Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways
Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicologica...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2023-01-01
|
Series: | Ecotoxicology and Environmental Safety |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322012374 |
_version_ | 1797961254009044992 |
---|---|
author | Xiawei Yu Caiyan Zhang Keke Chen Yuan Liu Ying Deng Wenbin Liu Dingdong Zhang Guangzhen Jiang Xiangfei Li Sib Sankar Giri Se Chang Park Cheng Chi |
author_facet | Xiawei Yu Caiyan Zhang Keke Chen Yuan Liu Ying Deng Wenbin Liu Dingdong Zhang Guangzhen Jiang Xiangfei Li Sib Sankar Giri Se Chang Park Cheng Chi |
author_sort | Xiawei Yu |
collection | DOAJ |
description | Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicological mechanism of T-2 toxin in E.sinensis. In this study, an RNA-Sequencing (RNA-seq) was used in this study to investigate the effects of feed supplementation with 0 mg/kg and 4 mg/kg T-2 toxin on the hepatopancreas transcriptome of E.sinensis and establish a hepatopancreas transcriptome library of T-2 toxin chronically exposed crabs after five weeks, where 14 differentially expressed genes (DEGs) were screened out across antioxidant, apoptosis, autophagy, glucolipid metabolism and protein synthesis. The actual expression of all the DEGs (Caspase, ATG4, PERK, ACSL, CAT, BIRC2, HADHA, HADHB, ACOX, PFK, eEFe1, eIF4ɑ, RPL13Ae) was also analyzed by real-time quantitative PCR (RT-qPCR). It was demonstrated that long-term intake of large amounts of T-2 toxin could impair antioxidant enzyme activity, promote apoptosis and protective autophagy, disrupt lipid metabolism and inhibit protein synthesis in the hepatopancreas of E.sinensis. In conclusion, this study explored the toxicity mechanism of T-2 toxin on the hepatopancreas of E.sinensis at the mRNA level, which lays the foundation for further investigation of the molecular toxicity mechanism of T-2 toxin in aquatic crustaceans. |
first_indexed | 2024-04-11T00:56:08Z |
format | Article |
id | doaj.art-0ad34985575d49ef948d1e1bf993055f |
institution | Directory Open Access Journal |
issn | 0147-6513 |
language | English |
last_indexed | 2024-04-11T00:56:08Z |
publishDate | 2023-01-01 |
publisher | Elsevier |
record_format | Article |
series | Ecotoxicology and Environmental Safety |
spelling | doaj.art-0ad34985575d49ef948d1e1bf993055f2023-01-05T04:30:31ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114397Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathwaysXiawei Yu0Caiyan Zhang1Keke Chen2Yuan Liu3Ying Deng4Wenbin Liu5Dingdong Zhang6Guangzhen Jiang7Xiangfei Li8Sib Sankar Giri9Se Chang Park10Cheng Chi11Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of ChinaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South KoreaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of China; Corresponding author.Long-term feed route exposure to T-2 toxin was proved to elicit growth retarding effects and induction of oxidative stress and apoptosis in Chinese mitten crab (Eriocheir sinensis). However, no study with a holistic perspective has been conducted to date to further describe the in-depth toxicological mechanism of T-2 toxin in E.sinensis. In this study, an RNA-Sequencing (RNA-seq) was used in this study to investigate the effects of feed supplementation with 0 mg/kg and 4 mg/kg T-2 toxin on the hepatopancreas transcriptome of E.sinensis and establish a hepatopancreas transcriptome library of T-2 toxin chronically exposed crabs after five weeks, where 14 differentially expressed genes (DEGs) were screened out across antioxidant, apoptosis, autophagy, glucolipid metabolism and protein synthesis. The actual expression of all the DEGs (Caspase, ATG4, PERK, ACSL, CAT, BIRC2, HADHA, HADHB, ACOX, PFK, eEFe1, eIF4ɑ, RPL13Ae) was also analyzed by real-time quantitative PCR (RT-qPCR). It was demonstrated that long-term intake of large amounts of T-2 toxin could impair antioxidant enzyme activity, promote apoptosis and protective autophagy, disrupt lipid metabolism and inhibit protein synthesis in the hepatopancreas of E.sinensis. In conclusion, this study explored the toxicity mechanism of T-2 toxin on the hepatopancreas of E.sinensis at the mRNA level, which lays the foundation for further investigation of the molecular toxicity mechanism of T-2 toxin in aquatic crustaceans.http://www.sciencedirect.com/science/article/pii/S0147651322012374Chinese mitten crabT-2 toxinTranscriptomeHepatopancreasDiet |
spellingShingle | Xiawei Yu Caiyan Zhang Keke Chen Yuan Liu Ying Deng Wenbin Liu Dingdong Zhang Guangzhen Jiang Xiangfei Li Sib Sankar Giri Se Chang Park Cheng Chi Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways Ecotoxicology and Environmental Safety Chinese mitten crab T-2 toxin Transcriptome Hepatopancreas Diet |
title | Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways |
title_full | Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways |
title_fullStr | Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways |
title_full_unstemmed | Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways |
title_short | Dietary T-2 toxin induces transcriptomic changes in hepatopancreas of Chinese mitten crab (Eriocheir sinensis) via nutrition metabolism and apoptosis-related pathways |
title_sort | dietary t 2 toxin induces transcriptomic changes in hepatopancreas of chinese mitten crab eriocheir sinensis via nutrition metabolism and apoptosis related pathways |
topic | Chinese mitten crab T-2 toxin Transcriptome Hepatopancreas Diet |
url | http://www.sciencedirect.com/science/article/pii/S0147651322012374 |
work_keys_str_mv | AT xiaweiyu dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT caiyanzhang dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT kekechen dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT yuanliu dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT yingdeng dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT wenbinliu dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT dingdongzhang dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT guangzhenjiang dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT xiangfeili dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT sibsankargiri dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT sechangpark dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways AT chengchi dietaryt2toxininducestranscriptomicchangesinhepatopancreasofchinesemittencraberiocheirsinensisvianutritionmetabolismandapoptosisrelatedpathways |