The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators
Enterotoxigenic Escherichia coli produces a myriad of adhesive structures collectively named colonization factors (CFs). CS3 is a CF, which is assembled into fine wiry fibrillae encoded by the cstA-H gene cluster. In this work we evaluated the influence of environmental cues such as temperature, osm...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01685/full |
| _version_ | 1818996572219768832 |
|---|---|
| author | Miguel A. Ares Judith Abundes-Gallegos Diana Rodríguez-Valverde Leonardo G. Panunzi César Jiménez-Galicia Ma. Dolores Jarillo-Quijada María Lilia Cedillo Marìa D. Alcántar-Curiel Javier Torres Jorge A. Girón Miguel A. De la Cruz |
| author_facet | Miguel A. Ares Judith Abundes-Gallegos Diana Rodríguez-Valverde Leonardo G. Panunzi César Jiménez-Galicia Ma. Dolores Jarillo-Quijada María Lilia Cedillo Marìa D. Alcántar-Curiel Javier Torres Jorge A. Girón Miguel A. De la Cruz |
| author_sort | Miguel A. Ares |
| collection | DOAJ |
| description | Enterotoxigenic Escherichia coli produces a myriad of adhesive structures collectively named colonization factors (CFs). CS3 is a CF, which is assembled into fine wiry fibrillae encoded by the cstA-H gene cluster. In this work we evaluated the influence of environmental cues such as temperature, osmolarity, pH, and carbon source on the expression of CS3 genes. The transcription of cstH major pilin gene was stimulated by growth of the bacteria in colonization factor broth at 37°C; the presence of glycerol enhanced cstH transcription, while glucose at high concentration, high osmolarity, and the depletion of divalent cations such as calcium and magnesium repressed cstH expression. In addition, we studied the role of H-NS, CpxRA, and CRP global regulators in CS3 gene expression. H-NS and CpxRA acted as repressors and CRP as an activator of cstH expression. Under high osmolarity, H-NS, and CpxRA were required for cstH repression. CS3 was required for both, bacterial adherence to epithelial cells and biofilm formation. Our data strengthens the existence of a multi-factorial regulatory network that controls transcription of CS3 genes in which global regulators, under the influence of environmental signals, control the production of this important intestinal colonization factor. |
| first_indexed | 2024-12-20T21:31:52Z |
| format | Article |
| id | doaj.art-0ac23355b5914ed3b53f620d1dfe311b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T21:31:52Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0ac23355b5914ed3b53f620d1dfe311b2022-12-21T19:26:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-07-011010.3389/fmicb.2019.01685461596The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global RegulatorsMiguel A. Ares0Judith Abundes-Gallegos1Diana Rodríguez-Valverde2Leonardo G. Panunzi3César Jiménez-Galicia4Ma. Dolores Jarillo-Quijada5María Lilia Cedillo6Marìa D. Alcántar-Curiel7Javier Torres8Jorge A. Girón9Miguel A. De la Cruz10Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoInstitut Pasteur, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, FranceUnidad Médica de Alta Especialidad, Laboratorio Clínico, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoUnidad de Investigacioìn en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoCentro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoUnidad de Investigacioìn en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoCentro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, MexicoEnterotoxigenic Escherichia coli produces a myriad of adhesive structures collectively named colonization factors (CFs). CS3 is a CF, which is assembled into fine wiry fibrillae encoded by the cstA-H gene cluster. In this work we evaluated the influence of environmental cues such as temperature, osmolarity, pH, and carbon source on the expression of CS3 genes. The transcription of cstH major pilin gene was stimulated by growth of the bacteria in colonization factor broth at 37°C; the presence of glycerol enhanced cstH transcription, while glucose at high concentration, high osmolarity, and the depletion of divalent cations such as calcium and magnesium repressed cstH expression. In addition, we studied the role of H-NS, CpxRA, and CRP global regulators in CS3 gene expression. H-NS and CpxRA acted as repressors and CRP as an activator of cstH expression. Under high osmolarity, H-NS, and CpxRA were required for cstH repression. CS3 was required for both, bacterial adherence to epithelial cells and biofilm formation. Our data strengthens the existence of a multi-factorial regulatory network that controls transcription of CS3 genes in which global regulators, under the influence of environmental signals, control the production of this important intestinal colonization factor.https://www.frontiersin.org/article/10.3389/fmicb.2019.01685/fullCS3H-NSCRPCpxRAenterotoxigenic E. coli |
| spellingShingle | Miguel A. Ares Judith Abundes-Gallegos Diana Rodríguez-Valverde Leonardo G. Panunzi César Jiménez-Galicia Ma. Dolores Jarillo-Quijada María Lilia Cedillo Marìa D. Alcántar-Curiel Javier Torres Jorge A. Girón Miguel A. De la Cruz The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators Frontiers in Microbiology CS3 H-NS CRP CpxRA enterotoxigenic E. coli |
| title | The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators |
| title_full | The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators |
| title_fullStr | The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators |
| title_full_unstemmed | The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators |
| title_short | The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators |
| title_sort | coli surface antigen cs3 of enterotoxigenic escherichia coli is differentially regulated by h ns crp and cpxra global regulators |
| topic | CS3 H-NS CRP CpxRA enterotoxigenic E. coli |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01685/full |
| work_keys_str_mv | AT miguelaares thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT judithabundesgallegos thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT dianarodriguezvalverde thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT leonardogpanunzi thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT cesarjimenezgalicia thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT madoloresjarilloquijada thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT marialiliacedillo thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT mariadalcantarcuriel thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT javiertorres thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT jorgeagiron thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT migueladelacruz thecolisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT miguelaares colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT judithabundesgallegos colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT dianarodriguezvalverde colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT leonardogpanunzi colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT cesarjimenezgalicia colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT madoloresjarilloquijada colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT marialiliacedillo colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT mariadalcantarcuriel colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT javiertorres colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT jorgeagiron colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators AT migueladelacruz colisurfaceantigencs3ofenterotoxigenicescherichiacoliisdifferentiallyregulatedbyhnscrpandcpxraglobalregulators |