Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin
Molecular markers are a helpful tool for species detection of meat origin, in order to determine with accuracy the possible fraud of animal-based food products. In this study, blood, milk and cheese samples were taken from the Romanian “Bălțata” bovine species. In the blood extraction stage, the com...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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AcademicPres
2020-05-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| Subjects: | |
| Online Access: | https://journals.usamvcluj.ro/index.php/fst/article/view/13723 |
| _version_ | 1818279652635967488 |
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| author | Lavinia Maria CHIŞ Dan Cristian VODNAR |
| author_facet | Lavinia Maria CHIŞ Dan Cristian VODNAR |
| author_sort | Lavinia Maria CHIŞ |
| collection | DOAJ |
| description | Molecular markers are a helpful tool for species detection of meat origin, in order to determine with accuracy the possible fraud of animal-based food products. In this study, blood, milk and cheese samples were taken from the Romanian “Bălțata” bovine species. In the blood extraction stage, the commercial Wizard Genomic DNA Extraction Kit was used, and for the milk and cheese extraction, the SureFood®PREP Advanced kit was used. Target DNA amplification in all the three matrices was performed with the StockMarks for Cattle kit. It was found that the analyzed loci had the size of the fragments within the reference range given by the kit used, which concludes that the blood, milk and the cheese have the same origin, from the same animal of the bovine species. Therefore, through the genotyping technique, traceability of food products can be achieved and the species of origin can be identified. |
| first_indexed | 2024-12-12T23:36:44Z |
| format | Article |
| id | doaj.art-0ab5d5b645dc4852818db852c7acf784 |
| institution | Directory Open Access Journal |
| issn | 2344-2344 2344-5300 |
| language | English |
| last_indexed | 2024-12-12T23:36:44Z |
| publishDate | 2020-05-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| spelling | doaj.art-0ab5d5b645dc4852818db852c7acf7842022-12-22T00:07:26ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002020-05-01771627310.15835/buasvmcn-fst:2019.003511196Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal OriginLavinia Maria CHIŞ0Dan Cristian VODNAR1Life Science Institute, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Calea Mănăştur Street, Cluj-Napoca, 400372, RomaniaLife Science Institute, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 Calea Mănăştur Street, Cluj-Napoca, 400372, RomaniaMolecular markers are a helpful tool for species detection of meat origin, in order to determine with accuracy the possible fraud of animal-based food products. In this study, blood, milk and cheese samples were taken from the Romanian “Bălțata” bovine species. In the blood extraction stage, the commercial Wizard Genomic DNA Extraction Kit was used, and for the milk and cheese extraction, the SureFood®PREP Advanced kit was used. Target DNA amplification in all the three matrices was performed with the StockMarks for Cattle kit. It was found that the analyzed loci had the size of the fragments within the reference range given by the kit used, which concludes that the blood, milk and the cheese have the same origin, from the same animal of the bovine species. Therefore, through the genotyping technique, traceability of food products can be achieved and the species of origin can be identified.https://journals.usamvcluj.ro/index.php/fst/article/view/13723dna markersgenotypingtraceabilitypcr multiplexbovine. |
| spellingShingle | Lavinia Maria CHIŞ Dan Cristian VODNAR Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology dna markers genotyping traceability pcr multiplex bovine. |
| title | Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin |
| title_full | Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin |
| title_fullStr | Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin |
| title_full_unstemmed | Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin |
| title_short | Identification of Molecular Markers Specific to the Bovine Species Used in the Production of Food Products of Animal Origin |
| title_sort | identification of molecular markers specific to the bovine species used in the production of food products of animal origin |
| topic | dna markers genotyping traceability pcr multiplex bovine. |
| url | https://journals.usamvcluj.ro/index.php/fst/article/view/13723 |
| work_keys_str_mv | AT laviniamariachis identificationofmolecularmarkersspecifictothebovinespeciesusedintheproductionoffoodproductsofanimalorigin AT dancristianvodnar identificationofmolecularmarkersspecifictothebovinespeciesusedintheproductionoffoodproductsofanimalorigin |