Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China
Duck farms are one of the important reservoirs of antimicrobial resistance genes (ARGs) that spread to humans and the environment. However, few studies have focused on the characteristics of antimicrobial profiles in duck farms. Here we explored the distribution characteristics and potential transmi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323006954 |
| _version_ | 1797744107526815744 |
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| author | Wenguang Xiong Jintao Yang Jiaxiong Zeng Danyu Xiao Cuihong Tong Zhenling Zeng |
| author_facet | Wenguang Xiong Jintao Yang Jiaxiong Zeng Danyu Xiao Cuihong Tong Zhenling Zeng |
| author_sort | Wenguang Xiong |
| collection | DOAJ |
| description | Duck farms are one of the important reservoirs of antimicrobial resistance genes (ARGs) that spread to humans and the environment. However, few studies have focused on the characteristics of antimicrobial profiles in duck farms. Here we explored the distribution characteristics and potential transmission mechanisms of ARGs in ducks, farm workers, and the environment in duck farms by a metagenomic approach. The results showed that the highest abundance and diversity of ARGs were found in duck manure. The abundance and diversity of ARGs in workers and environmental samples were higher than those in the control group. tet(X) and its variants were prevalent in duck farms, with tet(X10) being the most abundant. The genetic structure ''tet(X)-like + α/β hydrolase'' was found in ducks, workers, and the environment, implying that tet(X) and its variants have been widely spread in duck farms. Network analysis indicated that ISVsa3 and IS5075 might play an important role in the coexistence of ARGs and metal resistance genes (MRGs). The Mantel tests showed that mobile genetic elements (MGEs) were significantly correlated with ARG profiles. The results suggest that duck manure may be a potential hotspot source of ARGs, including tet(X) variants that spread to the surrounding environment and workers via MGEs. These results help us optimize the antimicrobials strategy and deepen our understanding of ARG spread in duck farms. |
| first_indexed | 2024-03-12T15:04:59Z |
| format | Article |
| id | doaj.art-0ac2511d97494c1d8244c11832b6bbd7 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T15:04:59Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-0ac2511d97494c1d8244c11832b6bbd72023-08-13T04:51:56ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01262115191Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern ChinaWenguang Xiong0Jintao Yang1Jiaxiong Zeng2Danyu Xiao3Cuihong Tong4Zhenling Zeng5Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, ChinaEnvironmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory, (Zhuhai), Sun Yat-sen University, Guangzhou 510006, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China; Corresponding author at: Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.Duck farms are one of the important reservoirs of antimicrobial resistance genes (ARGs) that spread to humans and the environment. However, few studies have focused on the characteristics of antimicrobial profiles in duck farms. Here we explored the distribution characteristics and potential transmission mechanisms of ARGs in ducks, farm workers, and the environment in duck farms by a metagenomic approach. The results showed that the highest abundance and diversity of ARGs were found in duck manure. The abundance and diversity of ARGs in workers and environmental samples were higher than those in the control group. tet(X) and its variants were prevalent in duck farms, with tet(X10) being the most abundant. The genetic structure ''tet(X)-like + α/β hydrolase'' was found in ducks, workers, and the environment, implying that tet(X) and its variants have been widely spread in duck farms. Network analysis indicated that ISVsa3 and IS5075 might play an important role in the coexistence of ARGs and metal resistance genes (MRGs). The Mantel tests showed that mobile genetic elements (MGEs) were significantly correlated with ARG profiles. The results suggest that duck manure may be a potential hotspot source of ARGs, including tet(X) variants that spread to the surrounding environment and workers via MGEs. These results help us optimize the antimicrobials strategy and deepen our understanding of ARG spread in duck farms.http://www.sciencedirect.com/science/article/pii/S0147651323006954Antimicrobial resistance genesDuck farmtet(X)Metagenomic |
| spellingShingle | Wenguang Xiong Jintao Yang Jiaxiong Zeng Danyu Xiao Cuihong Tong Zhenling Zeng Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China Ecotoxicology and Environmental Safety Antimicrobial resistance genes Duck farm tet(X) Metagenomic |
| title | Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China |
| title_full | Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China |
| title_fullStr | Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China |
| title_full_unstemmed | Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China |
| title_short | Metagenomic analysis of antimicrobial resistance in ducks, workers, and the environment in duck farms, southern China |
| title_sort | metagenomic analysis of antimicrobial resistance in ducks workers and the environment in duck farms southern china |
| topic | Antimicrobial resistance genes Duck farm tet(X) Metagenomic |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323006954 |
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