina Krylova3Liliya Shipilina4Natalia Oskina5Ludmila Kostina6N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya 42-44, 190000 Saint-Petersburg, RussiaAt the end of the 1920s, Vavilov organized several potato-collecting missions in South and Central America. Vavilov and his colleagues, Juzepczuk and Bukasov, participated in these expeditions and worked on gathered material, designated two centers of potato varietal riches and diversity—the Peru–Bolivia high-mountain center and the southern coast of Chile. The WIR Herbarium holds authentic specimens of many taxa described by Russian taxonomists. Here, a set of 20 plastid DNA-specific markers was applied for 49 authentic herbarium specimens of <i>Solanum tuberosum</i> L. from the WIR Herbarium to analyze the genetic diversity of the landrace population collected by Juzepczuk in 1928 in southern–central Chile. Two plastid DNA types, T and A, and two chlorotypes were identified in herbarium specimens, with a clear predominance (96%) of chlorotype cpT_III. In addition, we analyzed 46 living Chilean accessions from the VIR field potato gene bank that were collected after the appearance of <i>Phytophthora infestans</i> in Chile. These living accessions were differentiated into four chlorotypes. Finding a D-type cytoplasm in living Chilean accessions that possess two new chlorotypes indicates a replacement of native cultivars and introgression from the wild Mexican species <i>S. demissum</i> that was actively used in breeding as a source of race-specific resistance to late blight.https://www.mdpi.com/2223-7747/12/1/174<i>Solanum tuberosum</i>cytoplasmic genetic diversityherbarium specimenspotato germplasm collectionsplastid DNA haplotypesmicrosatellites |
| spellingShingle | Tatjana Gavrilenko Irena Chukhina Olga Antonova Ekaterina Krylova Liliya Shipilina Natalia Oskina Ludmila Kostina Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions Plants <i>Solanum tuberosum</i> cytoplasmic genetic diversity herbarium specimens potato germplasm collections plastid DNA haplotypes microsatellites |
| title | Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions |
| title_full | Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions |
| title_fullStr | Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions |
| title_full_unstemmed | Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions |
| title_short | Comparative Analysis of the Genetic Diversity of Chilean Cultivated Potato Based on a Molecular Study of Authentic Herbarium Specimens and Present-Day Gene Bank Accessions |
| title_sort | comparative analysis of the genetic diversity of chilean cultivated potato based on a molecular study of authentic herbarium specimens and present day gene bank accessions |
| topic | <i>Solanum tuberosum</i> cytoplasmic genetic diversity herbarium specimens potato germplasm collections plastid DNA haplotypes microsatellites |
| url | https://www.mdpi.com/2223-7747/12/1/174 |
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