Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies
IntroductionThe tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1268376/full |
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| author | Robin D. Wright Robin D. Wright Thais F. Bartelli Seyda Baydogan James Robert White Michael P. Kim Michael P. Kim Manoop S. Bhutani Florencia McAllister Florencia McAllister Florencia McAllister Florencia McAllister |
| author_facet | Robin D. Wright Robin D. Wright Thais F. Bartelli Seyda Baydogan James Robert White Michael P. Kim Michael P. Kim Manoop S. Bhutani Florencia McAllister Florencia McAllister Florencia McAllister Florencia McAllister |
| author_sort | Robin D. Wright |
| collection | DOAJ |
| description | IntroductionThe tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult to define the TM prior to surgical resection.MethodsIn this pilot feasibility study, patients underwent Endoscopic Ultrasound-Fine Needle Aspiration (EUS-FNA) biopsy of pancreatic adenocarcinoma. These samples were analyzed using 16S rRNA and internal transcribed spacer (ITS) sequencing for characterization of the tumor bacteria and fungi.ResultAfter in silico decontamination and comparison to non-matched tumor, we were able to characterize the TM in biopsies, which was comparable to the TM from surgical specimens.DiscussionEUS-FNA biopsy may represent a feasible modality to characterize the pancreatic TM prior to surgical resection with proper decontamination strategies and improvements in matched controls. |
| first_indexed | 2024-03-11T18:33:30Z |
| format | Article |
| id | doaj.art-0ac02d25bf604d36901b4bfabb381546 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T18:33:30Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0ac02d25bf604d36901b4bfabb3815462023-10-13T07:38:34ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-10-011410.3389/fimmu.2023.12683761268376Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsiesRobin D. Wright0Robin D. Wright1Thais F. Bartelli2Seyda Baydogan3James Robert White4Michael P. Kim5Michael P. Kim6Manoop S. Bhutani7Florencia McAllister8Florencia McAllister9Florencia McAllister10Florencia McAllister11Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Clinical Cancer Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Gastrointestinal Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesDepartment of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX, United StatesIntroductionThe tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult to define the TM prior to surgical resection.MethodsIn this pilot feasibility study, patients underwent Endoscopic Ultrasound-Fine Needle Aspiration (EUS-FNA) biopsy of pancreatic adenocarcinoma. These samples were analyzed using 16S rRNA and internal transcribed spacer (ITS) sequencing for characterization of the tumor bacteria and fungi.ResultAfter in silico decontamination and comparison to non-matched tumor, we were able to characterize the TM in biopsies, which was comparable to the TM from surgical specimens.DiscussionEUS-FNA biopsy may represent a feasible modality to characterize the pancreatic TM prior to surgical resection with proper decontamination strategies and improvements in matched controls.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1268376/fulltumor microbespancreatic cancerendoscopic ultrasoundfine needle aspirationmicrobiomepancreatic adenocarcinoma |
| spellingShingle | Robin D. Wright Robin D. Wright Thais F. Bartelli Seyda Baydogan James Robert White Michael P. Kim Michael P. Kim Manoop S. Bhutani Florencia McAllister Florencia McAllister Florencia McAllister Florencia McAllister Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies Frontiers in Immunology tumor microbes pancreatic cancer endoscopic ultrasound fine needle aspiration microbiome pancreatic adenocarcinoma |
| title | Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies |
| title_full | Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies |
| title_fullStr | Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies |
| title_full_unstemmed | Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies |
| title_short | Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies |
| title_sort | bacterial and fungal characterization of pancreatic adenocarcinoma from endoscopic ultrasound guided biopsies |
| topic | tumor microbes pancreatic cancer endoscopic ultrasound fine needle aspiration microbiome pancreatic adenocarcinoma |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1268376/full |
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