Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep
The present study aimed to investigate whether the presence of Tau protected Hu sheep sperm from ROS stress during storage at room temperature. The semen was diluted with extender (Tris-based) at room temperature, supplemented with different concentrations of Tau (0, 10, 20, 40, 80, or 100 mM), and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/9/2725 |
| _version_ | 1797520436036108288 |
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| author | Liuming Zhang Yanhu Wang Tariq Sohail Yan Kang Haoyuan Niu Xiaomei Sun Dejun Ji Yongjun Li |
| author_facet | Liuming Zhang Yanhu Wang Tariq Sohail Yan Kang Haoyuan Niu Xiaomei Sun Dejun Ji Yongjun Li |
| author_sort | Liuming Zhang |
| collection | DOAJ |
| description | The present study aimed to investigate whether the presence of Tau protected Hu sheep sperm from ROS stress during storage at room temperature. The semen was diluted with extender (Tris-based) at room temperature, supplemented with different concentrations of Tau (0, 10, 20, 40, 80, or 100 mM), and stored at 15 °C. Sperm quality parameters (sperm progressive motility, kinetic parameters, plasma membrane integrity rate, acrosome integrity rate, and MMP) and antioxidant parameters (ROS, MDA, SOD, CAT, and T-AOC) were evaluated during the preservation of semen. The addition of Tau, especially at a concentration of 20 mM, exerted positive effects on sperm quality parameters and antioxidant parameters compared to the sperm without Tau treatment (control group). The addition of Tau, especially at a concentration of 100 mM, exerted negative effects on sperm quality parameters and antioxidant parameters compared to the control group. Interestingly, the results indicated that the sperm acrosome integrity rate did not change during storage time. In conclusion, the addition of Tau to sperm preserved at room temperature can enhance the antioxidant ability of sperm, reduce the LPO on the 5th day, and improve the quality of semen preserved at room temperature. These results implied that Tau had potential to enhance Hu sheep sperm reproductive performance. |
| first_indexed | 2024-03-10T07:56:44Z |
| format | Article |
| id | doaj.art-0ac6bcb752ae4668be77c61c38770859 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T07:56:44Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0ac6bcb752ae4668be77c61c387708592023-11-22T11:44:37ZengMDPI AGAnimals2076-26152021-09-01119272510.3390/ani11092725Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu SheepLiuming Zhang0Yanhu Wang1Tariq Sohail2Yan Kang3Haoyuan Niu4Xiaomei Sun5Dejun Ji6Yongjun Li7Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe present study aimed to investigate whether the presence of Tau protected Hu sheep sperm from ROS stress during storage at room temperature. The semen was diluted with extender (Tris-based) at room temperature, supplemented with different concentrations of Tau (0, 10, 20, 40, 80, or 100 mM), and stored at 15 °C. Sperm quality parameters (sperm progressive motility, kinetic parameters, plasma membrane integrity rate, acrosome integrity rate, and MMP) and antioxidant parameters (ROS, MDA, SOD, CAT, and T-AOC) were evaluated during the preservation of semen. The addition of Tau, especially at a concentration of 20 mM, exerted positive effects on sperm quality parameters and antioxidant parameters compared to the sperm without Tau treatment (control group). The addition of Tau, especially at a concentration of 100 mM, exerted negative effects on sperm quality parameters and antioxidant parameters compared to the control group. Interestingly, the results indicated that the sperm acrosome integrity rate did not change during storage time. In conclusion, the addition of Tau to sperm preserved at room temperature can enhance the antioxidant ability of sperm, reduce the LPO on the 5th day, and improve the quality of semen preserved at room temperature. These results implied that Tau had potential to enhance Hu sheep sperm reproductive performance.https://www.mdpi.com/2076-2615/11/9/2725taurineHu ram semenroom temperaturesperm qualityoxidative stress |
| spellingShingle | Liuming Zhang Yanhu Wang Tariq Sohail Yan Kang Haoyuan Niu Xiaomei Sun Dejun Ji Yongjun Li Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep Animals taurine Hu ram semen room temperature sperm quality oxidative stress |
| title | Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep |
| title_full | Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep |
| title_fullStr | Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep |
| title_full_unstemmed | Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep |
| title_short | Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep |
| title_sort | effects of taurine on sperm quality during room temperature storage in hu sheep |
| topic | taurine Hu ram semen room temperature sperm quality oxidative stress |
| url | https://www.mdpi.com/2076-2615/11/9/2725 |
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