Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt
Abstract In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H2O2-induced cell death in PC12 neuronal cells and the ability to chelate bio-metals (Zn2+, Fe2+, and Cu2+). The fraction...
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| Format: | Article |
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BMC
2022-11-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-022-00897-9 |
| _version_ | 1811190445409042432 |
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| author | Arezoo Rastegari Azadeh Manayi Mahdi Rezakazemi Mahdieh Eftekhari Mahnaz Khanavi Tahmineh Akbarzadeh Mina Saeedi |
| author_facet | Arezoo Rastegari Azadeh Manayi Mahdi Rezakazemi Mahdieh Eftekhari Mahnaz Khanavi Tahmineh Akbarzadeh Mina Saeedi |
| author_sort | Arezoo Rastegari |
| collection | DOAJ |
| description | Abstract In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H2O2-induced cell death in PC12 neuronal cells and the ability to chelate bio-metals (Zn2+, Fe2+, and Cu2+). The fraction was inactive toward acetylcholinesterase (AChE); however, it inhibited the butyrylcholinesterase (BChE) with IC50 value of 68.16 µg/mL, compared with donepezil as the reference drug (IC50 = 1.97 µg/mL) via Ellman’s method. It also showed good percentage of neuroprotection (86.28% at 100 µg/mL) against H2O2-induced neurotoxicity and moderate metal chelating ability toward Zn2+, Fe2+, and Cu2+. The phytochemical study led to isolation and identification of malabaricone A (1), malabaricone C (2), 4-(4-(3,4-dimethoxyphenyl)-2,3-dimethylbutyl)benzene-1,2-diol (3), nectandrin B (4), macelignan (5), and 4-(4-(benzo[d][1,3]dioxol-5-yl)-1-methoxy-2,3-dimethylbutyl)-2-methoxyphenol (6) which were assayed for their cholinesterase (ChE) inhibitory activity. Compounds 1 and 3 were not previously reported for M. fragrans. Among isolated compounds, compound 2 showed the best activity toward both AChE and BChE with IC50 values of 25.02 and 22.36 μM, respectively, compared with donepezil (0.07 and 4.73 μM, respectively). |
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| issn | 2661-801X |
| language | English |
| last_indexed | 2024-04-11T14:51:20Z |
| publishDate | 2022-11-01 |
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| series | BMC Chemistry |
| spelling | doaj.art-0ac8647ad521472388c542ac23256b1b2022-12-22T04:17:25ZengBMCBMC Chemistry2661-801X2022-11-011611910.1186/s13065-022-00897-9Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans HouttArezoo Rastegari0Azadeh Manayi1Mahdi Rezakazemi2Mahdieh Eftekhari3Mahnaz Khanavi4Tahmineh Akbarzadeh5Mina Saeedi6Persian Medicine and Pharmacy Research Center, Tehran University of Medical SciencesMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmacognosy and Pharmaceutical Biotechnology, Faculty of Pharmacy, Kermanshah University of Medical SciencesDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesPersian Medicine and Pharmacy Research Center, Tehran University of Medical SciencesPersian Medicine and Pharmacy Research Center, Tehran University of Medical SciencesAbstract In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H2O2-induced cell death in PC12 neuronal cells and the ability to chelate bio-metals (Zn2+, Fe2+, and Cu2+). The fraction was inactive toward acetylcholinesterase (AChE); however, it inhibited the butyrylcholinesterase (BChE) with IC50 value of 68.16 µg/mL, compared with donepezil as the reference drug (IC50 = 1.97 µg/mL) via Ellman’s method. It also showed good percentage of neuroprotection (86.28% at 100 µg/mL) against H2O2-induced neurotoxicity and moderate metal chelating ability toward Zn2+, Fe2+, and Cu2+. The phytochemical study led to isolation and identification of malabaricone A (1), malabaricone C (2), 4-(4-(3,4-dimethoxyphenyl)-2,3-dimethylbutyl)benzene-1,2-diol (3), nectandrin B (4), macelignan (5), and 4-(4-(benzo[d][1,3]dioxol-5-yl)-1-methoxy-2,3-dimethylbutyl)-2-methoxyphenol (6) which were assayed for their cholinesterase (ChE) inhibitory activity. Compounds 1 and 3 were not previously reported for M. fragrans. Among isolated compounds, compound 2 showed the best activity toward both AChE and BChE with IC50 values of 25.02 and 22.36 μM, respectively, compared with donepezil (0.07 and 4.73 μM, respectively).https://doi.org/10.1186/s13065-022-00897-9AChEBChEMaceMetal chelatingMyristica fragrans Houtt.Neuroprotectivity |
| spellingShingle | Arezoo Rastegari Azadeh Manayi Mahdi Rezakazemi Mahdieh Eftekhari Mahnaz Khanavi Tahmineh Akbarzadeh Mina Saeedi Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt BMC Chemistry AChE BChE Mace Metal chelating Myristica fragrans Houtt. Neuroprotectivity |
| title | Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt |
| title_full | Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt |
| title_fullStr | Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt |
| title_full_unstemmed | Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt |
| title_short | Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt |
| title_sort | phytochemical analysis and anticholinesterase activity of aril of myristica fragrans houtt |
| topic | AChE BChE Mace Metal chelating Myristica fragrans Houtt. Neuroprotectivity |
| url | https://doi.org/10.1186/s13065-022-00897-9 |
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