The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice
Background: GABAergic drugs can modulate the rewarding properties of morphine. The objective of this study was to evaluate the effects of isoniazid, as a GABAergic agent, on the rewarding effects of morphine. Methods: Eighteen groups of female mice (eight per group) were used in a conditioned place...
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| Language: | English |
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Kerman University of Medical Sciences
2020-01-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_89597_bff4c2b6846979bc2e387a9a4bcc8ed9.pdf |
| _version_ | 1797790733581680640 |
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| author | Amir Barzegari Kamran Shahabi |
| author_facet | Amir Barzegari Kamran Shahabi |
| author_sort | Amir Barzegari |
| collection | DOAJ |
| description | Background: GABAergic drugs can modulate the rewarding properties of morphine. The objective of this study was to evaluate the effects of isoniazid, as a GABAergic agent, on the rewarding effects of morphine. Methods: Eighteen groups of female mice (eight per group) were used in a conditioned place preference (CPP) study. On the conditioning phase of the CPP procedure, ten groups of the animals received morphine (0, 0.75, 1.5, 3, 5, and 10 mg/kg, s.c.) or isoniazid (0, 25, 50, and 75 mg/kg, i.p.) to induce CPP. Then, the effects of isoniazid on the acquisition and expression of morphine-induced CPP were evaluated. In the expression experiment, four groups of mice were conditioned with an effective dose of morphine (5mg/kg, s.c.). Then, the animals received saline or isoniazid (25, 50, and 75 mg/kg) one hour before the test, intraperitoneally. In the acquisition experiment, the other four groups received intraperitoneal saline or isoniazid (25, 50, and 75 mg/kg, i.p.) one hour before receiving the effective dose of morphine (5mg/kg, s.c.) on conditioning phase. On the test day, these animals received no treatment. Results: Morphine but not isoniazid induced a significant CPP in mice. Morphine or isoniazid alone did not change the locomotor activity of the animals on the test day. Isoniazid pretreatment could significantly inhibit both the acquisition and expression of the morphine-induced CPP. Isoniazid also did not influence the locomotor activity of the animals in the expression and acquisition experiments. Conclusion: Isoniazid may have a therapeutic application in morphine addiction. |
| first_indexed | 2024-03-13T02:08:45Z |
| format | Article |
| id | doaj.art-0abf3f0826de46d48890825244a38065 |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:08:45Z |
| publishDate | 2020-01-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-0abf3f0826de46d48890825244a380652023-07-01T05:14:57ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432020-01-01271698110.22062/jkmu.2020.8959789597The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in MiceAmir Barzegari0Kamran Shahabi1Assistant Professor, Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, East Azerbaijan Province, IR IranM.Sc. Student, Department of Biology, Faculty of Basic Science, University of Maragheh, Maragheh, East Azerbaijan Province, IR IranBackground: GABAergic drugs can modulate the rewarding properties of morphine. The objective of this study was to evaluate the effects of isoniazid, as a GABAergic agent, on the rewarding effects of morphine. Methods: Eighteen groups of female mice (eight per group) were used in a conditioned place preference (CPP) study. On the conditioning phase of the CPP procedure, ten groups of the animals received morphine (0, 0.75, 1.5, 3, 5, and 10 mg/kg, s.c.) or isoniazid (0, 25, 50, and 75 mg/kg, i.p.) to induce CPP. Then, the effects of isoniazid on the acquisition and expression of morphine-induced CPP were evaluated. In the expression experiment, four groups of mice were conditioned with an effective dose of morphine (5mg/kg, s.c.). Then, the animals received saline or isoniazid (25, 50, and 75 mg/kg) one hour before the test, intraperitoneally. In the acquisition experiment, the other four groups received intraperitoneal saline or isoniazid (25, 50, and 75 mg/kg, i.p.) one hour before receiving the effective dose of morphine (5mg/kg, s.c.) on conditioning phase. On the test day, these animals received no treatment. Results: Morphine but not isoniazid induced a significant CPP in mice. Morphine or isoniazid alone did not change the locomotor activity of the animals on the test day. Isoniazid pretreatment could significantly inhibit both the acquisition and expression of the morphine-induced CPP. Isoniazid also did not influence the locomotor activity of the animals in the expression and acquisition experiments. Conclusion: Isoniazid may have a therapeutic application in morphine addiction.https://jkmu.kmu.ac.ir/article_89597_bff4c2b6846979bc2e387a9a4bcc8ed9.pdfisoniazidmorphinerewardconditioning |
| spellingShingle | Amir Barzegari Kamran Shahabi The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice Journal of Kerman University of Medical Sciences isoniazid morphine reward conditioning |
| title | The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice |
| title_full | The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice |
| title_fullStr | The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice |
| title_full_unstemmed | The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice |
| title_short | The Effects of Isoniazid on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice |
| title_sort | effects of isoniazid on the acquisition and expression of morphine induced conditioned place preference in mice |
| topic | isoniazid morphine reward conditioning |
| url | https://jkmu.kmu.ac.ir/article_89597_bff4c2b6846979bc2e387a9a4bcc8ed9.pdf |
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