Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling
Summary: This protocol allows repeated whole-cell patch-clamp recordings from individual rodent CA1 hippocampal neurons, followed by immunohistological labeling of the axon initial segment. This overcomes the need to maintain whole-cell recordings over the timescales required for homeostatic modific...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000435 |
| _version_ | 1819163655378305024 |
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| author | Laura S. Oliveira Anna Sumera Sam A. Booker |
| author_facet | Laura S. Oliveira Anna Sumera Sam A. Booker |
| author_sort | Laura S. Oliveira |
| collection | DOAJ |
| description | Summary: This protocol allows repeated whole-cell patch-clamp recordings from individual rodent CA1 hippocampal neurons, followed by immunohistological labeling of the axon initial segment. This overcomes the need to maintain whole-cell recordings over the timescales required for homeostatic modification to cellular excitability, allowing for correlative analysis of the structure and function of neurons. Moreover, this protocol allows for paired analysis of physiological properties assessed before and after pharmacological treatment, thus providing increased statistical power, despite the relatively low-throughput nature of the recordings.For complete details on the use and execution of this protocol, please refer to Booker et al. (2020a). |
| first_indexed | 2024-12-22T17:47:35Z |
| format | Article |
| id | doaj.art-0ab4d295f9524e6fa06ea36e67fa033b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T17:47:35Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0ab4d295f9524e6fa06ea36e67fa033b2022-12-21T18:18:15ZengElsevierSTAR Protocols2666-16672021-03-0121100336Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labelingLaura S. Oliveira0Anna Sumera1Sam A. Booker2Simons Initiative for the Developing Brain, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Patrick Wild Centre for Research into Autism, Fragile X Syndome, and Intellectual Disability, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Corresponding authorSimons Initiative for the Developing Brain, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Patrick Wild Centre for Research into Autism, Fragile X Syndome, and Intellectual Disability, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UKSimons Initiative for the Developing Brain, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Patrick Wild Centre for Research into Autism, Fragile X Syndome, and Intellectual Disability, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Centre for Discovery Brain Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK; Corresponding authorSummary: This protocol allows repeated whole-cell patch-clamp recordings from individual rodent CA1 hippocampal neurons, followed by immunohistological labeling of the axon initial segment. This overcomes the need to maintain whole-cell recordings over the timescales required for homeostatic modification to cellular excitability, allowing for correlative analysis of the structure and function of neurons. Moreover, this protocol allows for paired analysis of physiological properties assessed before and after pharmacological treatment, thus providing increased statistical power, despite the relatively low-throughput nature of the recordings.For complete details on the use and execution of this protocol, please refer to Booker et al. (2020a).http://www.sciencedirect.com/science/article/pii/S2666166721000435Single cellMicroscopyNeuroscience |
| spellingShingle | Laura S. Oliveira Anna Sumera Sam A. Booker Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling STAR Protocols Single cell Microscopy Neuroscience |
| title | Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| title_full | Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| title_fullStr | Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| title_full_unstemmed | Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| title_short | Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| title_sort | repeated whole cell patch clamp recording from ca1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling |
| topic | Single cell Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000435 |
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