FOXD2-AS1 inhibits the proliferation and migration in prostate cancer: an in vitro and in vivo study
FOXD2 Adjacent Opposite Strand RNA 1 (FOXD2-AS1), a long noncoding RNA (lncRNA), exhibits specifically elevated in numerous cancerous cells. Numerous studies have shown that FOXD2-AS1 encourages cellular proliferation, migration and invasion. Nevertheless, the exact mechanism through which FOXD2-...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MRE Press
2023-09-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/files/article/20230928-106/pdf/JOMH2023070601.pdf |
| _version_ | 1797370261025062912 |
|---|---|
| author | Xiong Mei Yongli Nie Jun Chen Wei Wang |
| author_facet | Xiong Mei Yongli Nie Jun Chen Wei Wang |
| author_sort | Xiong Mei |
| collection | DOAJ |
| description | FOXD2 Adjacent Opposite Strand RNA 1 (FOXD2-AS1), a long
noncoding RNA (lncRNA), exhibits specifically elevated in numerous cancerous
cells. Numerous studies have shown that FOXD2-AS1 encourages cellular proliferation, migration and invasion.
Nevertheless, the exact mechanism through which FOXD2-AS1 contributes to
prostate cancer (PCa) remains unclear. Consequently, we aimed to explore the
implications of FOXD2-AS1 on the growth of PCa.
Initially, an elevation of FOXD2-AS1 observed in PCa cells (PC-3,
DU145 and Lncap) than the prostate normal cell line RWPE2. Then, PC-3 cells were tranafected with shFOXD2-AS1,
sh-Numerical Control (shNC) or FOXD2-AS1 to assess the implications of FOXD2-AS. Cell growth
was measured with cell counting kit-8 (CCK8) and 5-ethynyl-2′-deoxyuridine (EDU)
assays, and cell invasion and migration were assessed by Transwell assays, which
demonstrated that FOXD2-AS1 silence impeded proliferation, migration and
invasion of PC-3 cells. Additionally, we discovered that FOXD2-AS1
bonded with miR-206/programmed cell death protein 10 (PDCD10) trough analyzing the interaction sites of lncRNA,
miRNA and protein. Then, these interaction abilities were confirmed by
dual-luciferase reporter assays and RT-qPCR, suggesting FOXD2-AS1 could
upregulate the amount of PDCD10 through suppressing miR-206. Furthermore, the
role of FOXD2-AS1 silencing on PCa carcinogenesis were assessed.
In vivo experiment, shFOXD2-AS1 led to a notable reduction in both the size and weight of
PCa. These findings indicated that FOXD2-AS1 silencing effectively
hindered the progression of prostate cancer.
In conclusion, the upregulation of FOXD2-AS1 was
observed in PCa, and the knockdown of FOXD2-AS1 could alleviated tumor
development by targeting miR-206 to upregulate PDCD10 expression. |
| first_indexed | 2024-03-08T17:59:48Z |
| format | Article |
| id | doaj.art-0abef0f1aa154612a7815d74161afe96 |
| institution | Directory Open Access Journal |
| issn | 1875-6867 1875-6859 |
| language | English |
| last_indexed | 2024-03-08T17:59:48Z |
| publishDate | 2023-09-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj.art-0abef0f1aa154612a7815d74161afe962024-01-02T02:27:46ZengMRE PressJournal of Men's Health1875-68671875-68592023-09-0119911912610.22514/jomh.2023.092S1875-6867(23)00061-1FOXD2-AS1 inhibits the proliferation and migration in prostate cancer: an in vitro and in vivo studyXiong Mei0Yongli Nie1Jun Chen2Wei Wang3Department of Oncology, Sinopharm Han Jiang Hospital, 442700 Shiyan, Hubei, ChinaDepartment of Oncology, Sinopharm Han Jiang Hospital, 442700 Shiyan, Hubei, ChinaExperimental center, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, 442008 Shiyan, Hubei, ChinaDepartment of Medical Laboratory, Chongqing University Cancer Hospital, Chongqing Cancer Institute, Chongqing Cancer Hospital, 400010 Chongqing, ChinaFOXD2 Adjacent Opposite Strand RNA 1 (FOXD2-AS1), a long noncoding RNA (lncRNA), exhibits specifically elevated in numerous cancerous cells. Numerous studies have shown that FOXD2-AS1 encourages cellular proliferation, migration and invasion. Nevertheless, the exact mechanism through which FOXD2-AS1 contributes to prostate cancer (PCa) remains unclear. Consequently, we aimed to explore the implications of FOXD2-AS1 on the growth of PCa. Initially, an elevation of FOXD2-AS1 observed in PCa cells (PC-3, DU145 and Lncap) than the prostate normal cell line RWPE2. Then, PC-3 cells were tranafected with shFOXD2-AS1, sh-Numerical Control (shNC) or FOXD2-AS1 to assess the implications of FOXD2-AS. Cell growth was measured with cell counting kit-8 (CCK8) and 5-ethynyl-2′-deoxyuridine (EDU) assays, and cell invasion and migration were assessed by Transwell assays, which demonstrated that FOXD2-AS1 silence impeded proliferation, migration and invasion of PC-3 cells. Additionally, we discovered that FOXD2-AS1 bonded with miR-206/programmed cell death protein 10 (PDCD10) trough analyzing the interaction sites of lncRNA, miRNA and protein. Then, these interaction abilities were confirmed by dual-luciferase reporter assays and RT-qPCR, suggesting FOXD2-AS1 could upregulate the amount of PDCD10 through suppressing miR-206. Furthermore, the role of FOXD2-AS1 silencing on PCa carcinogenesis were assessed. In vivo experiment, shFOXD2-AS1 led to a notable reduction in both the size and weight of PCa. These findings indicated that FOXD2-AS1 silencing effectively hindered the progression of prostate cancer. In conclusion, the upregulation of FOXD2-AS1 was observed in PCa, and the knockdown of FOXD2-AS1 could alleviated tumor development by targeting miR-206 to upregulate PDCD10 expression.https://oss.jomh.org/files/article/20230928-106/pdf/JOMH2023070601.pdffoxd2-as1prostate cancermir-206pdcd10 |
| spellingShingle | Xiong Mei Yongli Nie Jun Chen Wei Wang FOXD2-AS1 inhibits the proliferation and migration in prostate cancer: an in vitro and in vivo study Journal of Men's Health foxd2-as1 prostate cancer mir-206 pdcd10 |
| title | FOXD2-AS1 inhibits the proliferation and migration in prostate cancer:
an in vitro and in vivo study |
| title_full | FOXD2-AS1 inhibits the proliferation and migration in prostate cancer:
an in vitro and in vivo study |
| title_fullStr | FOXD2-AS1 inhibits the proliferation and migration in prostate cancer:
an in vitro and in vivo study |
| title_full_unstemmed | FOXD2-AS1 inhibits the proliferation and migration in prostate cancer:
an in vitro and in vivo study |
| title_short | FOXD2-AS1 inhibits the proliferation and migration in prostate cancer:
an in vitro and in vivo study |
| title_sort | foxd2 as1 inhibits the proliferation and migration in prostate cancer an in vitro and in vivo study |
| topic | foxd2-as1 prostate cancer mir-206 pdcd10 |
| url | https://oss.jomh.org/files/article/20230928-106/pdf/JOMH2023070601.pdf |
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