The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer

Abstract Background Research has shown that some microbiomes are linked to cancer. Hence, we hypothesize that alterations in the respiratory microbiome might be associated with lung cancer. Methods Through droplet digital polymerase chain reaction analysis, we investigated the abundance of Acidovora...

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Main Authors: Masamitsu Shimizu, Akihiko Miyanaga, Masahiro Seike, Kuniko Matsuda, Masaru Matsumoto, Rintaro Noro, Kazue Fujita, Yoko Mano, Nobuhiko Furuya, Kaoru Kubota, Akihiko Gemma
Format: Article
Language:English
Published: Wiley 2022-07-01
Series:Thoracic Cancer
Subjects:
Online Access:https://doi.org/10.1111/1759-7714.14463
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author Masamitsu Shimizu
Akihiko Miyanaga
Masahiro Seike
Kuniko Matsuda
Masaru Matsumoto
Rintaro Noro
Kazue Fujita
Yoko Mano
Nobuhiko Furuya
Kaoru Kubota
Akihiko Gemma
author_facet Masamitsu Shimizu
Akihiko Miyanaga
Masahiro Seike
Kuniko Matsuda
Masaru Matsumoto
Rintaro Noro
Kazue Fujita
Yoko Mano
Nobuhiko Furuya
Kaoru Kubota
Akihiko Gemma
author_sort Masamitsu Shimizu
collection DOAJ
description Abstract Background Research has shown that some microbiomes are linked to cancer. Hence, we hypothesize that alterations in the respiratory microbiome might be associated with lung cancer. Methods Through droplet digital polymerase chain reaction analysis, we investigated the abundance of Acidovorax in surgically resected primary tumors and corresponding nontumor lung tissues obtained from 50 Japanese patients with non‐small cell lung cancer. Results The rate of positivity for Acidovorax in tumor and nontumor tissues was 44 and 26%, respectively. The abundance of Acidovorax in tumor tissues was significantly higher in patients with nonsquamous cell carcinoma complicated by chronic obstructive pulmonary disease (COPD) and those who relapsed after surgical resection (p < 0.05). In tumor tissues, the results of the univariate and multivariate analyses revealed that only COPD exerted a direct effect on the abundance of Acidovorax (p < 0.05). Furthermore, the presence of Acidovorax was high in lung cancer patients with COPD comorbidity (65%) and TP53 gene mutation; only one of the nontumor tissues was positive for Acidovorax. In patients with lung cancer complicated by COPD, Acidovorax tended to be present in both the tumor and nontumor areas. Conclusions This study identified novel microbiota involved in lung cancer with COPD comorbidity. The results suggested that Acidovorax may be a useful biomarker in the screening for lung cancer. Further studies are warranted to validate the clinical significance of the microbiome in a larger independent patient cohort.
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spelling doaj.art-0ab633eded5342b0b12ea89922f120f82022-12-22T02:28:29ZengWileyThoracic Cancer1759-77061759-77142022-07-0113131940194710.1111/1759-7714.14463The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancerMasamitsu Shimizu0Akihiko Miyanaga1Masahiro Seike2Kuniko Matsuda3Masaru Matsumoto4Rintaro Noro5Kazue Fujita6Yoko Mano7Nobuhiko Furuya8Kaoru Kubota9Akihiko Gemma10Department of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Clinical Laboratory Medicine, Faculty of Health Science Technology Bunkyo Gakuin University Tokyo JapanDepartment of Clinical Laboratory Medicine, Faculty of Health Science Technology Bunkyo Gakuin University Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanDepartment of Pulmonary Medicine and Oncology, Graduate School of Medicine Nippon Medical School Tokyo JapanAbstract Background Research has shown that some microbiomes are linked to cancer. Hence, we hypothesize that alterations in the respiratory microbiome might be associated with lung cancer. Methods Through droplet digital polymerase chain reaction analysis, we investigated the abundance of Acidovorax in surgically resected primary tumors and corresponding nontumor lung tissues obtained from 50 Japanese patients with non‐small cell lung cancer. Results The rate of positivity for Acidovorax in tumor and nontumor tissues was 44 and 26%, respectively. The abundance of Acidovorax in tumor tissues was significantly higher in patients with nonsquamous cell carcinoma complicated by chronic obstructive pulmonary disease (COPD) and those who relapsed after surgical resection (p < 0.05). In tumor tissues, the results of the univariate and multivariate analyses revealed that only COPD exerted a direct effect on the abundance of Acidovorax (p < 0.05). Furthermore, the presence of Acidovorax was high in lung cancer patients with COPD comorbidity (65%) and TP53 gene mutation; only one of the nontumor tissues was positive for Acidovorax. In patients with lung cancer complicated by COPD, Acidovorax tended to be present in both the tumor and nontumor areas. Conclusions This study identified novel microbiota involved in lung cancer with COPD comorbidity. The results suggested that Acidovorax may be a useful biomarker in the screening for lung cancer. Further studies are warranted to validate the clinical significance of the microbiome in a larger independent patient cohort.https://doi.org/10.1111/1759-7714.14463COPDddPCRlung cancermicrobiome
spellingShingle Masamitsu Shimizu
Akihiko Miyanaga
Masahiro Seike
Kuniko Matsuda
Masaru Matsumoto
Rintaro Noro
Kazue Fujita
Yoko Mano
Nobuhiko Furuya
Kaoru Kubota
Akihiko Gemma
The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
Thoracic Cancer
COPD
ddPCR
lung cancer
microbiome
title The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
title_full The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
title_fullStr The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
title_full_unstemmed The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
title_short The respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non‐small cell lung cancer
title_sort respiratory microbiome associated with chronic obstructive pulmonary disease comorbidity in non small cell lung cancer
topic COPD
ddPCR
lung cancer
microbiome
url https://doi.org/10.1111/1759-7714.14463
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