Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways

Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicologica...

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Main Authors: Xiawei Yu, Caiyan Zhang, Keke Chen, Yuan Liu, Ying Deng, Wenbin Liu, Dingdong Zhang, Guangzhen Jiang, Xiangfei Li, Sib Sankar Giri, Se Chang Park, Cheng Chi
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Ecotoxicology and Environmental Safety
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0147651322012374
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author Xiawei Yu
Caiyan Zhang
Keke Chen
Yuan Liu
Ying Deng
Wenbin Liu
Dingdong Zhang
Guangzhen Jiang
Xiangfei Li
Sib Sankar Giri
Se Chang Park
Cheng Chi
author_facet Xiawei Yu
Caiyan Zhang
Keke Chen
Yuan Liu
Ying Deng
Wenbin Liu
Dingdong Zhang
Guangzhen Jiang
Xiangfei Li
Sib Sankar Giri
Se Chang Park
Cheng Chi
author_sort Xiawei Yu
collection DOAJ
description Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicological mechanism of T-2 toxin in E.sinensis. In this study, an RNA-Sequencing (RNA-seq) was used in this study to investigate the effects of feed supplementation with 0 mg/kg and 4 mg/kg T-2 toxin on the hepatopancreas transcriptome of E.sinensis and establish a hepatopancreas transcriptome library of T-2 toxin chronically exposed crabs after five weeks, where 14 differentially expressed genes (DEGs) were screened out across antioxidant, apoptosis, autophagy, glucolipid metabolism and protein synthesis. The actual expression of all the DEGs (Caspase, ATG4, PERK, ACSL, CAT, BIRC2, HADHA, HADHB, ACOX, PFK, eEFe1, eIF4ɑ, RPL13Ae) was also analyzed by real-time quantitative PCR (RT-qPCR). It was demonstrated that long-term intake of large amounts of T-2 toxin could impair antioxidant enzyme activity, promote apoptosis and protective autophagy, disrupt lipid metabolism and inhibit protein synthesis in the hepatopancreas of E.sinensis. In conclusion, this study explored the toxicity mechanism of T-2 toxin on the hepatopancreas of E.sinensis at the mRNA level, which lays the foundation for further investigation of the molecular toxicity mechanism of T-2 toxin in aquatic crustaceans.
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spelling doaj.art-0ad34985575d49ef948d1e1bf993055f2023-01-05T04:30:31ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114397Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathwaysXiawei Yu0Caiyan Zhang1Keke Chen2Yuan Liu3Ying Deng4Wenbin Liu5Dingdong Zhang6Guangzhen Jiang7Xiangfei Li8Sib Sankar Giri9Se Chang Park10Cheng Chi11Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South KoreaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of China; Corresponding author.Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicological mechanism of T-2 toxin in E.sinensis. In this study, an RNA-Sequencing (RNA-seq) was used in this study to investigate the effects of feed supplementation with 0 mg/kg and 4 mg/kg T-2 toxin on the hepatopancreas transcriptome of E.sinensis and establish a hepatopancreas transcriptome library of T-2 toxin chronically exposed crabs after five weeks, where 14 differentially expressed genes (DEGs) were screened out across antioxidant, apoptosis, autophagy, glucolipid metabolism and protein synthesis. The actual expression of all the DEGs (Caspase, ATG4, PERK, ACSL, CAT, BIRC2, HADHA, HADHB, ACOX, PFK, eEFe1, eIF4ɑ, RPL13Ae) was also analyzed by real-time quantitative PCR (RT-qPCR). It was demonstrated that long-term intake of large amounts of T-2 toxin could impair antioxidant enzyme activity, promote apoptosis and protective autophagy, disrupt lipid metabolism and inhibit protein synthesis in the hepatopancreas of E.sinensis. In conclusion, this study explored the toxicity mechanism of T-2 toxin on the hepatopancreas of E.sinensis at the mRNA level, which lays the foundation for further investigation of the molecular toxicity mechanism of T-2 toxin in aquatic crustaceans.http://www.sciencedirect.com/science/article/pii/S0147651322012374Chinese mitten crabT-2 toxinTranscriptomeHepatopancreasDiet
spellingShingle Xiawei Yu
Caiyan Zhang
Keke Chen
Yuan Liu
Ying Deng
Wenbin Liu
Dingdong Zhang
Guangzhen Jiang
Xiangfei Li
Sib Sankar Giri
Se Chang Park
Cheng Chi
Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
Ecotoxicology and Environmental Safety
Chinese mitten crab
T-2 toxin
Transcriptome
Hepatopancreas
Diet
title Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
title_full Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
title_fullStr Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
title_full_unstemmed Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
title_short Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
title_sort dietary t 2 toxin induces transcriptomic changes in hepatopancreas of chinese mitten crab eriocheir sinensis via nutrition metabolism and apoptosis related pathways
topic Chinese mitten crab
T-2 toxin
Transcriptome
Hepatopancreas
Diet
url http://www.sciencedirect.com/science/article/pii/S0147651322012374
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